Sperm Characteristics in Cryopreserved Buffalo Bull Semen and Field Fertility
محورهای موضوعی : Camelکا.جی.ام. محمود 1 , آ.آ.ای. ال-سکاری 2 , ام.ای.آ. آبو ال-روس 3 , آ.دی. عبد الغفار 4 , ام. ناویتو 5
1 - Department of Animal Reproduction and Artificial Insemination, National Research Center, Dokki, Giza, Egypt
2 - General Organization for Veterinary Services, Dokki,Giza, Egypt
3 - Department of Theriogenology, Faculty of Veterinary Medicine, Benha University, Kaliobia, Egypt
4 - Department of Theriogenology, Faculty of Veterinary Medicine, Benha University, Kaliobia, Egypt
5 - Department of Animal Reproduction and Artificial Insemination, National Research Center, Dokki, Giza, Egypt
کلید واژه: buffalo bull, in vivo fertility, membrane integrity, semen quality,
چکیده مقاله :
Semen quality and its relationship to fertility are major concern in animal production. The aim of this study was to assess the relationship between frozen thawed sperm characteristics and fertility following in vivo fertility through Artificial Insemination(AI). Semen samples were collected from four buffalo bulls. Semen volume and sperm concentration appeared to be significantly different (P<0.05) among bulls, while sperm motility and live sperm percentage did not vary in fresh semen. Frozen thawed semen was evaluated for motility, viability, sperm abnormalities, membrane integrity and in vivo fertility. A significant variation was found in semen parameters among bulls after thawing. Highly significant (P>0.001) differences in membrane integrity between fresh vs. frozen semen samples were noticed. Pregnancy rate was significantly (P<0.05) different among bulls. Significant correlations were found between motility and sperm abnormalities (r=-0.64; P<0.05) and membrane integrity (r=0.64; P<0.05). A significant negative correlation (r=-0.73; P<0.01) has been reported between sperm abnormalities and membrane integrity. In addition, a correlation between pregnancy rate and live sperm percentages (r=0.65; P<0.05), has also been reported. In conclusion, in this study motility was correlated with sperm abnormalities and membrane integrity. Live sperm percentage was the only parameter correlated with fertility. Motility and live sperm percentage can be used as a predicative measure in semen evaluation.
کیفیت منی و ارتباط آن با باروری از اهمیت زیادی برخوردار است. هدف این مطالعه ارزیابی ارتباط بین خصوصیات اسپرم منجمد یخ‌گشایی شده و باروری از طریق تلقیح مصنوعی بود. نمونه‌های منی از چهار گاو میش تهیه شدند. حجم منی و غلظت اسپرم به طور معنی‌داری میان نرها متفاوت بود، در حالی که قابلیت تحرک‌پذیری اسپرم و درصد زنده‌مانی آن تفاوتی با اسپرم‌های تازه نداشت. اسپرم‌های منجمد یخ‌گشایی شده برای تحرک‌پذیری، زنده‌مانی، غیر طبیعی بودن، یکپارچگی غشاء و باروری روی دام مورد ارزیابی قرار گرفتند. تفاوت معنی‌داری در پارامترهای اسپرم گاومیش‌های نر بعد از یخ‌گشایی دیده شد. تفاوت‌های خیلی معنی‌داری در یکپارچگی غشاء بین اسپرم تازه در مقایسه با اسپرم منجمد تشخیص داده شد. نرخ آبستنی به طور معنی‌داری بین گاومیش‌های نر تفاوت داشت. همبستگی معنی‌داری بین تحرک‌پذیری و غیر طبیعی بودن اسپرم (64/0r=-) و یکپارچگی غشاء اسپرم (64/0r=-) یافت شد. همبستگی منفی (73/0r=-) معنی‌داری بین غیر طبیعی بودن اسپرم و یکپارچگی غشا گزارش شده است. علاوه بر این، یک همبستگی بین نرخ آبستنی و درصد اسپرم‌های زنده (65/0r=-) نیز گزارش شده است. در این مطالعه، تحرک‌پذیری اسپرم با غیر طبیعی بودن و یکپارچگی غشاء ارتباط داشت. درصد اسپرم زنده، تنها پارامتر همبسته با باروری بود. تحرک‌پذیری و درصد اسپرم زنده می تواند به عنوان یک معیار پیش‌بینی در ارزیابی اسپرم استفاده شود.
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