ارزیابی فیتوشیمیایی، آنتی¬اکسیدانی و ضدآلزایمری نانوذرات نقره سنتز شده با عصاره آبی گیاه خارشتر (Alhagi persarum)
محورهای موضوعی : بیوشیمی
نیلوفر واحدپوری
1
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امیر آراسته
2
1 - گروه زیست شناسی، واحد رشت، دانشگاه آزاد اسلامی، رشت، ایران
2 - عضو هیئت عملی رشت، دانشگاه آزاد اسلامی واحد رشت، دانشکده ی علوم پایه، گروه میکروبیولوژی
کلید واژه: Alhagi persarum, آنتی اکسیدان, استیل کولین استراز, بیماری آلزایمر, نانوذره,
چکیده مقاله :
گیاه دارویی خارشتر (Alhagi persarum) از گیاهان مقاوم مناطق خشک است که جایگاه ویژهای در طب سنتی ایرانی دارد. هدف از این پژوهش بررسی اثرات آنتیاکسیدانی، فیتوشیمیایی و مهاری نانوذرات نقره سنتز شده با عصاره آبی خارشتر بر تولید نانورشته های آمیلوئیدی بوده است. ترکیبات موجود در عصاره با گاز کروماتوگرافی جرمی و میزان مهار رادیکالهای آزاد با آزمون DPPH و مهار آنزیم استیلکولین استراز با طیفسنجی مرئی بررسی گردید. مهار تولید رشتههای آمیلوئیدی با طیفسنجی فلورسانس بررسی و با میکروسکوپ الکترونی گزاره تائید گردید. تولید نانوذرات نقره با طیفسنجی مرئی و مادون قرمز تائید شد و ساختار آن با روش پراکندگی نور دینامیکی (DLS)، پتانسیل زتا، میکروسکوپ الکترونی روبشی (SEM) و طیفسنجی پراش پرتو ایکس (XRD) بررسی شد. بیشترین ترکیبات موجود در عصاره تیوفن، اکتادکانوئیک اسید و اینوزیتول بود. غلظت نانوذرات با فعالیت آنتیاکسیدانی رابطه مستقیم داشت و با اثرات مهاری بر آنزیم استیلکولین استراز و مهار رشتههای آمیلوئیدی رابطه عکس نشان داد. عصاره گیاه A. persarum، میتواند بهعنوان یک ترکیب مکمل غذایی مناسب برای کاهش عوارض بالینی در مبتلایان به آلزایمر مورد استفاده قرار گیرد.
The medicinal plant Alhagi persarum is known for its resilience in arid regions and holds an important place in traditional Iranian medicine. This study aimed to investigate the antioxidant properties, phytochemical content, and inhibitory effects of silver nanoparticles (AgNPs), which were synthesized using an aqueous extract of A. persarum, on the production of amyloid nanofibers. The compounds present in the extract were analyzed using gas chromatography-mass spectrometry (GC-MS), the inhibition rate of free radicals was measured using the DPPH assay, and the inhibition rate of the acetylcholinesterase enzyme was assessed using spectrometric methods. The inhibition of amyloid fibril production was examined using fluorescence spectroscopy and confirmed through scanning electron microscopy. The production of AgNPs was verified by both visible and infrared spectroscopy, and their structure was analyzed using dynamic light scattering (DLS), zeta potential measurements, scanning electron microscopy (SEM), and X-ray diffraction spectroscopy (XRD). The extract contained a variety of compounds, with thiophene, octadecanoic acid, and inositol being the most abundant. There was a direct relationship between the concentration of nanoparticles and antioxidant activity, whereas an inverse relationship was observed between nanoparticle concentration and the inhibitory effects on the acetylcholinesterase enzyme as well as the inhibition of amyloid fibril formation. The A. persarum plant extract shows promise as a dietary supplement to help mitigate clinical complications in patients with Alzheimer's disease.
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