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  • Study of Potato (Solanum tuberosum L. Agria Cultivar) Microtuberization and Physiological Properties in Salinity Stress via Tissue Culture Technique
کد مقاله : JCHR-2302-1694 (R1) بازدید : 96 PDF XML

نوع مقاله: پژوهشی

Study of Potato (Solanum tuberosum L. Agria Cultivar) Microtuberization and Physiological Properties in Salinity Stress via Tissue Culture Technique

Nosrat Musavi 1 (Department of Plant Physiology, Damghan Branch, Islamic Azad University, Damghan, Iran) Mostafa Ebadi 2 (Department of Plant Physiology, Damghan Branch, Islamic Azad University, Damghan, Iran)
Mehdi Khorshidi 3 (Assistant Professor, Biology School, Damghan University, Damghan, Iran) Nahid Masoudian 4 (Department of Plant Physiology, Damghan Branch, Islamic Azad University, Damghan, Iran) Hossein Hokmabadi 5 (Associate Professor, Agricultural Education and Extension Institute, AREEO, Tehran, Iran)

تاریخ ارسال : 1401/12/07 تاریخ تایید : 1402/03/29

کلید واژه: salinity stress, tissue culture, Physiological properties, Potato,

چکیده مقاله :

One of the most important crop plants is potato. Plant tissue culture is a very important technology in plant cultivation. In this investigation, the biochemical characterizes are investigated of potatoes cultivated by the tissue culture method. The lateral and apical buds were separated from the stem. They were sterilized and cultured in Murashige and Skoog culture medium. The concentrations of salt (0, 25, 50, 75 and 100 mM) have been used for salinity. After the growth of buds, the leaves were sampled and the chlorophylls, proline, sugars, protein, and antioxidant enzymes activity such as catalase, superoxide dismutase, guaiacol peroxidase, glutathione reductase and ascorbate peroxidase were evaluated. The results indicate that salt decreased protein and chlorophylls content. Sugars content and fresh weight have also been reduced with salinity, but the content of proline, malondialdehyde, and hydrogen peroxide were increased under salinity stress. Phenolic compounds, anthocyanin content, and antioxidant enzymes activity increased up to 50 mM salinity, but they decreased above this concentration. According to these results, it can be suggested that Agria cultivar potatoes are not recommended in salinity higher than 50 mM.

چکیده انگلیسی :

One of the most important crop plants is potato. Plant tissue culture is a very important technology in plant cultivation. In this investigation, the biochemical characterizes are investigated of potatoes cultivated by the tissue culture method. The lateral and apical buds were separated from the stem. They were sterilized and cultured in Murashige and Skoog culture medium. The concentrations of salt (0, 25, 50, 75 and 100 mM) have been used for salinity. After the growth of buds, the leaves were sampled and the chlorophylls, proline, sugars, protein, and antioxidant enzymes activity such as catalase, superoxide dismutase, guaiacol peroxidase, glutathione reductase and ascorbate peroxidase were evaluated. The results indicate that salt decreased protein and chlorophylls content. Sugars content and fresh weight have also been reduced with salinity, but the content of proline, malondialdehyde, and hydrogen peroxide were increased under salinity stress. Phenolic compounds, anthocyanin content, and antioxidant enzymes activity increased up to 50 mM salinity, but they decreased above this concentration. According to these results, it can be suggested that Agria cultivar potatoes are not recommended in salinity higher than 50 mM.

منابع و مأخذ:


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