The Effect of Ascorbic Acid Foliar Application on the Ecophysiological Characteristics of Catharanthus roseus L. Under Water Deficit Stress
محورهای موضوعی : مجله گیاهان زینتی
1 - Department of Agriculture, Astara Branch, Islamic Azad University, Astara, Iran
کلید واژه: Drought stress, Ascorbic acid, dry weight, Catalase, Ornamental and medicinal plants,
چکیده مقاله :
Water is one of the most important environmental factors that regulate plant growth and development, and water deficit is considered the most important restricted factor for plant products, in that several chemical materials have been used to reduce the harmful effects of water deficit. One of these compounds is ascorbic acid and it has antioxidant effects in plants. For this purpose, a factorial pot experiment in the form of a completely randomized design with three replications was conducted to investigate the effect of water deficit on the morphological and physiological characteristics of Catharanthus roseus (Cape periwinkle) under ascorbic acid foliar spraying in 2019. The test factors include: Drought stress (I) at 4 levels based on field capacity (FC): 1) Control (no stress) (I1) Irrigation at 100% FC, 2) Mild stress (I2) Irrigation at 75% FC; 3) Medium stress (I3) Irrigation at 50% FC; and 4) Severe stress (I4) Irrigation at 25% FC; foliar application of ascorbic acid was at 4 levels: 0, 25, 50, 100 mM. The seeds of C. roseus were sown in a 50% field soil +30% peat + 20% perlite substrate. The obtained results showed that the concentration of chlorophyll a and carotenoids, leaf soluble sugar, proline amount and catalase enzyme activity increased with increasing severity of water deficit, but the morphological characteristics of root weight and total biomass decreased. Application levels of ascorbic acid led to the improvement of the measured traits, and in most of the traits, the 50 mM level was significantly superior to other levels. According to the obtained results, it is recommended to use ascorbic acid to improve the growth of plants such as C. roseus under drought stress.
آب یکی از مهمترین عوامل محیطی تنظیم کننده رشد و نمو گیاهان است و کمبود آب مهمترین عامل محدودکننده تولیدات گیاهی به‌شمار می‌رود، به طوری که از چندین ماده شیمیایی برای کاهش اثرات مضر کمبود آب استفاده شده است. یکی از این ترکیبات اسید آسکوربیک است و دارای اثرات آنتی اکسیدانی در گیاهان می‌باشد. به همین منظور یک آزمایش گلدانی به صورت فاکتوریل در قالب طرح پایه کاملاَ تصادفی با سه تکرار جهت بررسی اثر کمبود آب بر خصوصیات مورفولوژیک و فیزیولوژیک پریوش تحت محلول‌پاشی اسید آسکوربیک، در سال 1399 انجام شد. فاکتورهای آزمایش شامل: تنش کمبود آب در چهار سطح (1: بدون تنش آبیاری در100 درصد ظرفیت مزرعه؛ 2: تنش ملایم آبیاری در 75 درصد ظرفیت مزرعه؛ 3: تنش متوسط آبیاری در 50 درصد ظرفیت مزرعه و 4: تنش شدید آبیاری در 25 درصد ظرفیت مزرعه) و محلول‌پاشی اسید آسکوربیک در چهار سطح (صفر، ۲۵، ۵۰، 100 میلی‌مولار) بودند. نتایج به‌دست آمده نشان داد که غلظت کلروفیل a و کاروتنوئید، قند محلول برگ، مقدار پرولین و فعالیت آنزیم کاتالاز با افزایش شدت کمبود آب افزایش یافت ولی صفات وزن خشک ریشه و بیومس کل کاهش یافتند. کاربرد اسید آسکوربیک منجر به بهبود صفات اندازه‌گیری شد و سطوح کاربرد اسید آسکوربیک نسبت به عدم کاربرد آن برتری معنی‌دار داشت و سطح 50 میلی‌مولار نسبت به سایر سطوح، تاثیر بیشتری، در اغلب صفات، داشت. با توجه به نتایج بدست آمده، کاربرد اسید آسکوربیک برای بهبود رشد گیاهانی مانند پریوش تحت تنش خشکی قابل توصیه می‌باشد.
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