Allele Frequency of c.486A>G Polymorphism of the AA-NAT Gene in Iranian Indigenous and Exotic Sheep Populations
محورهای موضوعی : Camel
S. Emanizadeh
1
(Department of Animal Science, Faculty of Agriculture, University of Tabriz, Tabriz, Iran)
H. Alnajm
2
(Department of Animal Production Techniques, Al-Musaib Technical College, Al-Furat Al-Awsat Technical University, Babylon, Iraq)
A. Javanmard
3
(Department of Animal Science, Faculty of Agriculture, University of Tabriz, Tabriz, Iran)
J. Shoja
4
(Department of Animal Science, Faculty of Agriculture, University of Tabriz, Tabriz, Iran)
A. Rafat
5
(Department of Animal Science, Faculty of Agriculture, University of Tabriz, Tabriz, Iran)
کلید واژه: PCR-RFLP, melatonin, <i>AA-NAT</i> gene, estrus non-breeding season,
چکیده مقاله :
Typically, lambing percentage is classified as a composite trait and is crucial to profitability in domestic sheep farming. In breeding programs, out-of-season reproduction of sheep is an important tool because seasonal reproduction limits productivity and flexibility. Understanding the complexities of genetic aspects of the none- seasonal reproduction has received significant critical attention in the literature. In this puzzle, the arylalkylamine-N-acetyltransferase (AA-NAT) is a rate-limiting enzyme of the melatonin synthesis pathway and is highlighted as a candidate gene that is responsible for melatonin synthesis and is thus directly associated with out-of-season reproduction in sheep. With this scenario research, we aimed to examine the allele frequency of the polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) polymorphism in the AA-NAT gene in 11 native and exotic sheep populations. A total of 220 blood samples were taken from 11 breeds of sheep, including the exotic breeds Romanov (ROMV) and [Awassi (AWAS), Arabi (ARAB), Naaimi (NAIM) Iraqi native sheep] and [Ghezel (GHZL), Makui (MAKU)], Kurdi (KURD), Baluchi (BALCH), Afshari (AFSR) Iranian native sheep] and two Afshari-Booroola and Romanov-Ghezel F1 cross). Here, we describe the Smal-RFLP genotypes and allele frequency patterns of the (c.486A>G) casual single nucleotide polymorphism (SNP) in the exon of AA-NAT within and between the examined sheep breeds. In addition, PCR sequencing methods were used to double-check A/G and confirmation of PCR-RFLP results. This mutation changed the Arg > Gly structure from helix to helix-effective in improving non-seasonal reproduction. Interestingly, the observed variation of G allele ranged from 0.1 to 0.43 in all study breeds. ROMV is a candidate for non-seasonal sheep breeds and its cross expresses the highest G allele frequency among other breeds studied. The frequency of the AA-NAT genotype was significantly different between breeds in this study. Therefore, the exotic 486A>G mutation created a useful mirror to identify genomics aspects of seasonal/non-seasonal reproduction in sheep.
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