Novel Single Nucleotide Polymorphisms (SNPs) in Intron 2 and Exon 3 Regions of Leptin Gene in Sumba Ongole Cattle
محورهای موضوعی : Camel
1 - Research Center for Biotechnology, Indonesian Institute of Science, Jl. Raya Bogor Km. 46, Cibinong, Bogor 16911, West Java, Indonesia
2 - Biotechnology, Indonesian Institute of Science, Jl. Raya Bogor Km. 46, Cibinong, Bogor 16911, West Java, Indonesia
کلید واژه: polymorphism, Leptin gene, Pasundan cattle, polymorphic informative content, SNPs,
چکیده مقاله :
The bovine leptin (LEP) gene was widely used as a candidate gene for molecular selection to improve productivity traits of cattle. This study was carried out to identify single nucleotide polymorphisms (SNPs) in the LEP gene of Sumba Ongole (SO, Bos indicus) cows using sequencing method. A total of 31 animals were used in this study for analyses. Research showed that total of 16 SNPs were detected in the LEP gene. Along 2025 bp of LEP gene sequence was analyzed in this study and consisted of intron 2 (1002 bp) and exon 3 (1023 bp). The polymorphic informative content (PIC) value was reached from 0.06 (low) to 0.37 (moderate). Total of 16 SNPs in LEP gene of SO cattle had moderate PIC value (0.25<PIC<0.50) and consisted of twelve SNPs in intron 2 and four SNPs in exon 3. The SNPs with moderate PIC value were detected in intron 2 (g.2325G/T; g.2423A/C; g.2448C/T; g.2456C/G; g.2466C/T; g.2778T/A; g.2857G/A) and exon 3 (g.3260T/C; g.3272T/C; g.3356C/T; g.3468G/A). The SNP of g.3468G/A as the novel SNP in LEP gene of SO cattle that not reported in other breeds of cattle. This SNP was changed the amino acid from glycine (GGG) to arginine (AGG). Two type of mutation were detected in the LEP gene of SO cattle and consisted of transversions (44%) and transitions (56%). It was concluded that the LEP gene in SO cattle was showed polymorphisme and potential for molecular selection in the breeding program through depth research.
ژن لپتین (LEP) گاوی به طور وسیعی به عنوان یک ژن کاندیدا برای انتخاب مولکولی برای بهبود صفات تولیدی گاوها استفاده شده است. این پژوهش برای تعیین چندشکلی‌های تک نوکلئوتیدی (SNPs) در ژن LEP گاوهای Sumba Ongole (SO, Bos indicus) با استفاده از روش توالی یابی انجام شد. در این پژوهش مجموعه‌ای از 31 حیوان برای تجزیه و تحلیل استفاده شدند. تحقیق نشان داد که مجموعه‌ای از 16 SNPs در ژن LEP شناسایی شدند. یک توالی 2025 جفت بازی از ژن LEP در این مطالعه بررسی شد و شامل اینترون 2 (1002 جفت باز) و اگزون 3 (1023 جفت باز) بود. مقدار محتوای اطلاعات چندشکلی (PIC) از 0.06 (پایین) تا 0.37 (متوسط) متغییر بود. مجموع 16 چندشکلی در ژن LEP گاوهای SO ارزش PIC متوسط داشتند (0.25<PIC<0.50) و شامل 12 چندشکلی در اینترون 2 و چهار چندشکلی در اگزون 3 می‌شدند. چندشکلی‌های با ارزش PIC متوسط در اینترون 2 (g.2325G/T؛ g.2423A/C؛ g.2448C/T؛ g.2456C/G؛ g.2466C/T؛ g.2778T/A؛ g.2857G/A) و اگزون 3 (g.3260T/C؛ g.3272T/C؛ g.3356C/T؛ g.3468G/A) شناسایی شدند. چندشکلی g.3468G/A به عنوان چندشکلی جدید در ژن LEP گاوهای SO معرفی شد که در دیگر نژادهای گاو گزارش نشده است. این چندشکلی باعث تغییر اسیدآمینه از گلایسین (GGG) به آرژنین (AGG) شد. دو نوع از جهش در ژن LEP گاوهای SO شناسایی شد و شامل ترانسورژن (44 درصد) و ترانزیشن (56 درصد) بود. نتیجه گرفته شد که ژن LEP در گاوهای SO چندشکلی نشان داد و دارای پتانسیل برای انتخاب مولکولی در برنامه اصلاح نژادی از طریق تحقیقات عمیق است.
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