Polymorphism of the Growth Hormone Gene and Its Effect on Production and Reproduction Traits in Goat
محورهای موضوعی : Camel
ف. غلامحسین زاده گوکی
1
(Department of Animal Science, Faculty of Agriculture, Shahid Bahonar University of Kerman, Kerman, Iran)
م.ر. محمدآبادی
2
(Department of Animal Science, Faculty of Agriculture, Shahid Bahonar University of Kerman, Kerman, Iran)
م. اسدی فوزی
3
(Department of Animal Science, Faculty of Agriculture, Shahid Bahonar University of Kerman, Kerman, Iran)
کلید واژه: PCR-RFLP, Growth hormone, birth and fleece weight, birth type, Raini Cashmere goat,
چکیده مقاله :
Growth hormone (GH) has an effect on a broad variety of physiological parameters such as lactation, reproduction, growth and metabolism. Thus, the aim of this study was to detect GH gene polymorphism and its association with breeding values of production and reproduction traits in Raini Cashmere goat. Breeding values were estimated using records on 26731 Raini Cashmere goats. To study GH gene polymorphism, 300 animals were selected based on their estimated breeding values (EBVs) for these traits. Then the animal’s genotype was determined using PCR-RFLP. The genotype frequencies for AA, AB and BB were 0.15, 0.85 and 0, respectively. The number of observed alleles, number of effective alleles, expected heterozygosity, observed heterozygosity, mean of heterozygosity, expected homozygosity, observed homozygosity, Nei’s index, Shanon’s index and Fixation index (Fis) were 2, 1.96, 0.49, 0.85, 0.49, 0.51, 0.15, 0.49, 0.69 and -0.74, respectively. Results showed that mean estimated breeding values for birth type, fleece weight and birth weight traits in different genotypes varies, of course these differences were not statistically significant (P>0.05). However, for fleece weight and birth type traits AB genotype had higher EBV. Due to the relatively high diversity of growth hormone gene in Raini Cashmere goat and its association with important economic traits, using growth hormone gene in breeding programs of this breed can lead to acceptable genetic progress and applying AA genotype for birth weight trait and AB genotype for fleece weight and birth type traits can be used as an indirect marker for selection of superior animals.
هورمون رشد بر انواع گوناگونی از پارامترهای فیزیولوژیکی از قبیل شیردهی، تولید مثل، رشد و متابولیسم اثر دارد. بنابراین، هدف این مطالعه تشخیص چندشکلی ژن هورمون رشد و همبستگی آن با ارزش های اصلاحی صفات تولیدی و تولیدمثلی بز کرکی راینی بود. ارزش های اصلاحی با استفاده از رکوردهای 26731 بز کرکی راینی برآورد شدند. برای مطالعه چندشکلی ژن هورمون رشد 300 حیوان بر اساس ارزشهای اصلاحی برآورد شده برای این صفات انتخاب شدند. سپس ژنوتیپ حیوانات با استفاده از تکنیک PCR-RFLP تعیین شد. فراوانی های ژنوتیپی برای ژنوتیپ های AA، AB و BB به ترتیب 0.15، 0.85 و 0 بود. تعداد آلل های مشاهده شده، تعداد آلل های مؤثر، هتروزیگوسیتی مورد انتظار، هتروزیگوسیتی مشاهده شده، میانگین هتروزیگوسیتی، هموزیگوسیتی مورد انتظار، هموزیگوسیتی مشاهده شده، شاخص نئی، شاخص شانون و شاخص تثبیت به ترتیب 2، 1.96، 0.49، 0.85، 0.49، 0.51، 0.15، 0.49، 0.69 و 0.74- به دست آمد. نتایج نشان داد که میانگین ارزش های اصلاحی برآورد شده برای نوع تولد، وزن کرک و وزن تولد در ژنوتیپ های مختلف متفاوت است، البته این تفاوت ها از نظر آماری معنی دار نبود (0.05<P). اما، برای صفات وزن کرک و نوع تولد ژنوتیپ AB ارزش اصلاحی برآورد شده بالاتری داشتند. به علت تنوع نسبتا بالای ژن هورمون رشد در بز کرکی راینی و همبستگی آن با صفات مهم اقتصادی، استفاده از ژن هورمون رشد در برنامه های اصلاح نژادی این نژاد می تواند منجر به پیشرفت ژنتیکی قابل قبولی شود و ژنوتیپ AA برای صفت وزن تولد و ژنوتیپ AB برای صفات وزن کرک و نوع تولد می توانند به عنوان نشانگر غیر مستقیم برای انتخاب حیوانات برتر استفاده شوند.
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