Statistical Models for Assessing the Influence of Hygienic Behaviour of Worker Bees on the Level of Lysozyme and Total Protein Content in Their Hаemolymph
محورهای موضوعی : Camel
س. لازاروو
1
,
پ. ولوا
2
,
آی. ژلیازکووا
3
1 - Department of Animal husbandry–Non Ruminants and other Animals, Faculty of Agriculture, Trakia University, 6000 Stara Zagora, Bulgaria
2 - Department of Informatics and Mathematics, Faculty of Economics, Trakia University, 6000 Stara Zagora, Bulgaria
3 - Department of Animal husbandry–Non Ruminants and other Animals, Faculty of Agriculture, Trakia University, 6000 Stara Zagora, Bulgaria
کلید واژه: total protein, Honeybees, hygienic behaviour, lysozyme level,
چکیده مقاله :
The hygienic behaviour of 26 bee colonies of apiaries in different regions of Bulgaria was tested. The degree of expression of hygienic behaviour was evaluated by the pin-killing method. A test field of square 5 x 5 cm was stuck onto a section of a honeycomb with a sealed worker brood. The bee colonies are divided into two groups: hygienic (cleaned over 95% of cells in the testing area on the 48th hour) and nonhygienic (cleaned less than 95% of the cells in the testing area on the 48th hour). Haemolymph has been taken from bees from each bee colony at 48th hour and lysozyme levels and total protein content has been defined. Multivariate analysis (ANOVA) was used to determine significant differences between hygienic and nonhygienic colonies. The results obtained show significant differences between both groups (p <0.05) according to percent cleaned cells after killing the brood at 3rd, 24th and 48th hours and insignificant differences between hygienic and nonhygienic colonies (p <0.05) for both parameters-lysozyme and total protein. Bivariate correlation was applied to investigate the impact of hygienic behaviour on lysozyme levels and total protein content. Low negative correlation (r=-0.33) between total protein and hygienic behaviour was established which means that an increase of the activities related to the cleansing of honeycomb cells leads to a decrease in the total protein content. Low correlation (r=0.35) between hygienic behaviour and lysozyme levels was found i.e. higher levels of lysozyme are reported with more pronounced hygiene behaviour. The calculated regression model for lysozyme (P=0.24826) is statistically insignificant but the regression model for total protein is statistically significant (P=0.003153) and can be used to predict the relation between total protein content in the haemolymph and hygienic behaviour of bee colonies.
رفتار بهداشتی 20 کلنی زنبور عسل در مناطق مختلف بلغارستان آزمون شد. درجه بیان رفتار بهداشتی توسط روش pin-killing ارزیابی شد. یک آزمون مزرعه‌ای مربع 5 × 5 سانتیمتری نصبشده روی بخشی از شان عسل با یک بچه کارگر چسبیده انجام شد. کلنی‌های زنبور به دو گروه تقسیم شدند: بهداشتی (پاک کردن بیش از 95 درصد سلولها در منطقه مورد آزمون در طی 48 ساعت) و غیربهداشتی (تمیز کردن کمتر از 95 درصد سلولها در منطقه مورد آزمون در 48 ساعت). همولنف از زنبورها از هر کلنی زنبور در 48 ساعت گرفته شد و سطوح لیزوزیم و محتوای پروتئین تام تعریف شد. آنالیز چندمتغییره (ANOVA) برای تعیین تفاوت معنی‌دار بین کلنی‌های بهداشتی و غیربهداشتی استفاده شد. نتایج بهدست آمده تفاوت‌های معنی‌داری بین دو گروه (P<0.05) براساس درصد سلول‌های تمیز شده بعد از کشتن بچه در ساعت‌های 3، 24 و 48 و تفاوت‌های معنی‌دار بین کلنی‌های بهداشتی و غیربهداشتی (P<0.05) برای هر دو پارامتر لیزوزیم و پروتئین تام نشان داد. همبستگی دو متغییره برای بررسی تأثیر رفتار بهداشتی روی سطوح لیزوزیم و محتوای پروتئین تام بکار گرفته شد. همبستگی ژنتیکی منفی (r=-0.33) بین پروتئین تام و رفتار بهداشتی بدست آمد که به معنی آن است که افزایش فعالیت‌های مربوط به پاک‌سازی سلول‌های شانه زنبوری منجر به کاهش محتوی پروتئین تام می‌شود. همبستگی پایین (r=0.35) بین رفتار بهداشتی و سطوح لیزوزیم یافت شد بدین معنی که سطوح بالاتر لیزوزیم با رفتار بهداشتی بارزتری گزارش شده است. مدل رگرسیونی محاسبه شده برای لیزوزیم (P=0.24826) به لحاظ آمتری غیرمعنی‌دار است اما مدل رگرسیونی برای پروتئین تام به طور آماری معنی‌دار است (P=0.003153) و می‌تواند برای پیش‌بینی ارتباط بین محتوی پروتئین تام در همولنف و رفتار بهداشتی کلنی‌های زنبور استفاده شود.
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