The Influence of Growth Hormone Gene Polymorphism on Growth Rate of Young Cattle

محورهای موضوعی : Camel

ت.ا. سدیخ ¹ , ای.ی. دولماتووا ² , ف.ر. والیتوو ³ , ر.س. گیزاتولین ⁴ , ل.ا. کالاشینکووا ⁵

1 - Department of Animal Breeding, Ufa Branch of the Russian Academy of Science, Bashkir State Agrarian Institute, Ufa, Russia
2 - Department of Animal Science and Breeding, Bashkir State Agrarian University, Ufa, Russia
3 - Department of Animal Science and Breeding, Bashkir State Agrarian University, Ufa, Russia
4 - Department of Animal Science and Breeding, Bashkir State Agrarian University, Ufa, Russia
5 - All-Russian Breeding Research Institute, Moscow, Russia

تاریخ دریافت : 1398/06/05 تاریخ پذیرش : 1398/07/23 تاریخ انتشار : 1399/06/11

کلید واژه: PCR-RFLP, polymorphism, growth rate, GH, Bestuzhev breeds, Black-and-White, growth hormone gene, Hereford, Limousine,

چکیده مقاله :

Beef production is an important development area in animal breeding with meat quality being determined by both paratypic and genetic factors. In this regard evaluating genetic material for the presence of desirable allele combinations of genes associated with growth and development indicators, as well as meat qualities of animals have a certain scientific and practical significance. The aim of the study was to determine the influence of growth hormone gene polymorphism on the growth rate of bull calves of different breeds kept in the Bashkortostan Republic. The method of polymerase chain reaction (PCR) followed by the analysis of restriction fragment length polymorphism (PFLP) (SNP GH-L127V) was used to genotype Hereford (115 heads), Limousine (114 heads), Black-and-White (200 heads), Bestuzhev (200 heads) bull calves being fattened. The conducted research showed that there is a similar distribution of genotypes among bull calves of meat breeds with GHLL homozygous genotype being more common (47.83% and 52.63%). Black-and-White and Bestuzhev bull calves have a higher frequency of GHLV heterozygous genotype (62.50% and 59.0%). Hereford, Limousine and black-and-white bulls have a greater frequency of GHL allele (0.69; 0.71; 0.51), Bestuzhev animals have a higher rate of GHV allele (0.62). The paper presents the influence of somatotropin hormone gene polymorphism on some meat productivity and growth rate indicators of young animals. Thus, GHLL-genotyped Hereford, Limousine, and Black-and-White bull calves had significantly higher live weight (pre-slaughter live weight) as well as absolute and average daily live weight gains at the end of rearing. According to the results GHLV-genotyped animals were in the second place and GHVV-genotyped bulls ranked the last. As a result of the one-way analysis of variance, there has been found a high proportion of the studied polymorphism factor in developing meat productivity and growth rate indicators for Limousine, Black-and-White and Hereford bull calves. Genotyping by the GH gene as an additional criterion can be used in the selection of animals to improve cattle meat quality.

چکیده انگلیسی:

تولید گوشت یک منطقه توسعه‌ای مهم در پرورش حیوانات است با کیفیت گوشت که توسط فاکتورهای پاراتیپیک و ژنتیکی تعیین می‌شود. از این‌ رو ارزیابی ماده ژنتیکی برای حضور ترکیبات آللی مطلوب ژن‌های مرتبط با رشد شاخص‌های توسعه و همچنین کیفیت گوشت حیوانات از اهمیت علمی و عملی خاصی برخوردار است. هدف از این مطالعه تعیین تأثیر چندشکلی ژن هورمون رشد روی نرخ رشد گوساله‌های نر نژادهای مختلف نگهداری شده در جمهوری باشقیرستان بود. روش واکنش زنجیره‌ای پلیمراز (PCR) به همراه آنالیز چندشکلی قطعات طولی قابل هضم (RFLP) (SNP GH-L127V) برای تعیین ژنوتیپ گوساله‌های نر پرواری هرفورد (115 رأس)، لیموزین (114 رأس)، سیاه و سفید (200 رأس) و بستوژو (200 رأس) استفاده شد. پژوهش انجام شده نشان داد که یک توزیع مشابه ژنوتیپی بین گوساله‌های نر نژادهای گوشتی وجود دارد با ژنوتیپ هموزیگوت GHLLمتداول‌تر (47.83 درصد و 52.63 درصد). گوساله‌های نر سیاه و سفید و بستوژو فراوانی بالاتر از ژنوتیپ هموزیگوت GHLVداشتند (62.50 درصد و 59.0 درصد). نرهای هرفورد، لیموزین و سیاه و سفید فراوانی بالاتری از آلل GHLداشتند (0.69، 0.71 و 0.51)، حیوانات بستوژو نرخ بالاتری از آلل GHv(0.62) داشتند. مقاله تأثیر چندشکلی ژن هورمون سوماتوتروپین روی برخی از شاخص‌های بهره‌وری گوشت و نرخ رشد حیوانات جوان ارائه می‌دهد. بنابراین، گوساله‌های نر ژنوتیپ شده GHLLهرفورد، لیموزین، و سیاه و سقید وزن زنده قابل توجه بالاتری (وزن زنده پیش از کشتار) و همچنین افزایش وزن زنده مطلق و متوسط روزانه در پایان دوره پرورش داشتند. بر اساس نتایج، حیوانات ژنوتیپ شده GHLVدر مکان دوم و نرهای ژنوتیپ شده GHVVرتبه آخر را داشتند. به عنوان نتیجه آنالیز واریانس یک سویه، نسبت بالایی از فاکتور چندشکلی مورد مطالعه در توسعه شاخص‌های بهره‌وری گوشت و نرخ رشد برای گوساله‌های نر لیموزین، سیاه و سفید و هرفورد یافت شده است. ژنوتیپ ژن GH به عنوان یک معیار فرعی می‌تواند در انتخاب حیوانات برای بهبود کیفیت گوشت گاو مورد استفاده قرار گیرد.

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