Stigmasterol alleviates the impacts of drought in flax and improves oil yield via modulating efficient antioxidant and ROS homeostasis
محورهای موضوعی : Plant Physiology
Mamdouh Nemat Alla
1
,
Nemat Hassan
2
,
Inas Budran
3
,
Zeinab El-Bastawisy
4
,
Ebtisam El-Harary
5
1 - Botany Department, Faculty of Science, Damietta University, Damietta, Egypt
2 - Botany Department, Faculty of Science, Damietta University, Damietta, Egypt
3 - Botany Department, Faculty of Science, Damietta University, Damietta, Egypt
4 - Botany Department, Faculty of Science, Damietta University, Damietta, Egypt
5 - El-Zawia University, Faculty of Science, Botany Department, Libya
کلید واژه: Water stress, Tolerance, linseed, Fatty Acid Composition, Free radicles,
چکیده مقاله :
The present study aimed at alleviating the impacts of drought on flax growth, antioxidants and ROS homeostasis by stigmasterol. Seeds were soaked in water or stigmasterol and sown in plastic pots. On the 24th day after sowing (DAS), water regime was applied and samples were harvested up to the 56th DAS for measuring growth parameters, free radicles, antioxidants, and POD and Rubisco quantification. At seed maturity, yield analysis measurements (capsules, seeds, oil yield and fatty acid composition) were performed. Drought provoked significant decreases in growth parameters, ascorbic acid and glutathione but elevated lipid peroxidation and H2O2 concurrently with significant inhibition in the activities of catalase, guiacol peroxidase, ascorbic peroxidase and glutathione reductase as well as activity and quantification of peroxidase and ribulose 1,5-bisphosphate carboxylase/oxygenase (Rubisco). Yield analysis demonstrated decreases in capsule and seed numbers, the oil and fatty acid contents and the fatty acid composition. Nevertheless, stigmasterol mitigated the drastic effects of drought on growth parameters, antioxidants and Rubisco and rendered the contents of lipid peroxides and H2O2 comparable to control. In the meantime, oil yield and fatty acid composition were improved in synchronization with the efficiency of antioxidants and ROS homeostasis. These findings conclude that drought resulted in a state of stress in flax; however, stigmasterol alleviated these drastic impacts and improved oil yield and fatty acid composition via modulating efficient antioxidant capacity and ROS homeostasis.
Aebi, H. 1984. Catalase in vitro. Methods Enzymol, 105: 121-126.
Ahn, Y.O., S.Y. Kwon, H.S. Lee, I.H. Park and S.S. Wak. 1999. Biosynthesis and metabolism of vitamin C in suspension cultures Scutellaria baicalensis. J Biochem Mol Biol, 32: 451-455.
Alexieva, V., I. Sergiev, S. Mapelli and E. Karanov. 2001. The effect of drought and ultraviolet radiation on growth and stress markers in pea and wheat. Plant Cell Environ, 24, 1337-1344.
Anderson, M.P. and J.W. Gronwalds. 1991. Atrazine resistance in velvetleaf (Abutilon theophrasti) biotype due to enhanced glutathione S-transferase activity. Plant Physiol, 96: 107–109.
A.O.A.C. 1975. Official methods of analysis. Assoc. Off .Agric. Chem. Virginia, USA.
Aravind, P. and M.N. Prasad. 2005. Modulation of cadmium-induced oxidative stress in Ceratophyllum demersum by zinc involves ascorbate–glutathione cycle and glutathione metabolism. Plant Physiol Biochem, 43: 107-116.
Berni, R., M. Luyckx, X. Xu, S. Legay, K. Sergeant, J. Hausman, S. Lutts, G. Cai and G. Guerriero. 2019. Reactive oxygen species and heavy metal stress in plants: Impact on the cell wall and secondary metabolism. Environ Exp Bot, 161: 98-106.
Blokhina, O., E. Virolainen and K.V. Fagerstedt. 2003. Antioxidants, oxidative damage and oxygen deprivation stress: A review. Ann Bot, 91, 179–194.
Chance, B. and A.C. Maehly. 1955. Assay of catalase and peroxidase. Methods Enzymol, 2: 764-775.
Danish, M. and M. Nizami. 2019. Complete fatty acid analysis data of flaxseed oil using GC-FID method. Data brief, 23: 103845.
Feller, U., I. Anders and T. Mae. 2008. Rubiscolytics: fate of Rubisco after its enzymatic function in a cell is terminated. J Exp Bot, 59: 1615-1624.
Foyer, C.H., F.L. Theodoulou and S. Delrot. 2001. The function of inter- and intracellular glutathione transport systems in plants. Trends Plant Sci, 4: 486-492.
Ghasemlou, F., H. Amiri, R. Karamian, A. Mirzaie-asl. 2019. Alleviation of the effects of on drought stress Verbascum nudicuale by methyl jasmonate and titanium dioxide nanoparticles. Iran J Plant Physiol, 9: 2911-2920.
Hartmann, M.A. 2009. Plant sterols and the membrane environment. Trends Plant Sci, 3: 170–175.
Hassan, N.M., M.S. Serag, F.M. El-Feky and M.M. Nemat Alla. 2008. In vitro selection of mung bean and tomato for improving tolerance to NaCl. Ann Appl Biol, 152: 319-330.
He, J.X., S. Fujioka and T.C. Li. 2003. Sterols regulate development and gene expression in Arabidopsis. Plant Physiol, 131: 1258-1269.
Heath, R.L. and L. Packer. 1968. Photoperoxidation in isolated chloroplasts. 1. Kinetics and stoichiometry of fatty acid peroxidation. Arch Biochem Biophys, 125: 189-198.
Johnson, M. and C. Bradford. 2014. Omega-3, omega-6 and omega-9 fatty acids: Implications for cardiovascular and other diseases. J Glycomics Lipidomics 4: 123-132.
Laemmlli, E.K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227: 680-685.
Lee, R., J. Hench and G. Ruvkun. 2001. Regulation of C. elegans DAF-16 and its human ortholog FKHRL1 by the daf-2 insulin-like signaling pathway. Curr Biol, 11: 1950–1957.
Mittler, R. 2002. Oxidative stress, antioxidants and stress tolerance. Trends Plant Sci, 7: 405–410.
Nakano, Y. and K. Asada. 1981. Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts. Plant Cell Physiol, 22: 867-880.
Nayyar, H. and D. Gupta. 2006. Differential sensitivity of C3 and C4 plants to water deficit stress: Association with oxidative stress and antioxidants. Environ Exp Bot, 58: 106-113.
Nemat Alla, M.M., N.M. Hassan and Z.M. El-Bastawisy. 2008. Changes in antioxidants and kinetics of glutathione-S-transferase of maize in response to isoproturon treatment. Plant Biosystems, 142: 5-16.
Nemat Alla, M.M., G.M. Abogadallah, E.G. Badran and R.M. Nada. 2014. Differential tolerance of two wheat cultivars to NaCl is related to antioxidant potentialities. Braz J Bot, 37: 207–215.
Perveen, S., N. Iqbal, M. Saeed, S. Zafar and Z. Arshad. 2018. Role of foliar application of sulfur-containing compounds on maize (Zea mays L. var. Malka and hybrid DTC) under salt stress. Braz J Bot, 41: 805–815.
Quiles, M.J. and N.I. Lopez. 2004. Photoinhibition of photosystems I and II induced by exposure to high light intensity during oat plant growth: Effects on the chloroplast NADH dehydrogenase complex. Plant Sci, 166: 815-823.
Rao, S.R., B.V. Vardhini, E. Sujatha and S. Anuradha. 2002. Brassinosteroids a new class of plant phytohormones. Curr Sci, 82: 1239-1244.
Razavizadeh R. and M. Karami. 2018. Antioxidant capacity and chemical composition of Carum copticum under PEG treatment. Iran J Plant Physiol, 8: 2321-2331.
Schaedle, M. and J.A. Bassham. 1977. Chloroplast glutathione reductase. Plant Physiol, 59: 1011-1012.
Seevers, P.M., J.M. Daly and F.F. Catedral. 1971. The role of peroxidase isozymes in resistance to wheat stem rust disease. Plant Physiol, 48: 353–360.
Shao, H.B., L.Y. Chu, M.A. Shao, C. Abdul Jaleel and M. Hong-Mei. 2008. Higher plant antioxidants and redox signaling under environmental stresses. Comp Rend Biol, 331: 433–441.
Taylor, N.L., C. Rudhe, J.M. Hulett, T. Lithgow, E. Glaser, D.A. Day, A.H. Millar and J. Whelan. 2003. Environmental stresses inhibit and stimulate different protein import pathways in plant mitochondria. FEBS Lett, 547: 125–130.
Thoenen, M., B. Herrmann and U. Feller. 2007. Senescence in wheat leaves: Is a cysteine endopeptidase involved in the degradation of the large subunit of Rubisco? Acta Physiol Plant, 29: 339-350.
Yamazaki, J., A. Ohashi, Y. Hashimoto, E. Negishi, S. Kumagai, T. Kubo, T. Oikawa, E. Maruta and Y. Kamimura. 2003. Effects of high light and low temperature during harsh winter on needle photodamage of Abies mariesii growing at the forest limit on Mt. Norikura in Central Japan. Plant Sci, 165: 257–264.
