Polymorphism of Prolactin and Prolactin Promoter Genes and Its Association with Broodiness and Body Weight in Indonesian Chicken Lines
محورهای موضوعی : Camel
ا.ب.آی. پردامایان
1
(Laboratory of Genetics and Breeding, Faculty of Biology, Universitas Gadjah Mada, Jl. Teknika Selatan, Sleman 55281, Yogyakarta, Indonesia)
ب.س. داریونو
2
(Laboratory of Genetics and Breeding, Faculty of Biology, Universitas Gadjah Mada, Jl. Teknika Selatan, Sleman 55281, Yogyakarta, Indonesia)
کلید واژه: Broiler, Prolactin, pelung, RLFP, SSCP,
چکیده مقاله :
In the world of poultry farming, female chicken positioned as parent stock must exhibit excellent laying performance without broodiness and inherit body weight related traits to her offspring. Prolactin is widely accepted to have an important role in the onset and maintenance of broodiness event. The aim of this study is to identify a valuable genetic marker for studying the polymorphism of prolactin (Prl) and prolactin promoter (Prl-P) in Indonesian chicken lines using morphology and molecular data. Experimental chicken lines were produced from broiler (non-broodiness chicken line) and pelung (Indonesian native chicken; broodiness chicken line) selective breeding for five generations. The broodiness event and body weight of each generation of chicken lines were observed and compared with Brown Leghorn as a non-broodiness outgroup. The body weight of chickens were measured weekly from hatch to 49 days of age. Molecular data were archived using restriction fragment length polymorphism (RLFP) and single-strand conformation polymorphism (SSCP). After five generations of genetics selection, at 49 days, the body weight of experimental chicken lines and growth rate were decreased, while broody behavior occurrence was diminished. RFLP and SSCP analysis failed to differentiate broody to non-broody genotype and excellent to poor body weight in each generation due to the non-specific primer. Our conclusion is that broodiness is a recessive trait and can be eliminated by selective breeding.
در دنیا مرغداری، جوجه‌های ماده انتخاب شده به عنوان هسته والدین باید عملکرد تخمگذاری عالی بدون broodiness نشان دهند خصوصیات مربوط به وزن بده را به فرزندان خود به ارث برسانند. پرولاکتین به طور گسترده به داشتن یک نقش مهم در شروع و نگهداری وقوع broodiness پذیرفته شده است. هدف از این مطالعه شناسایی یک نشانگر ژنتیکی ارزشمند برای مطالعه چندشکلی پرولاکتین (Prl) و پروموتر پرولاکتین (Prl-P) در لاین‌های مرغ اندونزیایی با استفاده از داده‌های ریخت‌شناسی و مولکولی است. لاین‌های مرغ آزمایشگاهی از سویه گوشتی (لاین مرغ بدون broodiness) و پلانگ (مرغ بومی اندونزیایی: لاین مرغ broodiness) با برنامه انتخابی برای پنج نسل تولید شدند. وقوع broodiness و وزن بدن هر نسل از لاین‌های مرغ مشاهده شده و با لگهورن قهوه‌ای به عنوان یک بدون broodiness خارج گروهی مقایسه شدند. وزن بدن مرغ‌ها هفتگی از زمان هچ تا سن 49 روزگی اندازه‌گیری شدند. داده‌های مولکولی با استفاده از چندشکلی قطعات طولی قابل هضم (RFLP) و چندشکلی آرایش فضایی تک رشته‌ای (SSCP) بایگانی شدند. بعد از پنج نسل از انتخاب ژنتیکی، در روز 49، وزن بدن لاین‌های مرغ آزمایشگاهی و نرخ رشد کاهش یافت، اگرچه وقوع رفتار broody کاهش داشت. آنالیز RFLP و SSCP نتوانست تفاوتی بین ژنوتیپ broody و غیر broody و وزن عالی تا ضعیف در هر نسل به دلیل پرایمر غیراختصاصی قائل شود. نتیجه‌گیری ما این است که broodiness یک صفت مغلوب است و می‌تواند توسط پرورش انتخابی محدود شود.
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