In vitro Effects of Exogenous Fibrolytic Enzymes on Rumen Fermentation of Wild Guinea Grass (Panicum maximum)
محورهای موضوعی : Camelاس. سوجانی 1 , آ.ن. پاتهیرانا 2 , ر.ت. سرسینهه 3 , ک.ب. داسانایاکا 4
1 - Department of Animal Science, Faculty of Agriculture, University of Ruhuna, Kamburupitiya, Sri Lanka
2 - Department of Animal Science, Faculty of Agriculture, University of Ruhuna, Kamburupitiya, Sri Lanka
3 - Department of Animal Science, Faculty of Agriculture, University of Ruhuna, Kamburupitiya, Sri Lanka
4 - School of Land and Environment, University of Melbourne, Parkville, Victoria, Australia
کلید واژه: gas production, rumen ammonia-nitrogen, rumen dry matter disappearance, rumen protozoa, volatile fatty acid,
چکیده مقاله :
This study aimed to evaluate the potential of commercial enzymes cellulase (CE), xylanase (XY) and the combination of CE + XY to improve rumen fermentation of Guinea grass ecotype ‘A’ (Panicum maximum). The experiment was carried out in a randomized complete block design. In vitro incubations were performed with four doses of individual enzymes and their combinations (1:1 from each enzyme) as 50, 100, 150 and 200 µL enzymes with 500 mg substrate. In vitro gas production (IVGP) was measured at 4 h intervals. At the end of 24 h incubation in vitro rumen dry matter disappearance (IVRDMD), ammonia nitrogen (NH3-N), protozoa population and volatile fatty acid (VFA) were estimated. Supplementation with CE, XY and CE + XY significantly enhanced IVGP (control: 38.54 mL; CET1: 50.06 mL; XYT1: 54.27 mL and CET1 + XYT1: 52.77 mL) and IVRDMD (control: 46.78%; CET1: 51.21%; XYT1: 51.53% and CE1 + XY1: 52.64%). The rumen NH3-N production was significantly increased (P<0.05) with XY and CE + XY (control: 100%; XYT1: 108.88%; and CET1 + XYT1: 111.6%). Though the total VFA did not exhibit any significant change, acetate production was significantly reduced by CE + XY while the same treatment enhanced the butyrate production. The alterations of acetate and propionate profiles led to the significantly decreased acetate: propionate with CE and CE + XY. Insignificant deduction of rumen protozoa population was observed with all enzyme treatments. In conclusion, the supplementation of exogenous fibrolytic enzyme could improve the rumen fermentation of Guinea grass ecotype ‘A’.
این مطالعه برای ارزیابی توانایی آنزیمهای تجاری سلولاز، زایلاناز و مخلوط آنها برای بهبود تخمیر شکمبهای علوفه ذرت جاروییاکوتیپ A انجام شد. آزمایش بر اساس طرح بلوکهای کامل تصادفی انجام شد. انکوباسیون داخل آزمایشگاهی با چهار دوز 50، 100، 150 و 200 میکرولیتر از آنزیمهای فردی سلولاز و زایلاناز و مخلوط آنها (با نسبت 1 به 1) با 500 میلیگرم سوبسترا انجام شد. تولید گازهای شکمبهای در فواصل چهار ساعت تعیین شدند. در پایان 24 ساعت انکوباسییون، ناپدید شدن ماده خشک، ازت آمونیاکی، جمعیت پروتوزوایی و اسیدهای چرب فرار تخمین زده شدند. مکمل کردن با سلولاز، زایلاناز و مخلوطی از آنها به طور معنیداری تولید گاز و ناپدید شدن ماده خشک را افزایش داد. تولید ازت آمونیاکی به طور معنیداری با زایلاناز و مخلوط آنها افزایش یافت. اگرچه مجموع اسیدهای چرب فرار تغییر معنیداری نشان نداد، تولید استات به طور معنیداری توسط مخلوط آنزیم سلولاز و زایلاناز کاهش یافت در حالیکه همین تیمار تولید بوتیرات را افزایش داد. تیمار سلولاز و زایلاناز منجر به کاهش معنیدار در نسبت استات به پروپیونات شد. کاهش غیر معنیداری در جمعیت پروتوزوایی شکمبه در پاسخ به تیمار آنزیمی مشاهده شد. در نتیجه، مکمل آنزیمهای بیرونی تجزیهکننده الیاف در محیط آزمایشگاه توانست تخمیر شکمبهای علوفه ذرت جارویی اکوتیپA را بهبود دهد.
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