The Role of Potassium and Ascorbic acid on Some Growth and Physiological Responses in Catharanthus roseus
محورهای موضوعی : Plant Physiology
Neda Sahi
1
,
Akbar Mostajeran
2
1 - Department of Plant and Animal Biology, Faculty of Biological Science and Technology, University of Isfahan, Isfahan, Iran.
2 - Department of Plant and Animal Biology, Faculty of Biological Science and Technology, University of Isfahan, Isfahan, Iran
کلید واژه: physiological parameters, Ascorbic acid, Catharanthus roseus, K2SO4, KNO3,
چکیده مقاله :
Different concentrations (1.5, 3.16, 15, and 30 mM) and forms (K2SO4 and KNO3) of potassium applied through Hoagland’s nutrient solution. Ascorbic acid in concentrations of 750 and 1500 mg L-1 sprayed twice on seedlings (age 68 and 78 days). Wet and dry weight, plant height, photosynthetic pigments, sodium, and potassium contents measured at the end of the growing season. Also, total free amino acids measured by HPLC. The excess of potassium increased the total wet (62%) and dry weight (54%), leaf area (31%), shoot and root height (49% and 15%, respectively), total chlorophyll (44%), and K+/Na+ ratio (100%), while the total free amino acids (2 times) and sodium content (28%) decreased. Ascorbic acid showed an almost similar trend to potassium in the mentioned factors, but it did not affect the root height and total sodium and potassium contents. There was a positive interaction between the potassium and ascorbic acid on the plant weight and height, leaf area, photosynthetic pigments, and K+/Na+ ratio. Regarding the positive effect of potassium and ascorbic acid on the growth parameters along with the similar changes on the photosynthetic pigments and the K+/Na+ ratio with the reduction of total free amino acids, it may suggest both treatments improved plant growth through plant stability and increasing photosynthetic rate, production of more protein and other metabolites in Catharanthus roseus. Due to the importance of chemical compounds in Catharanthus roseus, any increase in the growth parameters to an increase in the yield of the plant can be very valuable.
Alamri, S. A., M. H. Siddiqui, M. Y. Al-Khaishany, M. Nasir Khan, H. M. Ali, I. A. Alaraidh, A. A. Alsahli, H. Al-Rabiah and M. Mateen, 2018. 'Ascorbic acid improves the tolerance of wheat plants to lead toxicity'. J. Plant Interact. 13(1): 409-419.
Ameer, K., I. Iram, S. Amin, N. Humera, A. Farooq and M. Ibrahim, 2010. 'Alleviation of adverse effects of salt stress in brassica (Brassica campestris) by pre-sowing seed treatment with ascorbic acid'. Am Eurasian J Agric Environ Sci. 7(5): 557-560.
Arafa, A., S. Farouk and H. S. Mohamed, 2011. 'Effect of potassium fertilizer, biostimulants and effective microorganisms as well as their interactions on potato growth, photosynthetic pigments and stem anatomy'. J. Plant Production. 2(8): 1017-1035.Arnon, D. I, 1949. Copper Enzymes in isolated chloroplast polyphenol oxidase in Beta vulgaris. Plant Physiol. 24: 1-5.
Asada, K, 1999. 'The water-water cycle in chloroplasts: scavenging of active oxygens and dissipation of excess photons'. Annual Review of Plant Physiology and Plant Molecular Biology. 50(1): 601-639.
Aziz, A., N. A., Akram and M. A. Ashraf, 2017. ' Influence of natural and synthetic vitamin C (ascorbic acid) on primary and secondary metabolites and associated metabolism in quinoa (Chenopodium quinoa Willd.) plants under water deficit regimes. Plant Physiol. Biochem. 123: 192-203.
Bednarz, C. W, D. M. Oosterhuis and R. D Evans, 1998. 'Leaf photosynthesis and carbon isotope discrimination of cotton in response to potassium deficiency'. Environ Exper Bot. 39(2): 131-139.
Bennett, R. N. and R. M. Wallsgrove, 1994. 'Secondary metabolites in plant defence mechanisms'. new Phytol. 127(4): 617-633.
Besford, R. T, 1978. 'Effect of replacing nutrient potassium by sodium on uptake and distribution of sodium in tomato plants'. Plant Soil. 50: 399-409.
Bhandal, I. S. and C. P. Malik, 1988. 'Potassium estimation, uptake, and its role in the physiology and metabolism of flowering plants'. Int. Rev. Cytol. 110:205-254.
Bybordi, A. 2012. 'Effect of ascorbic acid and silicium on photosynthesis, antioxidant enzyme activity, and fatty acid contents in canola exposure to salt stress'. J. Integr. Agric. 11(10): 1610-1620.
Chakraborty, K., D. Bhaduri, H. N. Meena and K. Kalariya, 2016. 'External potassium (K+) application improves salinity tolerance by promoting Na+-exclusion, K+-accumulation and osmotic adjustment in contrasting peanut cultivars'. Plant Physiol. Biochem. 103: 143-153.
Cheema, M. A., M. Wahid, A. Sattar, F. Rasul and M.F. Saleem, 2012. 'Influence of different levels of potassium on growth, yield and quality of canola (Brassica napus L.) cultivars'. Pak. J. Agric. Sci. 49(2): 163-168.
Chen, Z. and D. R. Gallie, 2004. 'The ascorbic acid redox state controls guard cell signaling and stomatal movement'. Plant Cell. 16(5): 1143-1162.
Clavijo-Sánchez, N., N. Flórez-Velasco and H. Restrepo-Díaz, 2015. 'Potassium nutritional status affects physiological response of tamarillo plants (Cyphomandra betacea Cav.) to drought stress'. J. Agr. Sci. Tech. 17: 1839-1849.
Collin, H. A, 2001. 'Secondary product formation in plant tissue cultures'. Plant Growth Regul. 34(1): 119-134.
Das, S., A. B. Sharangi, 2017. 'Madagascar periwinkle (Catharanthus roseus L.): Diverse medicinal and therapeutic benefits to humankind'. J. Pharmacogn. Phytochem. 6(5): 1695-1701.
De Carolis, E., F. Chan, J. Balsevich and V. De Luca, 1990. 'Isolation and characterization of a 2-oxoglutarate dependent dioxygenase involved in the second-to-last step in vindoline biosynthesis'. Plant Physiol. 94(3): 1323-1329.
Devlin, R. and F. J. D. Witham. 1986. 'Plant physiology,(4thedn.) CBS Publishers and Distributors'. 54.
Domazou, A. S., W. H. Koppenol and J. M. Gebicki, 2009. 'Efficient repair of protein radicals by ascorbate'. Free Radic. Biol. Med. 46(8): 1049-1057.
Egilla, J. N., F. T. Davies and M. C. Drew, 2001. 'Effect of potassium on drought resistance of Hibiscus rosa-sinensis cv. Leprechaun: Plant growth, leaf macro-and micronutrient content and root longevity'. Plant Soil. 229(2): 213-224.
Ei-Tohamy, W. A., H. M. Ei-Abagy and N. H. M. Ei-Greadly, 2008. Studies on the effect of putrescine, yeast and vitamin C on growth, yield and physiological responses of eggplant (Solanum melongena L.) under sandy soil conditions. Aust. J. Basic & Appl. Sci. 2(2): 296-300.
Ejaz, B., Z. A. Sajid and F. Aftab, 2012. Effect of exogenous application of ascorbic acid on antioxidant enzyme activities, proline contents, and growth parameters of Saccharum spp. hybrid cv. HSF-240 under salt stress. Turk J Biol. 36(6): 630-640.
El-Morsy, A.H., A.S. Ezzat and U.M. El-Deen, 2010. 'Effect of some phosphorus and potassium rates and foliar spray with antioxidants on growth, yield and yield quality of garlic (Allium sativum L.)'. Annals Of Agric.Sc. 48(3): 27-40.
El-Seifi, S. K., M. A. Hassan, S. M. H. Serg, U. M. S. El-Deen and M. A. Mohamed, 2014. 'Effect of calcium, potassium and some antioxidants on growth, yield and storability of sweet potato'. Annals of Agric. Sci. 52(1).
Fayez, K. A. and S. A. Bazaid, 2014. 'Improving drought and salinity tolerance in barley by application of salicylic acid and potassium nitrate'. J. Saudi Soc. 13(1): 45-55.
Flowers, T. J., R. Munns and T. D. Colmer, 2015. 'Sodium chloride toxicity and the cellular basis of salt tolerance in halophytes'. Ann Bot. 115(3): 419-431.
Gallie, D. R, 2013. 'L-ascorbic acid: a multifunctional molecule supporting plant growth and development'. Scientifica. 2013: 1-24.
Ganie, A. H., A. Ahmad, P. Y. Yousuf, R. Pandey, S. Ahmad, I. M. Aref and M. Iqbal, 2017. 'Nitrogen-regulated changes in total amino acid profile of maize genotypes having contrasting response to nitrogen deficit'. Protoplasma. 254(6): 2143-2153.
Gebicki, J. M., T. Nauser, A. Domazou, D. Steinmann, P. L. Bounds and W. H. Koppenol, 2010. 'Reduction of protein radicals by GSH and ascorbate: potential biological significance'. Amino Acids. 39(5): 1131-1137.
Gerardeaux, E., E. Saur, J. Constantin, A. Porté and L. Jordan-Meille, 2009. 'Effect of carbon assimilation on dry weight production and partitioning during vegetative growth'. Plant Soil. 324(1-2): 329-343.
Ghorbanli, M., M. Gafarabad, T. Amirkian and B. Allahverdi, 2012. ' Investigation of proline, total protein, chlorophyll, ascorbate and dehydroascorbate changes under drought stress in Akria and Mobil tomato cultivars. Iran. J. Plant Physiol. 3 (2): 651-658.
Guo, J., Y. Jia, H. Chen, L. Zhang, J. Yang, J. Zhang, X. Hu, X. Ye, Y. Li and Y. Zhou, 2019. 'Growth, photosynthesis, and nutrient uptake in wheat are affected by differences in nitrogen levels and forms and potassium supply'. Sci Rep. 9(1): 1-12.
Hasanuzzaman, M., M. Bhuyan, K. Nahar, M. Hossain, J. A. Mahmud, M. Hossen, A. A. C. Masud and M. Fujita, 2018. 'Potassium: A vital regulator of plant responses and tolerance to abiotic stresses'. Agronomy. 8(3): 31.
Helal, H. and K. Mengel, 1979. 'Nitrogen metabolism of young barley plants as affected by NaCl-salinity and potassium'. Plant Soil. 51(4): 457-462.
Herrera-Martínez, S.L., M. Mora-Herrera, R. García-Velasco, J. Gomora-Rasso and G. Rogel-Millán, 2013. Effect of ascorbic acid on growth, photosynthetic pigments and peroxidase activity of rosebush'. Terra Latinoamericana. 31(3): 193-199.
Herrera-Rodríguez, M. B., R. Pérez-Vicente and J. M. Maldonado, 2007. 'Expression of asparagine synthetase genes in sunflower (Helianthus annuus) under various environmental stresses'. Plant Physiol Biochem. 45(1): 33-38.
Hildebrandt, T. M., A. N. Nesi, W. L. Araújo and H. P. Braun, 2015. 'Amino acid catabolism in plants'. Mol Plant. 8(11): 1563-1579.
Hsiao, T. C., R. Hageman and E. H. Tyner, 1970. 'Effects of Potassium Nutrition on Protein and Total Free Amino Acids in Zea mays'. Crop Sci. 10(1): 78-82.
Hu, W., T. D. Coomer, D. A. Loka, D. M. Oosterhuis and Z. Zhou, 2017. 'Potassium deficiency affects the carbon-nitrogen balance in cotton leaves'. Plant Physiol. Biochem. 115: 408-417.
Hu, W., W. Zhao, J. Yang, D. M. Oosterhuis, D. A. Loka and Z. Zhou, 2016. 'Relationship between potassium fertilization and nitrogen metabolism in the leaf subtending the cotton (Gossypium hirsutum L.) boll during the boll development stage'. Plant Physiol. Biochem. 101: 113-123.
Huda, A. I., U. El-Behairy, M. El-Desuki, M. Bakry and A. F. Abou-Hadid, 2010. 'Response of green bean to fertilization with potassium and magnesium'. Res. j. agric. biol. sci. 6(6): 834-839.
Hussain, F., A. Malik, M. A. Haji and A. L. Malghani, 2011. 'Growth and yield response of two cultivars of mungbean (Vigna radiata L.) to different potassium levels'. J. Anim. Plant Sci. 21(3): 622-625.
Hussein, M. M. and A. K. Alva, 2014. 'Effects of zinc and ascorbic acid application on the growth and photosynthetic pigments of millet plants grown under different salinity'. Agric. Sci. 5(13): 1253.
Hussein, M. M., S. Y. El-Faham and A. K Alva, 2012. 'Pepper plants growth, yield, photosynthetic pigments, and total phenols as affected by foliar application of potassium under different salinity irrigation water'. Agric. Sci. 2: 1-8.
Iqbal, A. and Z. Hidayat, 2016. 'Potassium management for improving growth and grain yield of maize (Zea mays L.) under moisture stress condition'. Sci Rep. 6: 34627.
Islam, M. S., M. M. Haque, M. M. Khan, T. hidaka and M. A. Karim, 2004. 'Effect of fertilizer potassium on growth, yield and water relations of bushbean (Phaseolus vulgaris L.) under water stress conditions'. Jpn. J. Trop. Agr. 48(1): 1-9.
Jiang, C.-c., C. Fang, X.-Z. Gao, J.-w. Lu, K.-y. Wan, F. Z. Nian and Y. Wang, 2008. 'Study on the nutrition characteristics of different K use efficiency cotton genotypes to K deficiency stress'. Agr Sci China. 7(6): 740-745.
Jin, S. H., J. Q. Huang, X. Q. Li, B. S. Zheng, J. S. Wu, Z. J. Wang, G. H. Liu and M. Chen, 2011. 'Effects of potassium supply on limitations of photosynthesis by mesophyll diffusion conductance in Carya cathayensis'. Tree Physiol. 31(10): 1142-1151.
Jung, S, 2004. 'Variation in antioxidant metabolism of young and mature leaves of Arabidopsis thaliana subjected to drought'. Plant Sci. 166(2): 459-466.
Khan, G. A. and M. Sajid, 2007. 'Response of dhalia (Dhalia pinnata) to different levels of nitrogen alone and in combination with constant doses of phosphorus and potassium'. Am.-Eurasian J. Sustain. Agric. 1: 25-32.
Khan, T., M. Mazid and F. Mohammad, 2011. 'A review of ascorbic acid potentialities against oxidative stress induced in plants'. J. Agrobiol. 28(2): 97-111.
Koch, K., K. Mengel, 1974. 'The influence of the level of potassium supply to young tobacco plants (Nicotiana tabacum L.) on short‐term uptake and utilisation of nitrate nitrogen (15N). J. Sci. Food Agric. 25(5): 465-471.
Kovács, Z., L. Simon-Sarkadi, I. Vashegyi and G. Kocsy, 2012. 'Different accumulation of free amino acids during short-and long-term osmotic stress in wheat. Sci. World J. 2012: 1-10.
Kwizera, C., B. T. I. Ong'or, S. Kaboneka, F. Nkeshimana and N. Ahiboneye, 2019. 'Effects of Potassium Fertilizer on Bean growth and Yield parameters'. I. J. of Advances in Scientific Research and Engineering. 5: 1-7.
Lindhauer, M. G, 1985. 'Influence of K nutrition and drought on water relations and growth of sunflower (Helianthus annuus L.)'. J. Plant. Nutr. 148(6): 654-669.
MacCarthy, J. J., D. Ratcliffe and H. E. B. Street, 1980. 'The effect of nutrient medium composition on the growth cycle of Catharanthus roseus G. Don cells grown in batch culture'. J. Exp. Bot. 31(5): 1315-1325.
Makhdum, M.I., H. Pervez and M. Ashraf, 2007. 'Dry matter accumulation and partitioning in cotton (Gossypium hirsutum L.) as influenced by potassium fertilization'. Biol Fert Soils. 43(3): 295-301.
Marschner, P. 2012. 'Marschner, s mineral nutrition of higher plants., 3rd edn (Academic Press: London)'.
Min, K., K. Chen and R. Arora, 2020. 'A metabolomics study of ascorbic acid‐induced in situ freezing tolerance in spinach (Spinacia oleracea L.)'. Plant Direct. 4(2): 1-13.
Moreno, P. R., R. van der Heijden and R. Verpoorte, 1995. 'Cell and tissue cultures of Catharanthus roseus: a literature survey'. Plant Cell, Tissue Organ Cult. 42(1): 1-25.
Nagaveni, H. C., C. B. Kumari and A. M. Suma, 2018. 'Effect of different nutrient levels on growth and yield of some medicinal plants'. J. Pharmacogn. Phytochem. 3: 110-113.
Naz, H., N. A. Akram and M. Ashraf, 2016. 'Impact of ascorbic acid on growth and some physiological attributes of cucumber (Cucumis sativus) plants under water-deficit conditions'. Pak. J. Bot. 48(3): 877-883.
Nisar, A., A. S. Mamat, M. I. Hatim, M. S. Aslam and M. Syarhabil, 2016. 'An updated review on Catharanthus roseus: phytochemical and pharmacological analysis'. Indian J. Pharm. Sci. 3(2): 631-653.
Noctor, G. and C. H. Foyer, 1998. 'Ascorbate and glutathione: keeping active oxygen under control'. Annu Rev Plant Physiol Plant Mol Biol. 49(1): 249-279.
Obata, T. and A. R. Fernie, 2012. 'The use of metabolomics to dissect plant responses to abiotic stresses'. Cell. Mol. Life Sci. 69(19): 3225-3243.
Ortas, I, 2018. 'Influence of potassium and magnesium fertilizer application on the yield and nutrient accumulation of maize genotypes under field conditions'. J. Plant Nutr. 41(3): 330-339.
Pettigrew, W. T, 2008. 'Potassium influences on yield and quality production for maize, wheat, soybean and cotton'. Physiol. Plant. 133(4): 670-681.
Pi, Z., P. Stevanato, L. Yv, G. Geng, X. L. Guo, Y. Yang, C. X. Peng and X. S. Kong, 2014. 'Effects of potassium deficiency and replacement of potassium by sodium on sugar beet plants'. Russ. J. Plant Physiol. 61(2): 224-230.
Prajapati, K. and H. A. Modi, 2012. 'The importance of potassium in plant growth-a review'. Indian J. Plant Physiol. 1(02): 177-186.
Römheld, V. and E. A. Kirkby, 2010. 'Research on potassium in agriculture: needs and prospects'. Plant Soil. 335(1-2): 155-180.
Salmanizadeh, H. and N. Sahi, 2020. Determination of amino acid profile for argininosuccinic aciduria disorder using High-Performance Liquid Chromatography with fluorescence detection. Acta Biochim. Pol. 67: 347-351.
Shafeek, M. R., O. A. H. El-Zeiny and M. E. Ahmed, 2005. 'Effect of natural phosphate and potassium fertilizer on growth, yield and seed composition of pea plant in new reclaimed soil'. Asian J. Plant Sci. 4: 608-612.
Sideris, C. P. and H. Y. Young, 1945. 'Effects of potassium on chlorophyll, acidity, ascorbic acid, and carbohydrates of Ananas comosus (L.) Merr'. Plant Physiol. 20(4): 649.
Silva, M. d. A., J. L. Jifon, J. A. Da Silva and V. Sharma, 2007. 'Use of physiological parameters as fast tools to screen for drought tolerance in sugarcane'. Braz. J. Plant Physiol. 19(3): 193-201.
Singh, N. and R. D. Bhardwaj, 2016. ' Ascorbic acid alleviates water deficit induced growth inhibition in wheat seedlings by modulating levels of endogenous antioxidants'. Biologia. 71(4): 402-413.
Smirnoff, N, 1996. 'Botanical briefing: the function and metabolism of ascorbic acid in plants'. Ann. Bot. 78(6): 661-669.
Stadtman, E. R. and C. N. Oliver, 1991. 'Metal-catalyzed oxidation of proteins. Physiological consequences'. J. Biol. Chem. 266(4): 2005-2008.
Tanaka, A. and A. Makino, 2009. 'Photosynthetic research in plant science'. Plant Cell Physiol. 50(4): 681-683.
Tang, Z.-H., A.-J. Zhang, M. Wei, X.-G. Chen, Z.-H. Liu, H.-M. Li and Y.-F. Ding, 2015. 'Physiological response to potassium deficiency in three sweet potato (Ipomoea batatas [L.] Lam.) genotypes differing in potassium utilization efficiency'. Acta Physiol. Plant. 37(8): 184.
Venkatesh, J. and S. W. Park, 2014. 'Role of L-ascorbate in alleviating abiotic stresses in crop plants'. Bot Stud. 55(1): 38.
Verpoorte, R., R. van der Heijden and P. R. Moreno. 1997. Biosynthesis of terpenoid indole alkaloids in Catharanthus roseus cells. The Alkaloids: Chemistry and Pharmacology. Elsevier. 49:221-299.
Verpoorte, R., R. Van der Heijden, H. J. G. Ten Hoopen and J. Memelink, 1999. 'Metabolic engineering of plant secondary metabolite pathways for the production of fine chemicals'. Biotechnol. Lett. 21(6): 467-479.
Wakeel, A., M. Farooq, M. Qadir and S. Schubert, 2011. 'Potassium substitution by sodium in plants'. CRC Crit Rev Plant Sci. 30(4): 401-413.
Wall, M. E, 1940. 'The role of potassium in plants: II. Effect of varying amounts of potassium on the growth status and metabolism of tomato plants'. Soil Sci. 49(4): 315.
Wang, H., D. Liu, J. Sun and A. Zhang, 2005. 'Asparagine synthetase gene TaASN1 from wheat is up-regulated by salt stress, osmotic stress and ABA'. J. Plant Physiol. 162(1): 81-89.
Wang, M., Q. Zheng, Q. Shen and S. Guo, 2013. 'The critical role of potassium in plant stress response'. Int. J. Mol. Sci. 14(4): 7370-7390.
Wang, X.-g., X.-h. Zhao, C.-j. Jiang, C.-h. Li, C. Shan, W. Di, Y.-q. Chen, H.-q. YU and C.-y. Wang, 2015. 'Effects of potassium deficiency on photosynthesis and photoprotection mechanisms in soybean (Glycine max (L.) Merr.)'. J. Integr. Agric. 14(5): 856-863.
Zelelew, D. Z., S. Lal, T. T. Kidane and B. M. Ghebreslassie, 2016. 'Effect of potassium levels on growth and productivity of potato varieties'. J. Sci. Res. 7(12): 1629-1638.
Zhang, Q. L., Y. X. Wei and C. L. Peng, 2018. 'Effects of endogenous ascorbic acid on resistance to high-temperature stress in excised rice leaves'. Photosynthetica. 56(4): 1453-1458.
Zhao, D., D. M. Oosterhuis and C. W. Bednarz, 2001. 'Influence of potassium deficiency on photosynthesis, chlorophyll content, and chloroplast ultrastructure of cotton plants'. Photosynthetica. 39(1): 103-109.
Zonouri, M., T. Javadi, N. Ghaderi and M. K. Saba, 2014. 'Effect of foliar spraying of ascorbic acid on chlorophyll a, chlorophyll b, total chlorophyll, carotenoids, hydrogen peroxide, leaf temperature and leaf relative water content under drought stress in grapes'. Bull Environ Pharmacol Life Sci. 3: 178-184.
