Responses of alfalfa influenced by magnetic field and rhizobial inoculant
محورهای موضوعی : Plant PhysiologyNeda Kazemi Khaledi 1 , Sara Saadatmand 2 , Ramazan Ali Khavari-Nejad 3 , Taher Nejadsattari 4
1 - Department of Biology, Islamic Azad University, Science and Research Branch, Tehran, Iran
2 - Department of Biology, Islamic Azad University, Science and Research Branch, Tehran, Iran
3 - Department of Biology, Islamic Azad University, Science and Research Branch, Tehran, Iran
4 - Department of Biology, Islamic Azad University, Science and Research Branch, Tehran, Iran
کلید واژه: magnetic field, alfalfa, growth parameters, rhizobial inoculant, physiological indicators,
چکیده مقاله :
Plants are generally subjected to a combination of different conditions such as magnetic field and soil bacteria in their life. The present investigation tried to compare the effects and interactions of magnetic field and rhizobial inoculant in alfalfa. A pot experiment was performed under a natural condition by a factorial design to investigate the influences of magnetic field with 0.75 and 1.5 mT intensities on treated and untreated alfalfa seed with Sinorhizobium meliloti. Results showed that 1.5 mT magnetic intensity reduces growth parameters, protein content, catalase, ascorbate peroxidase, and peroxidase activity as a stress factor while superoxide dismutase activity, malondialdehyde, and reducing sugars content increased. Interaction of rhizobial inoculant and 1.5 mT intensity can reduce the damage caused by magnetic field generated in the plant. Application of 0.75 mT intensity and rhizobial inoculant (individual and together) led to a significant increase in growth parameters, protein content, catalase, ascorbate peroxidase, peroxidase, and superoxide dismutase enzyme activity while malondialdehyde and reducing sugars content reduced. Therefore, as an eco-friendly technique in agriculture, the application of 0.75 mT and rhizobial inoculant might improve the plant quality. This method could be used as a biofertilizer for vegetable production which reduces the environmental pollution caused by the application of biochemical fertilizers.
Adesemoye, A.O., H.A. Torbert and J.W. Kloepper. 2009. 'Plant growth-promoting rhizobacteria allow reduced application rates of chemical fertilizers'. Microbial Ecology, 58: 921-929.
Alemán, E.L., c. Hernández-Aguilar, J.L. González-Olmedo,M.E. González-Vega, Y. Fung-Boix and A.E. Ferrer-Dubois. 2014. 'Effects of EMFs on Some Biological Parameters in Coffee Plants (Coffea arabica L.) obtained by in vitro Propagation'. Polish Journal of Environmental Studies, 23:95-101.
Alikamanoğlu, S., O. Yaycılı, C. Atak and A. Rzakoulieva. 2007. 'Effect of Magnetic Field and Gamma Radiation on Paulowinia tomentosa Tissue Culture'. Biotechnology & Biotechnological Equipment, 21: 49-53.
Alves, B.J.R., R.M. Boddey and S. Urquiaga. 2004. 'The success of BNF in soybean in Brazil'. Plant Soil, 252:1-9.
Asadi Samani, M., L. Pourakbar and N. Azimi. 2013. 'Magnetic field effects on seed germination and activities of some enzymes in cumin'. Life Sciences Journal, 10: 323-328.
Bradford, M.M. 1976. 'A rapid sensitive method for the quantitation of micro program quantities of protein utilizing the principle of protein-dye binding'. Analytical Biochemistry, 72:248-254.
Caverzan, A., A. Casassola and S.P. Bramme. 2016. 'Antioxidant responses of wheat plants under stress'. Genetics and Molecular Biology, 39:1-6.
Giannopolitis, C.N. and S.K. Ries. 1977. 'Superoxide dismutase: I. Occurrence in higher plants'. Plant Physiology, 59:309-314.
Gopalakrishnan, S., A. Sathya, R. Vijayabharathi, R.K. Varshney, C.L.L. Gowda and L. Krishnamurthy. 2015. 'Plant growth promoting rhizobia: challenges and opportunities. Biotechnology Journal, 5: 355-377.
Han, H.S. and K.D. Lee. 2005. 'Plant Growth Promoting Rhizobacteria Effect on Antioxidant Status, Photosynthesis Mineral Uptake and Growth of Lettuce under Soil Salinity'. Research journal of Agriculture and Biological Sciences, 1 (3): 210-215.
Heath, R.L. and L. Packer. 1968. 'Photo peroxidation in isolated chloroplasts. I. Kinetics and stoichiometry of fatty acid peroxidation'. Archives of Biochemistry and Biophysics, 125: 189-98.
Hungria, M., M.A. Nogueira and R.S. Araujo. 2013. 'Co-inoculation of soybeans and common beans with rhizobia and azospirilla: Strategies to improve sustainability'. Biology And Fertility Of Soils, 49:791-801.
Hungria, M., R.J. Campo, E.M. Souza and F.O. Pedrosa. 2010. 'Inoculation with selected strains of Azospirillum brasilense and A. lipoferum improves yields of maize and wheat in Brazil'. Plant Soil, 331:413-425.
Koroi, S.A.A. 1989. 'Gel elektrophers tische and spectral photometrischoe under uchungen zomein fiuss der temperature auf straktur and aktritat der amylase and peroxidase isoenzyme'. Physiology, 20: 15-23.
Lamptey, S., B.D.K. Ahiabor, S. Yeboah and C. Asamoah. 2014. 'Response of soybean (Glycine max) to rhizobial inoculation and phosphorus application'. Journal of Experimental Biology and Agricultural Sciences, 2:72-77.
Maffei, M.E. 2014. 'Magnetic field effects on plant growth, development, and evolution'. Frontiers in plant science, 5:445.
Nakano, Y. and K. Asada. 1981. 'Hydrogen peroxide is scavenged by ascorbate-specific roxidase in spinach chloroplasts'. Plant Cell Physiology, 22:867-880.
Nelson, N. 1944. 'A photometric adaptation of somogi’s method of the determination of glucose'. Journal of Biological Chemistry, 153:375-380.
Pereira, G.J.G., S.M.G. Molina, P.J. Lea and R.A. Azevedo. 2002.'Activity of antioxidant enzyme in response to cadmium in Crotalaria juncea'. Plant Soil, 239:123-132.
Peyvandi, M., N. Kazemi Khaledi and S. Arbabian. 2013. 'The effects of magnetic fields on growth and enzyme activities of Helianthus annuus L. seedlings'. Iranian Journal of Plant Physiology, 3: 717-724.
Priyanka, N. and P. Venkatachalam. 2016. 'Biofabricated zinc oxide nanoparticles coated with phycomolecules as novel micronutrient catalysts for stimulating plant growth of cotton'. Advances in Natural Sciences: Nanoscience and Nanotechnology, 7: 045018.
Radu, F., M. Ahmadi, L. Cojocariu, F. Marian, C. Bostan and A. Borozan. 2010. 'Genotype-biostimulations interactions in some high quality active principles appearance for alfalfa'. Research Journal of Agricultural Sciences,42(1):526-530.
Sahebjamei, H., p. Abdolmaleki and F. Ghanati. 2007. 'Effects of Magnetic Field on the Antioxidant Enzyme Activities of Suspension-Cultured Tobacco Cells'. Bioelectromagnetics, 28:42-47.
Saravanakumar, D., M. Kavino, T. Raguchander, P. Subbian and R. Samiyappan. 2010. 'Plant growth promoting bacteria enhance water stress resistance in green gram plants'. Plant Physiology, 33: 203-209.
Souza, R.d., A. Ambrosini and L. M.P. Passaglia. 2015. 'Plant growth-promoting bacteria as inoculants in agricultural soils'. Genetics and Molecular Biology, 38(4):401-19.
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Stefan, M., N. Munteanu, V. Stoleru and M. Mihasan. 2013. 'Effects of inoculation with plant growth promoting rhizobacteria on photosynthesis, antioxidant status and yield of runner bean'. Romanian Biotechnological Letters, 18(2):8132-8143.
Tairo, E.V. and P.A. Ndakidemi. 2013. 'Possible benefits of rhizobial inoculation and phosphorus supplementation on nutrition, growth and economic sustainability in grain legumes'. American Journal of Research Communication, 1: 532-556.
Vian, A., E. Davies, M. Gendraud and P. Bonnet. 2016. 'Plant Responses to High Frequency Electromagnetic Fields'. BioMed Research International, 2016:1-13.
Yang, J., J.W. Kloepper and C.M. Ryu. 2009. 'Rhizosphere bacteria help plants tolerate abiotic stress'. Trends in Plant Sciences, 14(1):1–4.