Genetic Diversity between Bali Cattle (Bos javanicus) and It’s Hybrids Using Microsatellite Markers
محورهای موضوعی : Camelج. جاکاریا 1 , ا. آلیاه 2 , ف. سپوترا 3 , م. بیهقی 4 , ر.ر. نور 5
1 - Department of Animal Production and Technology, Faculty of Animal Science, Institut Pertanian Bogor University, Bogor, Indonesia
2 - Goat Research Station, Sei Putih, North Sumatera, Indonesia
3 - Indonesian Research Institute for Animal Production, Bogor, Indonesia
4 - Department of Animal Production and Technology, Faculty of Animal Science, Institut Pertanian Bogor University, Bogor, Indonesia
5 - Department of Animal Production and Technology, Faculty of Animal Science, Institut Pertanian Bogor University, Bogor, Indonesia
کلید واژه: genetic diversity, microsatellite marker, Bali cattle,
چکیده مقاله :
The aim of this study was to evaluate the Bali cattle genetic diversity and hybrid cattle using microsatellite markers. Blood samples (n=192) were collected from various cattle breed, i.e. Bali (n=96), Madura (n=48), and Peranakan Ongole (PO) Kebumen (n=48). The microsatellite locus used for assessing the genetic variation was INRA035, ILSTS006, ETH225, and HEL9, while the genetic profile was described using GenAlEX, Cervus, MEGA6, structure and R programs. As a result, 46 alleles were found at four microsatellite loci studied. The genetic diversity of Bali cattle from Bali island (Ho=0.337) and Nusa Penida island (Ho=0.375) were recorded lower than that of the hybrids, i.e. Madura (Ho=0.747) and PO Kebumen (Ho=0.567) cattle breeds. Specifically, we found 283 bp (locus ILSTS006), 194 bp (locus ETH225), 147 bp and 151 bp (locus HEL9) demonstrating the distinctive alleles for Bali cattle. Also, our experimental data showed that microsatellite markers used allowed us to produce an obvious differentiation of the cattle cluster between Bali cattle and the hybrids, which was meaningful for future cattle breeding program.
هدف از این مطالعه ارزیابی تنوع ژنتیکی گاوهای Bali و گاوهای هیبرید با استفاده از نشانگرهای ریزماهواره بود. نمونه‌های خون (192n=) از نژادهای گاو متفاوت جمعآوری شد، برای نمونه Bali (n=96)، Madura (n=46)، وPeranakan Ongole (PO) Kebumen (n=48). جایگاه ژنی ریزماهواره استفاده شده برای دست‌یابی به تنوع ژنتیکی INRA035، ILSTS006، ETH225 و HEL9 بود، اگرچه پروفایل ژنتیکی با استفاده از برنامه‌های GenAlEX، Cervus، MEGA6، structure و R توضیح داده شدند. به عنوان یک نتیجه، 46 آلل در چهار لوکوس ریزماهواره مطالعهشده یافت شد. تنوع ژنتیکی گاوهای Bali از جزیره Bali (H0=0.337) و جزیره Nusa Penida (H0=0.375) پایین‌تر از هیبریدها رکوردبرداری شدند، برای نمونه گاوهای نژاد Madura (H0=0.474) و PO Kebumen (H0=0.567). اختصاصاً، ما یافتیم 283 جفت باز (لوکوس ILST006)، 194 جفت بازی (لوکوس ETH225)، 147 جفت بازی و 151 جفت بازی (لوکوس HEL9) نشان دهنده آلل‌های متمایز برای گاوهای Bali. همچنین، داده‌های آزمایشگاهی ما نشان داد که نشانگرهای ریزماهواره استفاده شده اجازه می‌دهد به ما برای تولید یک تفرق آشکار از خوشه گاوها بین گاوهای Bali و هیبریدها، که برای برنامه‌های اصلاح نژادی آینده گاوها معنی‌دار بود.
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