In vitro Assessment of Adsorbents to Counteract Lead Toxicity in Ruminal Fermentation
محورهای موضوعی : Camel
س. آزادبخت
1
(Department of Animal Science, College of Abouraihan, University of Tehran, Tehran, Iran)
ع.ا. خادم
2
(Department of Animal Science, College of Abouraihan, University of Tehran, Tehran, Iran)
م.ع. نوروزیان
3
(Department of Animal Science, College of Abouraihan, University of Tehran, Tehran, Iran)
کلید واژه: Sheep, rumen fermentation, Lead, Adsorbent,
چکیده مقاله :
An in vitro gas production technique was used to evaluate the effects of different adsorbents on the gas production parameters of lead-exposed diet. Ruminal fluid, obtained from fistulated sheep (3-4 years of age and 40±5 kg BW), and experimental diets samples, 200 ± 0.2 mg DM, were incubated in 100 mL glass bottles. To each bottle, one of the following treatments was applied: 1) control (no Pb and adsorbent), 2) 15 mg/kg DM Pb as Pb acetate and no adsorbent, 3) 15 mg/kg DM Pb as Pb acetate and 3% activated carbon, 4) 15 mg/kg DM Pb as Pb acetate and 3% nanoclay and 5) 15 mg/kg DM Pb as Pb acetate and 3% bentonite. Supplementation of ruminal medium with 15 ppm Pb significantly (P<0.01) decreased total and rate of gas production at all incubation times. Rate and total gas production were significantly (P<0.05) higher for nanoclay compared to control and the other adsorbents treatments. Lead inclusion decreased organic matter digestibility (OMD), net and metabolizable energy contents and short chain fatty acids production (P<0.01). Among the adsorbents, nanoclay showed to be more effective on ruminanl fermentation parameters and improved rate and total gas production.
جهت ارزیابی کارایی چند جاذب سرب، از روش آزمون گاز استفاده شد. مایع شکمبه حاصل از گوسفندان فیستوله شده به همراه 2/0 ± 200 میلی‌گرم جیره آزمایشی انکوبه شدند. تیمارهای آزمایشی شامل 1) شاهد (بدون سرب و جاذب)، 2) 15 میلی‌گرم در کیلوگرم ماده خشک استات سرب و بدون جاذب 3) 15 میلی‌گرم در کیلوگرم ماده خشک استات سرب و 3 درصد کربن فعال 4) 15 میلی‌گرم در کیلوگرم ماده خشک استات سرب و 3 درصد نانو رس و 5) 15 میلی‌گرم در کیلوگرم ماده خشک استات سرب و 3 درصد بنتونیت بودند. اضافه کردن سرب باعث کاهش میزان و نرخ تولید گاز در تمام زمان‌های انکوباسیون شد (01/0>P). میزان و نرخ تولید گاز در تیمار نانوکلی نسبت به سایر تیمارها بیشتر بود (05/0>P). افزودن سرب به محیط انکوباسیون باعث کاهش قابلیت هضم ماده آلی، مقدار انرژی قابل متابولیسم و انرژی خالص و تولید اسیدهای چرب کوتاه زنجیر شد (01/0>P). در بین جاذب‌های مختلف نانو رس تأثیر بیشتری بر فراسنجه‌های تخمیر شکمبه داشت و توانست نرخ و میزان تولید گاز را بیشتر افزایش دهد.
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