Physiological Changes During Cold Storage of Lilium ledebourii (Baker) Boiss. Bulb, a Rare Indigenous Species to Iran
محورهای موضوعی : مجله گیاهان زینتیFatemeh Abazarifard 1 , Seyed Najmmaddin Mortazavi 2 , Rohangiz Naderi 3 , Sepideh Kalateh Jari 4
1 - Department of Horticultural Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran
2 - Department of Horticultural Sciences, Faculty of Agriculture, University of Zanjan, Zanjan, Iran
3 - Department of Horticultural Sciences, Faculty of Agricultural Science and Engineering, University of Tehran, Tehran, Iran
4 - Department of Horticultural Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran
کلید واژه: Hormone, Saponin, Lily, Soluble sugars, Cold period,
چکیده مقاله :
Lilium ledebourii is a native and endangered rare species in Iran. It requires a cold period for proper growth and flowering. This experiment was performed to investigate the effects of cold storage on changes of endogenous abscisic acid (ABA), indole-3-acetic acid (IAA), gibberellic acid (GA3), sucrose, fructose, glucose, and total saponin during dormancy of Lilium ledebourii bulbs. The bulbs of lily were stored at 1 or 7 ºC, for 0, 30, 60, 90, and 120 days, and after each period removed from storage and analyzed using HPLC system. The results indicated that, cold storage at 1 ºC for 120 days decreased the levels of ABA while, 7 ºC in the same duration increased its content, slightly. The levels of IAA increased at 1 ºC storage, while remained almost constant at 7 ºC. Cold treatment at 1 ºC increased the levels of GA3, while storage at 7 ºC caused slight decrease in its content. Sucrose and fructose concentrations increased in both temperatures during the storage, while minor reduction in glucose content was observed. Storage at 7 ºC for 120 days induced the highest content of total saponin. The magnitude of changes in hormones and soluble sugars measured at 1 ºC was greater than that at 7 ºC.
سوسن چلچراغ یکی از گونههای نادر بومی و در معرض خطر انقراض است که برای رشد و گلدهی مناسب نیاز به یک دوره سرمایی دارد. این آزمایش بهمنظور بررسی اثرات سرمادهی پیاز بر تغییرات تنظیم کنندههای رشد درونزای آبسیزیک اسید (ABA)، ایندول استیک اسید (IAA)، جیبرلیک اسید (GA3) و قندهای محلول ساکارز، فروکتوز، گلوکز و همچنین ساپونین تام در طول خواب پیاز سوسن چلچراغ انجام شد. بدینمنظور پیازهای این گیاه در دمای 1 یا 7 درجه سانتیگراد به مدت 0، 30، 90،60 و 120 روز قرار گرفتند و پس از پایان هر دوره، از انبار برداشت و توسط دستگاه HPLC تجزیه و تحلیل شدند. نتایج نشان داد که سرمادهی پیازها در یک درجه سانتیگراد به مدت 120 روز باعث کاهش سطح ABA شد در حالیکه 7 درجه سانتیگراد در همان مدت زمان سبب افزایش جزیی در سطح این هورمون شد. تیمارهای سرمایی یک درجه سانتیگراد سبب افزایش سطح IAA و GA3 شدند اما در 7 درجه سانتیگراد سطح IAA تقریبا ثابت باقی ماند و GA3 کاهش جزئی نشان داد. غلظتهای ساکارز و فروکتوز در هر دو تیمار دمایی افزایش یافتند در حالیکه مقدار گلوکز کاهش یافت. انبار پیازها در 7 درجه سانتیگراد به مدت 120 روز بالاترین سطح ساپونین تام را سبب شد. تغییرات هورمونها و قندهای اندازه گیری شده در 1 درجه سانتیگراد بیشتر از 7 درجه سانتیگراد بود.
Aguettaz, P., Paffen, A., Delvallee, I., Linde, P.V.D. and Klerk, G.J.D. 1990. The development of dormancy in bulblet of Lilium speciosum generated in vitro the effect of culture conditions. Plant Cell, Tissue and Organ Culture, 22: 167-172.
An, L., Xie, Z., Wang, M., An, L., Pu, C., Li, Y., Zhang, Y., Wu, L. and Zhao, L. 2010. Variation in endogenous concentration of abscisic acid, gibberellic acid, zeatin and 3-indole acetic acid during cold storage in bulbs of lily (Lilium ‘Siberia’). Philippine Agricultural Scientist, 93: 281-290.
Aung, L.H. and Hertogh, A.A.D. 1967. The occurrence of gibberellin-like substances in tulip bulbs (Tulipa sp.). Plant Cell Physiology, 8: 201-205.
Chen, Q.B., Hou, X.L., Wang, J.J. and Han, J.M. 2007. Physiological and biochemical changes of onion with different maturities during the dormancy stage. Acta Horticultura Sinica, 34 (1): 222-224.
Dole, J. M. 2003. Research approaches for determining cold requirements for forcing and flowering of geophytes. HortScience, 38 (3): 341-346.
Farsam, H., Amanlou, M., Amin, G., Nezamivand – Chegini, G., Salehi- Surmaghi, M. and Shafiee, A. 2003. Anatomical and phytochemical study of Lilium ledebourii (Baker) Boiss., a rare endemic species in Iran. DARU, 11 (4): 164-170.
Gu, J., Zeng, Z., Wang, Y. and Lyu, Y. 2020. Transcriptome analysis of carbohydrate metabolism genes and molecular regulation of sucrose transport gene LoSUT on the flowering process of developing oriental hybrid lily ‘Sorbonne’ bulb. International Journal of Molecular Science, 21: 3092-3119.
Hartmann, H.T., Kester, D.E. and Davies, F.T. 1990. Plant propagation, principles and practices. Fifth ed., Prentice-Hall International Inc., Englewood Cliffs, New Jersey.
Hou, S., Zhu, J., Ding, M. and Lv, G. 2008. Simultaneous determination of gibberellic acid, indole-3- acetic acid, and abscisic acid in wheat extracts by solid-phase extraction and liquid chromatography- electrospray tandem mass spectrometry. Talanta, 76 (4): 798-802.
Kamenetsky, R. and Okubo, H. 2013. Ornamental geophytes: From basic science to sustainable production. CRC Press Taylor and Francis Group. Boca Raton. FL 33487-2742. USA: P 578.
Khodorova, N.V. and Boitel – Conti, M. 2013. The role of temperature in the growth and flowering of geophytes. Plants, 2: 699-711.
Kilsdonk, M.G.V. 2002. Assessment of the internal quality of stored flower bulbs using magnetic resonance imaging. Utrecht University. Retrieved from http://dspace.library.uu.nl/bitstream/handle/1874/1771
Kim, K.J. and Kim, K.S. 2005. Changes of endogenous growth substances during bulb maturation after flowering in Lilium oriental hybrid ‘Casa Blanca’. Acta Horticulturae, 673: 661-667.
Langens – Gerrits, M.M., Miller, W.B.M., Croes, A.F. and Klerk, G.J.D. 2003. Effect of low temperature on dormancy breaking and growth after planting in lily bulblets regenerated in vitro. Plant Growth Regulation, 40: 267-275.
Li, X.J., Yang, M.F., Chen, H., Qu, L.Q., Chen, F. and Shen, S.H. 2010. Abscisic acid pretreatment enhances salt tolerance of rice seedling: Proteomic evidence. Biochimica et Biophysica Acta, 1804: 929-940.
Liu, X., Wang, Q., Gu, J. and Lu, Y. 2014. Vernalization of oriental hybrid lily ‘Sorbonne’: Changes in physiology activity and molecular mechanism. Molecular Biology Reports, 41: 6619-6634.
Ma, Z., Ge, L., Lee, A. S. Y., Yong, J. W. H., Tan, S. N. and Ong, E. S. 2008. Simultaneous analysis of different classes of phytohormones in coconut (Cocos nucifera L.) water using high-performance liquid chromatography and liquid chromatography- tandem mass spectrometry after solid-phase extraction. Analytica Chimica Acta, 610: 274-281.
Miller, W.B. and Langhans, R.W. 1990. Low temperature alters carbohydrate metabolism in Easter lily bulbs. HortScience, 25 (4): 463-465.
Mirzakhani, A. 2010. The investigation of some physiological changes during vernalization and scale propagation of Lilium LA hybrid and chelcheragh lily, Lilium ledebourii. Ph.D. Thesis in Horticulture. Tehran University.
Mornya, P.M.P. and Cheng, F. 2013. Seasonal changes in endogenous hormone and sugar contents during bud dormancy in tree peony. Journal of Applied Horticulture, 15 (3): 159-165.
Ncube, B., Finnie, J.F. and van Staden, J. 2011. Seasonal variation in antimicrobial and phytochemical properties of frequently used medicinal bulbous plants from South Africa. South African Journal of Botany, 77: 387-396.
Nie, L.C., Chen, Y.H. and Liu, M. 2016. Effects of low temperature and chilling duration on bud break and changes of endogenous hormones of asparagus. European Journal of Horticultural Science, 81 (1): 22-26.
Nikolic, M., Misic, D., Maksimovic, V., Jevremovic, S., Trifunovic, M. and Subotic, A. 2008. Effect of low temperature on rooting rate and carbohydrate content of Fritillaria meleagris bulbs formed in culture in vitro. Archives of Biological Science, 60 (1): 5-6.
Okubo, H., Chijiwa, M. and Uemoto, S. 1988. Seasonal changes in leaf emergence from scale bulblets during scaling and endogenous plant hormone levels in Easter lily (Lilium longiflorum Thunb). Journal of the Faculty of Agriculture Kyushu University, 33 (1.2): 9-15.
Padasht Dehkaei, M.N. 2004. Study methods of culture and propagation of chel- cheragh lily plant native to Iran for introduce to new ornamental crop. Ph.D. thesis in horticulture. Science and Research Branch of Islamic Azad University .186p.
Park, Y.B. and Lee, B.Y. 1992. Effect of storage temperature on change in carbohydrate and endogenous hormones in garlic bulbs. Journal of the Korean Society for Horticultural Science, 33: 442-451.
Podwyszynska, M. 2012. The mechanisms of in vitro storage organ formation in ornamental geophytes. Floriculture and Ornamental Biotechnology, 6 (1): 9-23.
Rebers, M., Vermeer, E., Knegt, E. and van der Plas, L.H.W. 1996. Gibberellin levels are not a suitable indicator for properly cold–treated tulip bulbs. HortScience, 31 (5): 837-838.
Roh, M.S. 1996. New production technology of Lilium- a review on propagation and forcing. Acta Horticulturae, 414: 219-223.
Sharma, K., Lee, Y.R., Park, S.W. and Nile, S.H. 2016. Importance of growth hormones and temperature for physiological regulation of dormancy and sprouting in onions. Food Reviews International, 32: 233-255.
Shibasaki, K., Uemura, M., Tsurumi, S. and Rahman, A. 2009. Auxin response in Arabidopsis under cold stress: Underlying molecular mechanisms. The Plant Cell, 21: 3823-3838.
Shin, K.S., Chakrabarty, D. and Peak, K.Y. 2002. Sprouting rate, change of carbohydrate contents and related enzymes during cold treatment of lily bulblets regenerated in vitro. Scientia Horticulturae, 96: 195-204.
Sun, H.M., Li, T.L. and Li, Y.F. 2005. Physiological mechanism of metabolism of carbohydrate, phenols, free amino acid, and endogenous hormones in middle scales of Lilium davidii var. Unicolor bulbs stored at low temperature for dormancy release. Scientia Agricultura Sinica, 38 (2): 376-382.
Szakiel, A., Paczkowski, C. and Henry, M. 2011. Influence of environmental abiotic factors on the content of saponins in plants. Phytochemistry Reviews, 10: 471-491.
Taiz, L. and Zeiger, E. 2012. Plant physiology. Fifth ed., Sinauer Associates Inc., Publishers. Sunderland, Massachusetts U.S.A. 623p.
Takayama, T., Toyomasu, T., Yamane, H., Murofushi, N. and Yajima, H. 1993. Identification of gibberellin and abscisic acid in bulbs of Lilium elegans Thunb. and their quantitative changes during cold treatment and the subsequent cultivation. Journal of the Japanese Society for Horticultural Science, 62: 189-196.
Tsukamoto, Y. 1971. Changes of endogenous growth substances in Easter lily as affected by cooling. Acta Horticulturae, 23: 75-81.
Wang, W., Su, X., Tian, Z., Liu, Y., Zhou, Y. and He, M. 2018. Transcriptome profiling provides insights into dormancy release during cold storage of Lilium pumilum. BMC Genomic, 19. https://doi.org/10.1186/s12864-018-4536-x
Wang, Z.X., Yang, S., Du, Y.P. and Jia, G.X. 2013. Changes of morphological and physiological indexes during low temperature storage for the bulbs of Lilium OT hybrid ‘Manissa’. Journal of Fujian Agriculture and Forestry University, 42 (1): 29-34.
Wu, H., Zheng, S., Bi, Y. and Zhu, Y. 2010. Saponins: A possible relation with Fusarium oxysporum f.sp. lilii resistance in lilies (Lilium L.). Acta Horticulturae, 855: 295-298.
Xu, R.Y., Niimi, Y. and Han, D.S. 2006. Changes in endogenous abscisic acid and soluble sugars levels during dormancy release in bulbs of Lilium rubellum. Scientia Horticulturae, 111: 68-72.
Zhang, M. and Jia, G. 2014. The effects of sucrose concentration and light condition on ‘lily’s’ bulblet-in-tube production and inclusion content. Pakistan Journal of Botany, 46(1): 307-315.
Zhang, Y.J., Xie, Z.K., Wang, Y.J. and An, L.P. 2011. Changes in carbohydrate metabolism and bulb growth as induced by low-temperature release of dormancy in lily bulbs. Philippine Agricultural Scientist, 94 (2): 149-154.
