Application of Response Surface Methodology for Xanthan Gum and Biomass Production Using Xan-thomonas campestris

محورهای موضوعی :

Hadi Hashemi Gahruie ¹ (Department of Food Science and Technology, College of Agriculture, Shiraz University, Shir)
Marzieh Moosavi-Nasab ² (Department of Food Science and Technology, College of Agriculture, Shiraz University, Shiraz, Iran Seafood Processing Research group, College of Agriculture, Shiraz University, Shiraz, Iran)
Esmaeil Ziaee ³ (Department of Food Science and Technology, College of Agriculture, Shiraz University, Shiraz, Iran)

تاریخ دریافت : 1394/04/13 تاریخ پذیرش : 1397/08/07 تاریخ انتشار : 1394/06/23

کلید واژه: response surface methodology, Xanthomonas cam-pestris, Xanthan gum, Carbon sources Nitro-gen sources,

چکیده مقاله :

   Xanthan gum is an extracellular polysaccharide produced by various Xanthomonas species such as X. campestris. The objective of present study was to investigate the influence of different carbon and nitrogen sources on xanthan gum production by X. campestris. Using an experimental Response Surface Methodology (RSM) complemented with a Central Composite Design (CCD), the impact of peptone, lactose, glucose and ammonium nitrate in medium were estimated for their individual and interactive effects on biomass and xanthan gum production. The optimal concentrations of peptone, lactose, glucose and ammonium nitrate for xanthan gum yield and biomass production was determined as 9.25 g/l, 53.37 mmol, 29.31 mmol and 4.58 g/l for xanthan gum yield and 6.77 g/l, 52.65 mmol, 38.12 mmol and 3.54 g/l for biomass production. Under the optimum experimental conditions, the xanthan gum yield reached to its maximum value (8.42 g/l). The results provide the support data for xanthan gum production on a large scale. 

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