Investigation of GDF8 Gene Promoter in Iranian Sheep

محورهای موضوعی : Camel

م. کمانگر ¹ , ه.ا. روشنفکر ² , ح. گله‌داری ³ , آ.س. صدر ⁴ , م. ممویی ⁵ , م.ت. بیگی نصیری ⁶

1 - Department of Animal Science, Ramin Agricultural and Natural Resources University, Mollasani, Ahvaz, Iran
2 - Department of Animal Science, Ramin Agricultural and Natural Resources University, Mollasani, Ahvaz, Iran
3 - Department of Animal Science, Ramin Agricultural and Natural Resources University, Mollasani, Ahvaz, Iran
4 - Department of Animal Science, Ramin Agricultural and Natural Resources University, Mollasani, Ahvaz, Iran
5 - Department of Animal Science, Ramin Agricultural and Natural Resources University, Mollasani, Ahvaz, Iran
6 - Department of Animal Science, Ramin Agricultural and Natural Resources University, Mollasani, Ahvaz, Iran

تاریخ دریافت : 1391/05/08 تاریخ پذیرش : 1391/10/11 تاریخ انتشار : 1392/12/10

کلید واژه: PCR, promoter, Arabic and Kordi sheep, GDF8, myostatin gene,

چکیده مقاله :

Myostatin is a growth factor belonging to the TGFß superfamily. TGFß growth factors are active in the regulation of embryonic development and in tissue homeostasis in adults. Myostatin is a growth factor controlling proliferation of myoblasts in embryonic development. Mutations in coding sequences of the bovine myostatin (GDF8) gene lead to muscle hyperplasia suggesting its inhibitory function on myoblast proliferation. In bovines, loss of this gene activity has been associated with expression of the double-muscled phenotype observed in some European cattle breeds. Myostatin gene polymorphism has also been studied in sheep. Due to the role of the myostatin gene in muscle development, the objective of this study was to sequence the myostatin gene promoter and its probable mutations, which have the potential to alter myostatin gene expression. DNA from blood samples of fifteen Arabic and fifteen Kordi sheep were extracted and used to amplify a 1034bp fragment in the myostatin gene. Three mutations were observed in the myostatin gene promoter at positions 430, 450 and 530.

چکیده انگلیسی:

میوستاتین یک فاکتور رشد و عضوی از سوپر خانواده  TGFβمی‌باشد. فاکتورهای رشد TGFβ نقش تنظیمی در توسعه جنینی و هومئوستازی بافت‌ها در بلوغ دارند. جهش در نواحی کدکننده ژن میوستاتین در گاو منجر به هایپرپلازی (افزایش در تعداد فیبرها) ماهیچه‌ها و در نتیجه باعث جلوگیری در تکثیر میوبلاست‌ها می‌شود. در گاو کاهش فعالیت این ژن باعث ظهور فنوتیپ ماهیچه مضاعف می‌شود که در بعضی از نژادهای گاوهای اروپایی مشاهده می‌شود. وجود چند شکلی در ژن میوستاتین در گوسفند نیز بررسی شده است. ژن میوستاتین در توسعه ماهیچه‌ها مؤثر است. این تحقیق به‌ منظور توالی‌یابی ناحیه‌ی پروموتور ژن میوستاتین و تعیین جهش‌های احتمالی که باعث تغییر بیان ژن می‌شوند، انجام شد. در این تحقیق DNA از خون 15 گوسفند عربی و 15 گوسفند کردی استخراج گردید و برای تکثیر قطعه 1034 جفت بازی ژن میوستاتین مورد استفاده قرار گرفت. سه جهش در جایگاه 430، 450 و 530 مشاهده گردید.

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