First Report of Fusarium oxysporum Causing Fusarium Wilt on Thuja plicata in Iran
محورهای موضوعی : مجله گیاهان زینتی
Hadi Rahanandeh
1
(Department of Plant Pathology, Rasht Branch, Islamic Azad University, Rasht, Iran, P. O. Box 4415866865, Iran)
کلید واژه: Guilan, Fusarium, Conifer, Thuja,
چکیده مقاله :
Thuja is one of the ornamental plants belonging to the conifers and Cupressales order. Coniferous trees are always important as creators of urban green spaces in terms of environmental values due to practical reasons and fast growth. In recent years, thuja wilt disease has caused irreparable damage to urban greenery, and its symptoms appear in the form of weakness and drying of the branches, which eventually causes the death of the tree. This research was conducted in order to investigate the causes of root and collar drying of thuja (Thuja plicata) in the west of Guilan province, during 2021-2022. In this study, samples were taken from the western regions of Guilan province. 40 samples were taken from the infected parts of the root and collar. After culturing the infected parts on the culture media, five fungal isolates were isolated in this study, the pathogenicity of this fungus was confirmed on potted thuja seedlings. Out of five isolates, two isolates showed wilting and rotting of the root and drying of the tips of the leaves with the same intensity of pathogenicity. These two isolates were similar in terms of their shape, and only one isolate was subjected to further laboratory work. Different cultures and molecular methods were used to identify this fungus. Based on the identification key and the molecular method, the disease-causing fungus F. oxysporum was identified.
تویا از گیاهان زینتی متعلق به رده مخروطیان و راسته Cupressales هست. درختان سوزنی برگ به دلایل کاربردی و رشد سریع همواره به عنوان ایجاد کننده فضای سبز شهری در جهت ارزش های زیست محیطی حائز اهمیت هستند. بیماری سرخشکیدگی تویا در سال های اخیر خسارت های جبران ناپذیری را به فضای سبز شهری وارد کرده است و علائم آن به صورت ضعف، خشکیدگی شاخه ها ظهور پیدا می کند که در نهایت موجب مرگ درخت می شود. این تحقیق به منظور بررسی علل خشکیدگی ریشه و طوقه تویا (Thuja plicata) در غرب استان گیلان، طی سال‌های 2022-2021 اجرا گردید. در این بررسی نمونه برداریهایی از مناطق غربی استان گیلان صورت گرفت. ازقسمت های آلوده‌ی ریشه و طوقه 40 نمونه گرفته شد. پس از کشت قطعات آلوده روی محیط‌های کشت در این بررسی پنج جدایه قارچی جدا شد، اﺛﺒﺎﺕ ﺑﻴﻤﺎﺭﻱ ﺯﺍﺋﻲ ﺍﻳﻦ ﻗﺎﺭچ ﺭﻭﻱ ﻧﻬﺎﻟﻬﺎﻱ ﻳﻜﺴﺎﻟﻪ گﻠﺪﺍﻧﻲ ﻭﺧﺰﺍﻧﻪ ﺍﻱ تویا ﺑﻪ ﺍﻧﺠﺎﻡ ﺭﺳﻴﺪ. از پنج جدایه دو جدایه پژمردگی و پوسیدگی ریشه و خشک شدن نوک برگها با شدت یکسان بیماریزایی نشان دادند. این دو جدایه از لحاظ شکل پرگنه مشابه بودند، که تنها روی یک جدایه ادامه کارهای آزمایشگاهی انجام شد. از محیط کشت های مختلف و روش مولکولی برای شناسایی این قارچ استفاده شد. بر اساس کلید شناسایی و روش مولکولی قارچ عامل بیماری F. oxysporum شناسایی شد.
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