آنالیز درون رایانه ای پروتین pMGA1.2 در سویه های واکسن و بیماریزای مایکوپلاسما گالی سپتیکوم

نالیز Insilico برای پروتئین pMGA1.2 در سویه های مایکوپلاسما گالی سپتیکوم

محورهای موضوعی : باکتری شناسی

فرزانه پورکریمی فتیده ¹ (گروه میکروبیولوژی، واحد جهرم، دانشگاه آزاد اسلامی، جهرم، ایران.)
مجید اسمعیلی زاد ² (بخش تحقیق و توسعه، موسسه تحقیقات واکسن و سرم سازی رازی)
محمد کارگر ³ (گروه میکروبیولوژی، دانشگاه آزاد اسلامی، واحد جهرم،)
مجید تبیانیان ⁴ (بخش ایمونولوژی،موسسه تحقیقات و اکسن و سرم سازی رازی)
فرشید کفیل زاده ⁵ (دانشگاه آزاد اسلامی، واحد جهرم، گروه میکروبیولوژی)

تاریخ دریافت : 1402/02/08 تاریخ پذیرش : 1402/04/20 تاریخ انتشار : 1402/07/10

کلید واژه: In silico, مایکوپلاسما گالی سپتیکوم, pMGA1.2 ,

چکیده مقاله :

زمینه و هدف: مایکوپلاسما گالی سپتیکوم، عامل بیماری مزمن تنفسی در جوجه های ماکیان، از نظر اقتصادی مهم ترین گونه مایکوپلاسما است که خسارات اقتصادی فراوان در سراسر جهان ایجاد می کند. فراوان ترین پروتئین های غشایی در مایکوپلاسما گالی سپتیکوم pMGA ، لیپوپروتئین هایی با حدود 67 کیلو دالتون می باشد. ژن‌های خانواده pMGA پتانسیل فوق‌العاده‌ای برای ایجاد تنوع در ساختار آنتی ژنی سطح سلول‌های مایکوپلاسما گالی‌سپتیکوم دارند. هدف از این مطالعه مقایسه الگوهای پروتئین pMGA بین سویه ها و میزبان های مختلف مایکوپلاسما گالی سپتیکوم بود. مواد و روش‌ها: ژنوم‌های کامل مایکوپلاسما گالیسپتیکوم در GenBank تا ژانویه 2020 بررسی و توالی‌های pMGA1.2 شناسایی، گروه‌بندی و کدگذاری شدند. پروتئین pMGA1.2 با طول 650 اسید آمینه بین دو میزبان مختلف (مرغ و فنچ خانگی) توسط نرم افزار بیوانفورماتیک در ژنوم های کامل مایکوپلاسما گالی سپتیکوم مورد بررسی قرار گرفت. یافته‌ها: ژن pMGA1.2 در سویه‌های مختلف مایکوپلاسما گالی‌سپتیکوم پنج گروه اصلی با بیش از 10 درصد واگرایی نشان داد. بر اساس تراز چند توالی، یک الگوی خاص در جدایه فنچ خانگی شناسایی شد. جالب توجه است که دو موتیف خاص 480DNQNVSNQ487 و SNVSSPSY647 639 درژن pMGA1.2 ازسویه TS-11 یافت شد که می تواند به عنوان نشانگر برای شناسایی و تمایز این سویه واکسن از مایکوپلاسما گالی سپتیکوم بیماری زا استفاده شود. نتیجه گیری: در این مطالعه نشان داده شد که پروتئین pMGA1.2 دارای نواحی آنتی ژنی اپی توپ سلول- B است که در تمام ایزوله ها حفظ شده است و می تواند در طراحی تست سرولوژیکی برای تشخیص آنتی بادی علیه مایکوپلاسما گالی سپتیکوم قابل استفاده باشد.

چکیده انگلیسی:

Background & Objectives: Mycoplasma gallisepticum, the pathogen responsible for chronic respiratory disease in chickens, is the most economically important species of Mycoplasma that causing tremendous economic losses worldwide. The most abundant membrane proteins in M. gallisepticum are pMGA, lipoproteins of about 67 kDa. The pMGA family genes have an extraordinary potential for diversifying antigenic structure on the surface of Mycoplasma gallisepticum cells. The aim of this study was to compare the pMGA protein patterns between different strains and hosts of Mycoplasma gallisepticum. Material & Methods: All Mycoplasma gallisepticum full genomes available in GenBank till January 2020 were considered and pMGA1.2 sequences were identified, grouped and coded. pMGA1.2 protein with a chain of 650 amino acids between two different hosts (poultry and house finch) was studied by bioinformatics software in all Mycoplasma gallisepticum full genomes. Results: pMGA1.2 gene among different strains of Mycoplasma gallisepticum showed five major groups with more than 10 percent divergence. Based on multiple sequence alignment, a specific pattern was identified in house finch isolates. Interestingly, two specific motifs 480DNQNVSNQ487 and 639SSNVSSPSY647 were found in the pMGA1.2 of TS-11 strain, which can probably be used as markers to identify and differentiate this vaccine strain from pathogenic Mycoplasma gallisepticum. Conclusion: This study showed that pMGA1.2 protein have some B-cell epitope antigenic regions that are conserved among all isolates and might be applicable to design serological test for detection antibody against Mycoplasma gallisepticum.

منابع و مأخذ:

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