The Developmental Competence of the Bovine Embryos Processed in IVC-Stroebech Medium vs. Mcr2aa Medium
محورهای موضوعی : Large Ruminant ReproductionM. Zandi 1 , A. Ghaedrahmati 2 , L. Stroebech 3
1 - Department of Agriculture, Iranian Research Organization for Science and Technology (IROST), Tehran, Iran
2 - Department of Animal Science, Agricultural Science and Natural Resources University of Khuzestan, Mollasani, Khuzestan, Iran
3 - Department of Veterinary and Animal Science, University of Copenhagen, Denmark
کلید واژه: blastocysts, define media, in vitro culture, in vitro fertilization, undefined media,
چکیده مقاله :
Fetal bovine serum (FBS) is preferred for its ability to provide a variety of molecules that can support the growth of embryos. However, the use of serum can lead to the development of large offspring syndrome, which is a significant problem. This study aimed to examine bovine embryo development derived from in vitro fertilized oocytes in two culture media: IVC-Stroebech medium, a chemically defined medium; and the modified medium of Charles Rosenkrans with amino acids (mCR2aa), an undefined medium containing serum. The process of in vitro maturation was carried out at 38.5 ˚C in IVC-Stroebech medium. The process of in vitro fertilization was carried out for 18 hours using Bracket and Oliphant's (BO) medium. As to the experiment, cumulus-free presumptive zygotes were randomly assigned to two different culture medium treatments. The first treatment was a two-step culture system using mCR2aa medium. The first step in-volved using mCR2aa medium without FBS for the first 48 hours of culture. After that, for the remaining days of culture, the second-step mCR2aa, which is composed of 10% FBS, replaced the medium. The em-bryos were incubated at 38.5 ˚C under 5% CO2 5% O2 and 90% N2 for 10 days. Statistical analysis re-vealed a significantly higher hatched blastocyst rate in the IVC-Stroebech medium in comparison with the mCR2aa medium. There was not any significant difference (P>0.05) in the formation rate of morulas and blastocysts between the IVC-Stroebech medium and the mCR2aa medium. Findings recommended the use of IVC-Stroebech medium for bovine in vitro embryo culture.
Fetal bovine serum (FBS) is preferred for its ability to provide a variety of molecules that can support the growth of embryos. However, the use of serum can lead to the development of large offspring syndrome, which is a significant problem. This study aimed to examine bovine embryo development derived from in vitro fertilized oocytes in two culture media: IVC-Stroebech medium, a chemically defined medium; and the modified medium of Charles Rosenkrans with amino acids (mCR2aa), an undefined medium containing serum. The process of in vitro maturation was carried out at 38.5 ˚C in IVC-Stroebech medium. The process of in vitro fertilization was carried out for 18 hours using Bracket and Oliphant's (BO) medium. As to the experiment, cumulus-free presumptive zygotes were randomly assigned to two different culture medium treatments. The first treatment was a two-step culture system using mCR2aa medium. The first step in-volved using mCR2aa medium without FBS for the first 48 hours of culture. After that, for the remaining days of culture, the second-step mCR2aa, which is composed of 10% FBS, replaced the medium. The em-bryos were incubated at 38.5 ˚C under 5% CO2 5% O2 and 90% N2 for 10 days. Statistical analysis re-vealed a significantly higher hatched blastocyst rate in the IVC-Stroebech medium in comparison with the mCR2aa medium. There was not any significant difference (P>0.05) in the formation rate of morulas and blastocysts between the IVC-Stroebech medium and the mCR2aa medium. Findings recommended the use of IVC-Stroebech medium for bovine in vitro embryo culture.
Abe H., Yamashita S., Satoh T. and Hoshi H. (2002). Accumulation of cytoplasmic lipid droplets in bovine embryos and cryotolerance of embryos developed in different culture systems using serum‐free or serum-containing media. Mol. Reprod. Dev. 61(1), 57-66.
Dunning K.R., Cashman K., Russell D.L., Thompson J.G., Norman R.J. and Robker R.L. (2010). Beta-oxidation is essential for mouse oocyte developmental competence and early embryo development. Biol. Reprod. 83(6), 909-918.
Ferré L.B., Kjelland M.E., Strøbech L.B., Hyttel P., Mermillod P. and Ross P.J. (2020). Recent advances in bovine in vitro embryo production: reproductive biotechnology history and methods. Animal. 14(5), 991-1004.
Garcia S.M., Marinho L.S.R., Lunardelli P.A., Seneda M.M. and Meirelles F.V. (2015). Developmental block and programmed cell death in Bos indicus embryos: Effects of protein supplementation source and developmental kinetics. PLoS One. 10(3), e0119463.
Goel P., Goel A.K., Bhatia A.K. and Kharche S.D. (2016). Comparative study on effect of different embryo culture media on in vitro blastocyst production in goats. Indian J. Anim. Sci. 86(10), 1115-1120.
Gómez E. and Diez C. (2000). Effects of glucose and protein sources on bovine embryo development in vitro. Anim. Reprod. Sci. 58(1), 23-37.
Gómez E., Rodríguez A., Muñoz M., Caamaño J.N., Hidalgo C.O., Morán E. and Díez C. (2008). Serum free embryo culture medium improves in vitro survival of bovine blastocysts to vitrification. Theriogenology. 69(8), 1013-1021.
Gstraunthaler G. (2003). Alternatives to the use of fetal bovine serum: serum-free cell culture. ALTEX-Altern. Anim. Exp. 20(4), 275-281.
Hajian M., Jafarpour F., Aghamiri S.M., Varnosfaderani S.R., Rahimi M. and Nasr-Esfahani M.H. (2020). The impact of two embryo culture media, SOF and commercial BO, on pre-and post-implantation development of cloned sannen goat embryos. Int. J. Fertil. Steril. 16(1), 23-29.
Jafarnejad A., Zandi M., Aminafshar M., Sanjabi M.R. and Emamjomeh Kashan N. (2018). Evaluating bovine sperm transfection using a high-performance polymer reagent and as-sessing the fertilizing capacity of transfected spermatozoa us-ing an in vitro fertilization technique. Arch. Anim. Breed. 61, 351-358.
Karimian M., Zandi M., Sanjabi M.R., Masoumian M. and Ofoghi H. (2018). Effects of grape seed extract, quercetin and vitamin C on ovine oocyte maturation and subsequent embryonic de-velopment. Cell. Mol. Biol. 64(4), 98-102.
Kim S.H. and Yoon J.T. (2019). Effect of serum-containing and serum-free culture medium-mediated activation of matrix metalloproteinases on embryonic developmental competence. Czech J. Anim. Sci. 64(12), 473-482.
Mesalam A., Lee K.L., Khan I., Chowdhury M.M.R., Zhang S., Song S.H. and Kong I.K. (2019). A combination of bovine serum albumin with insulin–transferrin–sodium selenite and/or epidermal growth factor as alternatives to fetal bovine serum in culture medium improves bovine embryo quality and trophoblast invasion by induction of matrix metalloproteinases. Reprod. Fertil. Dev. 31(2), 333-346.
Murillo A., Muñoz M., Martín-González D., Carrocera S., Martínez-Nistal A. and Gómez E. (2017). Low serum concentration in bovine embryo culture enhances early blastocyst rates on day-6 with quality traits in the expanded blastocyst stage similar to BSA-cultured embryos. Reprod. Biol. 17(2), 162-171.
Muzaffar M., Selokar N.L., Singh K.P., Zandi M., Singh M.K., Shah R.A. Chauhan M.S. Singla S.K. Palta P. and Manik R. (2012). Equivalency of buffalo (Bubalus bubalis) embryonic stem cells derived from fertilized, parthenogenetic and handmade cloned embryos. Cell. Reprogram. 14(3), 267-279.
Naitana S. and Ledda S. (2020). Reproductive technologies in sheep. Pp. 31-54 in Reproductive Technologies in Animals. G.A. Presicce, Ed., Academic Press, Elsevier, Amsterdam, the Netherlands.
Paschoal D.M., Sudano M.J., Guastali M.D., Maziero R.R.D., Crocomo L.F., Magalhães L.C.O. and da Cruz Landim-Alvarenga F. (2014). Forskolin effect on the cryosurvival of in vitro-produced bovine embryos in the presence or absence of fetal calf serum. Zygote. 22(2), 146-157.
Poniedziałek-Kempny K., Gajda B., Rajska I., Gajda L. and Smorąg Z. (2019). Piglets produced by transfer of embryos obtained by in vitro fertilization of oocytes matured in vitro with thymosin: A case report. Med. Weter. 76(3), 181-185.
Pryor J.H., Hasler J.F., Strøbech L., Avery B., Hashem N., Menges S. and Looney C.R. (2016). Improved bovine embryo production using novel in vitro culture systems. Reprod. Fertil. Dev. 28(2), 172-172.
Rauch C., Feifel E., Amann E.M., Spötl H.P., Schennach H., Pfaller W. and Gstraunthaler G. (2011). Alternatives to the use of fetal bovine serum: human platelet lysates as a serum substitute in cell culture media. ALTEX-Altern. Anim. Exp. 28(4), 305-316.
Rizos D., Gutierrez-Adan A., Perez-Garnelo S., De La Fuente J., Boland M.P. and Lonergan P. (2003). Bovine embryo culture in the presence or absence of serum: implications for blastocyst development, cryotolerance, and messenger RNA expression. Biol. Reprod. 68(1), 236-243.
Romão R., Marques C., Bettencourt E. and Pereira R. (2016). Cryopreservation of Sheep Produced Embryos–Current and Future Perspectives. University of Évora, Portugal.
Shahzad Q., Xu H.Y., Pu L., Waqas M., Wadood A.A., Xie L. and Lu Y. (2020). Developmental potential of buffalo embryos cultured in serum free culture system. Theriogenology. 149, 38-45.
SPSS Inc. (2011). Statistical Package for Social Sciences Study. SPSS for Windows, Version 20. Chicago SPSS Inc., USA.
Sudano M.J., Paschoal D.M., da Silva Rascado T., Magalhães L.C.O., Crocomo L.F., de Lima-Neto J.F. and da Cruz Landim-Alvarenga F. (2011). Lipid content and apoptosis of in vitro-produced bovine embryos as determinants of susceptibility to vitrification. Theriogenology. 75(7), 1211-1220.
Taub M. (1990). The use of defined media in cell and tissue culture. Toxicol. In Vitro. 4(3), 213-225.
Wan P.C., Hao Z.D., Zhou P., Wu Y., Yang L., Cui M.S. and Zeng S.M. (2009). Effects of SOF and CR1 media on developmental competence and cell apoptosis of ovine in vitro fertilization embryos. Anim. Reprod. Sci. 114(1), 279-288.
Zhu J., Moawad A.R., Wang C.Y., Li H.F., Ren J.Y. and Dai Y.F. (2018). Advances in in vitro production of sheep embryos. Int. J. Vet. Sci. Med. 6, 15-26.
