Association between MTNR1A and CYP19 Genes Polymorphisms and Economic Traits in Kurdi Sheep

محورهای موضوعی : Camel

ز. داوری ورانلو ¹ (Department of Animal Science, Gorgan University of Agricultural Science and Natural Resources, Golestan, Iran)
س. حسنی ² (Department of Animal Science, Gorgan University of Agricultural Science and Natural Resources, Golestan, Iran)
م. آهنی آذری ³ (Department of Animal Science, Gorgan University of Agricultural Science and Natural Resources, Golestan, Iran)
ف. صمدی ⁴ (Department of Animal Science, Gorgan University of Agricultural Science and Natural Resources, Golestan, Iran)
س. زکی‌زاده ⁵ (Department of Animal Science and Veterinary, Khorasan Razavi Agricultural and Natural Resources Research and Education Center, AREEO, Mashhad, Iran)
ع.ر. خان احمدی ⁶ (Department of Animal Science, Faculty of Agricultural and Natural Resources, Gonbad University, Gonbad, Iran)

تاریخ دریافت : 1395/05/15 تاریخ پذیرش : 1395/08/25 تاریخ انتشار : 1395/12/11

کلید واژه: polymorphism, reproductive traits, growth traits, &lt, i&gt, CYP19&lt, /i&gt, gene, &lt, i&gt, MTNR1A&lt, /i&gt, gene,

چکیده مقاله :

The ovine melatonin receptor 1A (MTNR1A) and aromatase (CYP19) genes were structurally characterized and the association between their variants and reproductive and growth traits was studied in Kurdi sheep at Kurdi sheep breeding station located in Shirvan, Iran. The genomic DNA was extracted by guanidine thiocyanate-silica gel method. Polymerase chain reaction was carried out to amplify 824 bp fragment of exon 2 of MTNR1A and 140 bp fragment of the exon 3 of the ovine CYP19 genes. The PCR products were digested with restriction endonucleases RsaI for MTNR1A and BstMBI for CYP19 genes and checked by polyacrylamide gel electrophoresis for the presence of restriction sites. Two alleles were found for all the loci investigated, which were named as A and B for CYP19, and R and r for MTRN1A. Allelic frequencies for MTRN1A were 0.49 and 0.51 for R and r alleles, while in the case of CYP19 gene, frequencies were 0.475 and 0.525for A and B alleles, respectively. Association analysis did not show any significant relations between MTNR1A gene polymorphisms and litter size (LS), age at first lambing (AFL) and lambing interval (LI). Moreover, CYP19 gene polymorphism did not affect birth weight (BW), weaning weight (WW), 6, 9 and 12 months (YW) body weights, age at first lambing (AFL) and lambing interval (LI).

چکیده انگلیسی:

ژن­های گیرنده ملاتونین نوع یک (MTNRlA) و آروماتاز (CYP19) از لحاظ ساختاری مشخص گردید و ارتباط بین انواع ژنوتیپ آنها با صفات تولیدمثلی و رشد در گوسفند کردی ایستگاه پرورش و اصلاح نژاد گوسفند شیروان مورد بررسی قرار گرفت. DNA  ژنومی با استفاده از روش گوانیدین ایزوتیوسیانات-سیلیکا ژل استخراج شد. تکثیر قطعات 140 و 824 جفت بازی از اگزون 3 ژن CYP19 و نیز اگزون 2 ژن MTNRA1 توسط واکنش­های زنجیره‌ای پلیمراز انجام گرفت. هضم آنزیمی محصولات PCR با استفاده از آنزیم­های برشی BstMBI برای ژن CYP19 و RsaI برای ژن MTNRA1 صورت گرفت و جهت بررسی محصولات هضم روی ژل اکریلامید الکتروفورز شدند. دو آلل A و B برای CYP19 و r و R برای MTRN1A  در همه جایگاه­های مورد بررسی شناسایی شد. فراوانی­ آللی در ژنMTRN1A  برای آلل­هایR  و r به ترتیب 49/0 و 51/0، در حالی که در ژن CYP19، 475/0 و 525/0 به ترتیب برای آلل­های A و B بود. در بررسی ارتباط بین چند شکلی ژن MTNRA1 و صفات تعداد بره در هر زایش (LS)، سن اولین زایش (AFL) و فاصله زایش (LI)، نتایج حاکی از عدم ارتباط بین ژنوتیپ­های مشاهده شده و رکورد­های فنوتیپی بود. همچنین چند­شکلی ژن CYP19 روی صفات وزن تولد (BW)، وزن از شیر گیری (WW) وزن 6، 9 و 12 ماهگی (YW) سن اولین زایش (AFL) و فاصله زایش (LI) هیچ تأثیری نداشت.

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