برداشت ریزجلبک Nannochloropsis oculata به روش لخته¬سازی الکتروشیمیایی توسط الکترودهای آهن، آلومینیوم و گرافیت
محورهای موضوعی : نشریه فن آوریهای نوین در توسعه آبزی پروری
1 - گروه شیلات، دانشگاه آزاد اسلامی، واحد علوم و تحقیقات، تهران، ایران.
کلید واژه: Nannochloropsis oculata, برداشت جلبک, الکتروشیمی, انعقاد الکتریکی ,
چکیده مقاله :
برداشت ريزجلبک یک فرایند دو مرحله‌ای است که شامل جداسازی فاز مایع رویی از زی¬توده یا فاز جامد است که بعد از تولید انبوه آغاز می¬شود. هدف از این تحقیق جمع¬آوری ریزجلبک Nannochloropsis oculata به روش لخته¬سازي الکتروشیمیایی توسط الکترودهای مختلف آهن، آلومینیوم و گرافیت و اثر آن‌ها بر بازده برداشت، تعداد سلول‌های ریزجلبک بعد از برداشت و نرخ لخته شدن ریزجلبک بود، که طی مدت 20 دقیقه ارزیابی شد. بیشترین مقدار بازده برداشت به طور همزمان در تیمار شاهد (استفاده از سانتریفیوژ) و الکترود آلومینیوم اندازه‌گیری شد (05/0>P). نتایج این مطالعه نشان داد که برداشت الکتروشیمایی ریز جلبک N. oculata توسط الکترودهای فلزی (آهن و آلومینیوم) و غیرفلزی (گرافیت) سبب تغییرات معنی‌داری در تعداد سلول‌ها بعد از برداشت، مقدار خاکستر و غلظت لخته¬سازي جلبک داشت و تیمار الکترود آلومینیوم بالاترین بازده برداشت جلبک را نشان داد (48/1 ± 47/96 درصد) و کمترین بازده در الکترود گرافیت (c33/1 ± 92/74 درصد) مشاهده شد (05/0>P). همچنين نرخ لخته شدن ريز جلبک نانوکلرپسیس اوکولاتا در تيمار آلومينيوم سريع¬تر اتفاق افتاد (05/0>P). بنابراين با توجه به نتایج به دست آمده در این تحقیق، انعقاد الکتریکی (Electrocoagulation) با الکترود آلومينيوم روشی موثر برای لخته‌سازي و برداشت سلول‌های میکروجلبک نانوکلرپسیس اوکولاتا بوده، از این رو می¬تواند به عنوان یک روش مناسب و جایگزینی سودآور در بازیابی زی¬توده و تولید ميکروجلبک نانوکلرپسیس تغلیظ شده برای استفاده در صنایع مختلف از جمله بیودیزل پیشنهاد نمود.
Microalgae harvesting is a two-step process that included the separation of supernatant liquid phase from biomass or solid phase that starts after mass production. The purpose of this research is to collect Nannochloropsis oculata microalgae by electrocoagulation method with different electrodes (iron, aluminum and graphite), and their effect on harvesting efficiency, the number of microalgae cells after harvesting and the coagulation rate of microalgae, which was evaluated during 20 minutes. The highest harvesting efficiency was measured in the control and aluminum electrode treatments (P<0.05). The results of this study showed that the electrochemical harvesting of microalgae N. oculata by metallic (iron and aluminum) and non-metallic (graphite) electrodes caused significant changes in the number of cells after harvesting, the amount of ash and the concentration of algae. Aluminum electrode treatment showed the highest algae harvesting efficiency (96.47±0.48%) and the lowest harvesting efficiency was observed in graphite electrode (74.92±1.33%) (P<0.05). Also, the coagulation rate of microalgae Nansoclepis oculata was faster in aluminum treatment (P<0.05). According to the results obtained in this research, electrocoagulation with aluminum electrode is an effective method for coagulation and harvesting of N. oculata microalgae cells. Therefore, it can be used as a suitable method and a profitable alternative in the recovery of biomass and the production of concentrated N. oculata microalgae for various industries such as biodiesel.
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