The Association of Single Nucleotide Polymorphism (SNP) g.281G > A of CAST Gene with Meat Quality of Boerka Goat

محورهای موضوعی : Camel

ا. آنتنیوس ¹ , س.پ. گینتینگ ² , س. الایزر ³ , ا. تاریگان ⁴ , س. سلهودین ⁵ , آی.گ.س. بودیساتریا ⁶ , آ.پ.ز.ن.ل. ساری ⁷ , د.ن.ه. هاریونو ⁸ , د. ماهارانی ⁹

1 - Indonesia Goat Research Center Sei Putih, Galang 20585, North Sumatera, Indonesia
2 - Indonesia Goat Research Center Sei Putih, Galang 20585, North Sumatera, Indonesia
3 - Indonesia Goat Research Center Sei Putih, Galang 20585, North Sumatera, Indonesia
4 - Indonesia Goat Research Center Sei Putih, Galang 20585, North Sumatera, Indonesia
5 - Indonesia Goat Research Center Sei Putih, Galang 20585, North Sumatera, Indonesia
6 - Department of Animal Breeding and Reproduction, Faculty of Animal Science, Universitas Gadjah Mada, Yogyakarta, Indonesia
7 - Department of Animal Breeding and Reproduction, Faculty of Animal Science, Universitas Gadjah Mada, Yogyakarta, Indonesia
8 - Department of Animal Breeding and Reproduction, Faculty of Animal Science, Universitas Gadjah Mada, Yogyakarta, Indonesia
9 - Department of Animal Breeding and Reproduction, Faculty of Animal Science, Universitas Gadjah Mada, Yogyakarta, Indonesia

تاریخ دریافت : 1398/03/28 تاریخ پذیرش : 1398/05/24 تاریخ انتشار : 1399/03/12

کلید واژه: polymorphism, Calpastatin, Boerka goat,

چکیده مقاله :

Calpastatin gene has been known as a candidate gene for meat quality in cattle, sheep, and chicken. The purpose of this study was to identify CAST gene polymorphisms and its association with meat traits in Boerka goat. The data of pH, cooking loss (CL), warner-bratzler shear force (WBSF), water holding capacity (WHC), cholesterol, water, ash, fat, and protein contains were recorded. Sequencing of 21 samples revealed five polymorphisms of CAST gene in intron 12 within Boerka goat, namely g.146C > A, g.224A > G, g.281G > A, g.737C > T, and g.431G > A. Only single nucleotide polymorphism (SNP) g.281G > A was used for genotyping. The genotype and allele frequency based on g.281G > A showed 14.29% (GG genotype) and 85.71% (GA genotype) followed with 57% G allele and 43% A allele. The chi-square test showed deviation from HWE (p <0.05) in Boerka goat. The SNP g.281G > A revealed having significantly effect to CL. The GA animals had lower CL percentage compared to the GG animals. In conclusion, the SNP selected may be used for identify meat having low CL in Boerka goat.

چکیده انگلیسی:

ژن کالپاستاتین به عنوان یک ژن کاندید برای کیفیت گوشت در گاو، گوسفند و مرغ شناخته شده است. هدف از این مطالعه شناسایی چندشکلی‌های ژن CAST و ارتباط آن با صفات گوشت در بز بوئرکا بود. داده‌های PH، از دست دادن پخت (CL)، نیروی برشی Warner-Bratzler (WBSF)، ظرفیت نگهداری آب (WHC)، کلسترول، آب، خاکستر، چربی، و محتوای پروتئینی رکورد برداری شدند. توالی‌یابی 21 نمونه، پنج چندشکلی تک‌نوکلئوتیدی در اینترون 12 ژن CAST در بز بوئرکا، به نام‌های g.146C > A، g.224A > G، g.281G > A، g.737C > T و g.431G > A نشان داد. تنها چندشکلی تک­نوکلئوتیدی (SNP) g.281G > A برای تعیین ژنوتیپ استفاده شد. فراوانی ژنوتیپی و آللی براساس g.281G > A نشان دهنده 14.29 درصد (ژنوتیپ GG) و 85.71 درصد (ژنوتیپ GA) همراه با 57 درصد آلل G و 43 درصد آلل A بود. آزمون کای-اسکوئر انحراف از تعادل هاردی-واینبرگ (HWE) (05/0P<) در بز بوئرکا را نشان داد. چندشکلی g.281G > A نشان دهنده دارا بودن اثر معنی‌دار بر CL بود. حیوانات با ژنوتیپ GA درصد CL کمتری در مقایسه با حیوانات GG داشتند. در نتیجه‌گیری، SNP انتخاب شده می‌تواند برای شناسایی گوشت دارای CL پایین در بز بوئرکا استفاده شود.

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