اثر تغذیه نمک های کلسیمی روغن های گیاهی بر شاخص های آنتی اکسیدانی خون و غلظت هورمون های موثر بر متابولیسم در بره پرواری در معرض تنش گرمایی

محورهای موضوعی : زیست شناسی جانوری

سید احمد سیدین نیا ¹ , علی اصغر صادقی ² , فرخناز معتمدی سده ³ , محمد چمنی ⁴ , پروین شورنگ ⁵

1 - دانشجوی دوره دکتری، گروه علوم دامی، واحد علوم و تحقيقات، دانشگاه آزاد اسلامي، تهران، ايران
2 - دانشیار، گروه علوم دامی، واحد علوم و تحقيقات، دانشگاه آزاد اسلامي، تهران، ايران
3 - دانشیار پژوهشکده کشاورزی هسته ای، پژوهشگاه علوم و فنون هسته ای، کرج، ایران
4 - استاد، گروه علوم دامی، واحد علوم و تحقيقات، دانشگاه آزاد اسلامي، تهران، ايران
5 - دانشیار پژوهشکده کشاورزی هسته ای، پژوهشگاه علوم و فنون هسته ای، کرج، ایران

تاریخ دریافت : 1402/11/22 تاریخ پذیرش : 1403/06/28 تاریخ انتشار : 1403/11/28

کلید واژه: آنزیم های کبدی, بره پرواری, چربی محافظت شده, گلوتاتیون پراکسیداز, مالون دی آلدئید,

چکیده مقاله :

هدف: یکی از راهکارهای غلبه بر تنش گرمایی، استفاده از منابع انرژی است که سبب بهبود ظرفیت آنتی اکسیدانی بدن شود. اثرات نمک های کلسیمی روغن ها بر عملکرد و پارامترهای تولید مثلی مطالعه شده است ولیکن به اثرات این منابع انرژی بر وضعیت آنتی اکسیدانی و غلظت هورمون های خون کمتر پرداخته شده است. بنابراین، هدف از مطالعه حاضر بررسی اثرات نمکهای کلسیمی روغن پالم، روغن ذرت و روغن کتان بر شاخص های آنتی اکسیدانی و غلظت هورمون های موثر بر متابولیسم در خون بره‌های پرواری تحت تنش گرمایی بود. مواد و روش ها: تعداد 18 راس بره پرواری در قالب طرح کاملا تصادفی در سه گروه آزمایشی و شش تکرار تقسیم شدند. سه جیره آزمایشی حاوی 4 درصد نمک کلسیمی سه روغن شامل روغن پالم، روغن ذرت و روغن کتان تنظیم و به بره های پرواری به مدت 40 روز داده شد. در روز آخر آزمایش، نمونه های خون از ورید وداج جمع آوری شد. غلظت مالون دی آلدئید، ظرفیت آنتی اکسیدانی کل، فعالیت آنزیم های آسپارتات آمینو ترانسفراز وآلانین آمینو ترانسفراز وغلظت هورمون های موثر بر متابولیسم اندازه گیری شد. نتایج: غلظت مالون دی آلدئید در پلاسمای خون بره های تغذیه شده با نمک کلسیمی روغن کتان کمتر و ظرفیت آنتی اکسیدانی آن بیشتر از دو روغن دیگر بود (05/0>P). افزودن نمک کلسیمی روغن کتان سبب افزایش (05/0>P) فعالیت آنزیم های آنتی اکسیدانی شد. بره های دریافت کننده نمک کلسیمی روغن ذرت بیشترین فعالیت آنزیم های مذکور را داشتند (05/0>P) و بین دوگروه دریافت کننده نمک های کلسیمی روغن پالم و روغن کتان تفاوت معنی دار مشاهده نشد (05/0P). غلظت کورتیزول در پلاسمای خون بره های دریافت کننده نمک کلسیمی روغن ذرت بیشتر از بره های دریافت کننده روغن کتان بود (05/0>P). بره های دریافت کننده نمک کلسیمی روغن ذرت غلظت تری یدو تیرونین و تترا یدو تیرونین بیشتری نسبت به دو گروه دیگر داشتند. نتیجه گیری: استفاده از نمک های کلسیمی روغن کتان در تغذیه نشخوارکنندگان بر نمک های کلسیمی روغن سویا ارجحیت دارد و سبب بهبود شاخص های آنتی اکسیدانی و بهبود وضعیت کبد می شود. استفاده از نمک کلسیمی روغن پالم نیز بر روغن ذرت ارجحیت دارد و هر چند سبب بهبود فعالیت آنزیم های آنتی اکسیدانی نشد ولی اثرات منفی بر کبد و هورمون های متابولیسمی نشد.

چکیده انگلیسی:

Aim: One of the suitable method to ameliorate the negative effect of heat stress is to use energy sources that improve the antioxidant capacity of the body. The effects of calcium salts of oils on growth parameters and reproductive performance have been studied, but limited information is available about the effects of these energy sources on antioxidant indices and blood hormone concentrations. Therefore, the aim of this study was to evaluate the effects of calcium salts of palm oil, corn oil and linseed oil on antioxidant indices and the level of metabolism related hormones in the blood of fattening lambs under heat stress. Materials and Methods: Eighteen fattening lambs were divided into three experimental groups and six replicates in a completely random design. Three experimental diets containing 4% calcium salt of oils including palm oil, corn oil and linseed oil were prepared and fed to fattening lambs for 40 days. On the last day of the experiment, blood samples were collected from the jugular vein. The concentration of malondialdehyde, total antioxidant capacity, activity of aspartate aminotransferase and alanine aminotransferase enzymes and the level of metabolism related hormones were measured. Results: The level of malondialdehyde in the plasma of lambs fed with calcium salt of linseed oil was lower and its antioxidant capacity was higher than the other groups (P0.05). The highest blood glucose concentration was observed in the group receiving calcium salt of corn oil (P<0.05). Cortisol concentration in blood plasma of lambs receiving calcium salt of corn oil was higher than lambs receiving linseed oil (P<0.05). The lambs receiving the calcium salt of corn oil had higher concentrations of triiodothyronine and tetraiodothyronine than the other two groups. Conclusion: The application of calcium salts of linseed oil in ruminants feeding is preferable to calcium salts of corn oil, as it improves the antioxidant indices and improves the liver health. The use of calcium salt of palm oil is also preferable to corn oil, and although palm oil did not improve the activity of antioxidant enzymes, it did not have negative effects on the liver and metabolic hormones.

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