Calpastatin Gene Polymorphism in Raini and Tali Goat in the Kerman Province
محورهای موضوعی : Camel
1 - Department of Animal Science, Technical and Vocational University, Kerman, Iran
2 - Department of Animal Science, Faculty of Agriculture, University of Zabol, Zabol, Iran
کلید واژه: Tali goat, genotype, calpastatin gene, PCR RFLP, Raeini goat,
چکیده مقاله :
Restriction fragment length polymorphisms (RFLP) have been identified at the goat. The objective of the present study was to determine polymorphisms of calpastatin locus in Raini and Tali goats of the goats in Kerman Province, improving meat quality traits superior meat quality by selection. Calpastatin gene effect on quality and tenderness meat, to identify different genotype of this gene was randomly selected 150 Tali and 150 Raini goats, and blood samples were collected from total of animals using ethylenediaminetetraacetic acid (EDTA) tubes. DNA was extracted from blood according to DNA preparation kit to determine the genetic relationship among studied goat animals. Amplification was performed using polymerase chain reaction (PCR). Two alleles (A and B) and three genotypes, (AA, BB and AB) were observed. The frequencies of the observed genotypes for Tali and raini were 38.4, 9.6, 52 and 17, 71, 12 for AA, BB and AB, respectively. And allele frequencies were 0.47, 0.53 and 0.12, 0.88 for A and B, in Tali and Raini goats respectively.
در این مطالعه چند‌شکلی ژن کالپاستاتین در بز تالی و رایینی استان کرمان مورد بررسی قرار گرفت. ژن کالپاستاتین بر روی کیفیت و تردی گوشت تأثیر دارد. برای تعیین فراوانی ژنی و ژنوتیپی این ژن به طور تصادفی 150 بز رایینی و 150 بز تالی انتخاب گردید و کلیه نمونه خون‌ها درون لوله‌های حاوی EDTA قرار گرفت و از نمونه خون‌ها، به منظور بررسی چند‌شکلی ژنوتیپی در بزها با استفاده از کیت DNA استخراج شد. نمونه DNA برای انجام PCR استفاده گردید. دو الل (A, B) و سه ژنوتیپ (AA, AB, BB) در این مطالعه مشاهده شد. نتایج فراوانی ژنوتیپی برای ژن کالپاستاتین در بز تالی و راینی 52، 6/9، 4/38 و 12، 71 و 17 به ترتیب برای ژنوتیپ‌های AA, BB و BB تعیین شد. میزان فراوانی اللی A و B، 53/0، 47/0 و 88/0، 12/0 برای بز تالی و بز رایینی به ترتیب مشاهده گردید و همچنین نشان داده شد که روش RFLP یک روش مناسب برای بررسی چند‌شکلی ژن کالپاستاتین در بز می‌باشد.
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