Effects of Fat Supplementation and Chop Length on Milk Composition and Ruminal Fermentation of Cows Fed Diets Containing Alfalfa Silage
محورهای موضوعی : Camelم. شریفی 1 , ع. حسین خانی 2 , م. صوفی زاده 3 , ج. موسوی 4
1 - Department of Animal Science, Faculty of Agriculture, University of Tabriz, Tabriz, Iran
2 - Department of Animal Science, Faculty of Agriculture, University of Tabriz, Tabriz, Iran
3 - Department of Animal Science, Faculty of Agriculture, University of Urmia, Urmia, Iran
4 - Department of Animal Science, Faculty of Agricultural Science, University of Gilan, Rasht, Iran
کلید واژه: dairy cow, milk composition, fat supplementation, ruminal parameters,
چکیده مقاله :
The aim of this study was to evaluate the effect of fat supplementation for enhancing milk conjugated linoleic acid (CLA) in early lactation without affecting ruminal fermentation. 24 Holstein dairy cows were assigned to a completely randomized design employing a 2 × 4 factorial arrangement. Factors were particle size (15 and 25 mm) and two different sources of alfalfa supplemented with and without fat (alfalfa hay; alfalfa hay plus 3% fat; alfalfa silage and alfalfa silage plus 3% fat). The results indicated that fat supplementation had no effect on body condition score, milk yield, feeding behaviour and C18 fatty acids profile of milk. Alfalfa silage with fat increased significantly the amount of C18:1and C18:2 in rumen and milk compared to alfalfa hay with fat. Particle size had no effect on milk yield, C18 fatty acids of milk, ruminal NH3-N and butyrate in the rumen. Dry matter intake, milk fat and acetic acid declined with fat supplementation. Dietary alfalfa silage had significant effect on dry matter intake, milk fat, ruminating time and rumen fermentation. Feeding behaviour decreased with reducing particle size. Results revealed that alfalfa silage with fat increased milk yield enhanced CLA in milk improved blood and ruminal metabolite and maintained higher body weight in Holstein dairy cows.
هدف از این مطالعه بررسی اثر مکمل چربی بر افزایش اسید لینولئیک کانژوگه شیر (CLA) در اوایل شیردهی بدون تأثیر تخمیر شکمبهایی بر آن بود. 24 رأس گاو هلشتاین در یک طرح کاملاً تصادفی با فاکتوریل 2 × 4 مورد استفاده قرار گرفتند. فاکتورها شامل اندازه ذرات (15 و 25 میلیمتر) و دو منبع مختلف یونجه همراه با و بدون چربی (یونجه خشک، یونجه خشک به علاوه 3 درصد چربی، سیلوی یونجه و سیلوی یونجه به علاوه 3 درصد چربی) بود. نتایج نشان داد که مکمل چربی هیچ تأثیری در وضعیت بدنی، عملکرد شیر، رفتارهای غذایی و اسید چرب C18 شیر نداشته است. سیلوی یونجه با چربی سبب افزایش قابل توجهی در مقدار میزان C18:1 و C18:2 در شکمبه و شیر نسبت به یونجه خشک با چربی داشته است. اندازه ذرات علوفه هیچ تأثیری بر تولید شیر، اسیدهای چرب C18 شیر، ازت آمونیاکی و بوتیرات در شکمبه نداشته است. مصرف ماده خشک، چربی شیر و اسید استیک با مکمل چربی کاهش یافته است. سیلوی یونجه اثر قابل توجهی در مصرف ماده خشک، چربی شیر، زمان نشخوار و تخمیر شکمبه داشته است. رفتار تغذیه با کاهش اندازه ذرات کاهش یافته است. نتایج نشان داد که سیلوی یونجه با چربی سبب افزایش تولید شیر، توسعه CLA شیر، بهبود متابولیتهای خونی و شکمبهایی و حفظ وزن بالاتر بدن در گاوهای شیری هلشتاین می شود
AOAC. (1990). Official Methods of Analysis. Vol. I. 15th Ed. Association of Official Analytical Chemists, Arlington, VA, USA.
Beauchemin K.A., Eriksen L., Norgaard P. and M. Rode L. (2008). Short communication: salivary secretion during meals in lactating dairy cattle. J. Dairy Sci. 91, 2077-2081.
Bell J.A., Griinari J.M. and Kennelly J.J. (2006). Effect of safflower oil, flaxseed oil, monensin, and vitamin E on concentration of conjugated linoleic acid in bovine milk fat. J. Dairy Sci. 89, 733-748.
Bhandari S.K., Li S., Ominski K.H., Wittenberg K.M. and Plaizier J.C. (2008). Effects of the chop lengths of alfalfa silage and oat silage on feed intake, milk production, feeding behavior, and rumen fermentation of dairy cows. J. Dairy Sci. 91, 1942-1958.
Boerman J.P. and Lock A.L. (2014). Effect of unsaturated fatty acids and triglycerides from soybeans on milk fat synthesis and biohydrogenation intermediates in dairy cattle. J. Dairy Sci. 97, 7031-7042.
Broderick G.A. and Kang G.H. (1980). Automated simultaneous determination of ammonia and total amino acids in ruminal fluid and in vitro media. J. Dairy Sci. 63, 64-75.
Calberry J.M., Plaizier J.C., Einarson M.S. and McBride B.W. (2003). Effects of replacing chopped alfalfa hay with alfalfa silage in a total mixed ration on production and rumen conditions of lactating dairy cows. J. Dairy Sci. 86, 3611-3619.
Chouinard P.Y., Le-vesque J., Girard V. and Brisson G.J. (1997). Dietary soybeans extruded at different temperatures: milk composition and in situ fatty acid reactions. J. Dairy Sci. 80, 2913-2924.
Folch J., Lees M. and Stanley G.S.H. (1957). A simple method for the isolation and purification of lipids from animal tissues. J. Biol. Chem. 266, 497-509.
Griinari J.M. (2003). Update on theories of diet-induced milk fat depression and potential applications. Pp. 180-200 in Recent Advances in Animal Nutrition. P.C. Garnsworthy and J. Wiseman, Eds. Nottingham Univercity Press, Nottingham, UK.
Harvatine K.J. and Allen M.S. (2005). The effect of production level on feed intake, milk yield and endocrine responses to two fatty acid supplements in lactating cows. J. Dairy Sci. 88, 4018-4027.
He M., Perfield K.L., Green H.B. and Armentano L.E. (2012). Effect of dietary fat blend enriched in oleic or linoleic acid and monensin supplementation on dairy cattle performance, milk fatty acid profiles and milk fat depression. J. Dairy Sci. 95, 1447-1461.
Hollmann M. and Beede D.K. (2012). Comparison of effects of dietary coconut oil and animal fat blend on lactational performance of Holstein cows fed a high-starch diet. J. Dairy Sci. 95, 1484-1499.
Jenkins T., Wallace R., Moate P. and Mosley E. (2008). Board invited review: recent advances in biohydrogenation of unsaturated fatty acids within the rumen microbial ecosystem. J. Anim. Sci. 86, 397-412.
Jerred M.J., Carroll D.J., Combs D.K. and Grummer R.R. (1990). Effects of fat supplementation and immature alfalfa to concentrate ratio on lactation performance of dairy cattle. J. Dairy Sci. 73, 2842-2854.
Johnson M.M. and Peters J.P. (1993). An improved method to quantify nonesterified fatty acids in bovine plasma. J. Anim. Sci. 71, 753-756.
Khadem A.A., Sharifi M., Afzalzadeh A. and Rezaeian M. (2009). Effects of diets containing alfalfa hay or barley flour mixed alfalfa silage on feeding behavior, productivity, rumen fermentation and blood metabolites in lactating cows. J. Anim. Sci. 80, 403-410.
Kononoff P.J. and Heinrichs A.J. (2003). The effect of reducing alfalfa haylage particle size on cows in early lactation. J. Dairy Sci. 86, 1445-1457.
Kowsar R., Ghorbani G., Alikhani M., Khorvash M. and Nikkhah A. (2008). Corn silage partially replacing short alfalfa hay to optimize forage use in total mixed rations for lactating cows. J. Dairy Sci. 91, 4755-4764.
Larsen M., Lund P., Weisbjerg M.R. and Hvelplund T. (2009). Digestion site of starch from cereal and legumes in lactating dairy cows. Anim. Feed Sci. Technol. 153, 236-248.
Littell R.C., Henry P.R. and Ammerman C.B. (1998). Statistical analysis of repeated measures data using SAS procedures. J. Anim. Sci. 76, 1216-1231.
Mertens D.R. (1987). Predicting intake and digestibility using mathematical models of ruminal function. J. Dairy Sci. 64, 1548-1558.
NRC. (2001). Nutrient Requirements of Dairy Cattle. 7thEd.NationalAcademy Press, Washington, DC, USA.
Onetti S.G., Reynal S.M. and Grummer R.R. (2004). Effect of alfalfa forage preservation method and particle length on performance of dairy cows fed corn silage-based diets and tallow. J. Dairy Sci. 87, 652-664.
Plaizier J.C. (2004). Replacing chopped alfalfa hay with alfalfa silage in barley grain and alfalfa based total mixed rations for lactating dairy cows. J. Dairy Sci. 87, 2495-2505.
SAS Institute. (2004). SAS®/STAT Software, Release 9.1. SAS Institute, Inc., Cary, NC. USA.
Schroeder G.F., Gagliostro G.A., Becu-Villalobos D. and Lacau-MengidoI. (2002). Supplementation with partially hydrogenated oil in grazing dairy cows in early lactation. J. Dairy Sci. 85, 580-594.
Teimouri Yansari A., Valizadeh R., Naserian A. and Christensen D.A. (2004). Effects of alfalfa particle size and specific gravity on chewing activity, digestibility and performance of Holstein dairy cows. J. Dairy Sci. 87, 3912-3924.
Vagnoni D.B. and Broderick G.A. (1997). Effects of treated of energy or ruminally undegraded protein to lactating cows fed alfalfa hay or silage. J. Dairy Sci. 80, 1703-1712.
Van Soest P.J., Robertson J.B. and Lewis B.A. (1991). Methods for dietary fibre, neutral detergent fibre and non-starch polysaccharides in relation to animal nutrition. J. Dairy Sci. 74, 3583-3597.
Yang W.Z. and Beauchemin K.A. (2007). Altering physically effective fiber intake through forage proportion and particle length: digestion and milk production. J. Dairy Sci. 90, 3410-3421.