The Effect of Aerobic and Resistance Training on Gene Expression and Protein Levels of ANP in Male Wistar Rats

محورهای موضوعی : Report of Health Care

Majid Azadmanesh ¹ (Department of Physical Education, Neyshabur Branch, Islamic Azad University, Neyshabur, Iran)
Amir Rashidlamir ² (Department of Sport Physiology, Ferdowsi University of Mashhad, Mashhad, Iran)
Seyed Mahmoud Hejazi ³ (Department of Physical Education, Mashhad Branch, Islamic Azad University, Mashhad, Iran)

تاریخ دریافت : 1397/06/19 تاریخ پذیرش : 1397/06/21 تاریخ انتشار : 1397/07/09

کلید واژه: Training, ANP, Heart,

چکیده مقاله :

Introduction: Hypertension is one of the common diseases in modern societies. ANP hormone is one of the factors regulating blood pressure. Apart from conventional clinical treatments, recent attention has been paid to the impact of exercise on ANP. But there is still debate around the type of exercise that is being used. Therefore, the aim of this study was to investigate the effect of aerobic and resistance training on gene expression and protein levels of ANP in male Wistar rats.
Methods: In this experimental study, 15 male Wistar rats were divided into 3 groups of 5 subjects including (1) control, (2) aerobic training, and (3) resistance training. Aerobic training included running on treadmill at 20 m/min (5 sessions, each session 60 minutes per week), while resistance training included climbing a ladder of one meter with 85-degree slope. For analyzing the findings, one-way ANOVA with Tukey's post hoc test were run using SPSS version 19 (p<0.05). Results: aerobic training had a significant effect on the increased levels of the ANP gene expression (p = 0.001) and ANP tissue concentration (p = 0.001) in the heart tissue of rats. Resistance training had a significant effect on the increased levels of ANP gene expression (p = 0.001) and ANP tissue concentration (p = 0.001) in the heart tissue of rats. Also, aerobic training had a greater effect on the ANP gene expression and ANP tissue concentration in the heart tissue of rats compared to resistance training (p = 0.001). Conclusion: It seems that aerobic and resistance training with a significant increase in ANP gene expression and ANP concentration, elevate heart health, however, the effect of aerobic training in this field was more than resistance training.

منابع و مأخذ:

1. Gorgui J, Gorshkov M, Khan N, Daskalopoulou SS. Hypertension as a risk factor for ischemic stroke in women. CJC. 2014; 30 (7): 774- 782.


2. Johansson BB. Hypertension mechanisms causing stroke. Clin Exp Pharmacol Physiol. 1999; 26 (7): 563- 565.


3. Cardoso CJ, Gomides RS, Queiroz AC, Pinto LG, da Silveira Lobo F, Tinucci T, et al. Acute and chronic effects of aerobic and resistance exercise on ambulatory blood pressure. Clinics. 2010; 65 (3): 317- 325.


4. Cornelissen VA, Buys R, Smart NA. Endurance exercise beneficially affects ambulatory blood pressure: a systematic review and meta-analysis. Am J Hypertens. 2013; 31 (4): 639- 648.


5. Moraes MR, Bacurau RF, Casarini DE, Jara ZP, Ronchi FA, Almeida SS, et al. Chronic conventional resistance exercise reduces blood pressure in stage 1 hypertensive men. J Strength Cond Res. 2012; 26 (4): 1122- 1129.


6. Fagard RH. Exercise is good for your blood pressure: effects of endurance training and resistance training. Clin Exp Pharmacol Physiol. 2006; 33 (9): 853- 856.


7. de Almeida JC, Alves CL, de Abreu LC, Sato MA, Fonseca FL, de Mello Monteiro CB, et al. Involvement of the atrial natriuretic peptide in cardiovascular pathophysiology and its relationship with exercise. Int Arch Med. 2012; 5 (1): 4.


8. Maria Sarullo F, Gristina T, Brusca I, Milia S, Raimondi R, Sajeva M, et al. Effect of physical training on exercise capacity, gas exchange and N-terminal pro-brain natriuretic peptide levels in patients with chronic heart failure. Eur J Cardiovasc Prev Rehabil. 2006; 13 (5): 812- 817.


9. Birkenfeld AL, Boschmann M, Moro C, Adams F, Heusser K, Tank J, et al. Beta-adrenergic and atrial natriuretic peptide interactions on human cardiovascular and metabolic regulation. J Clin Endocrinol Metab. 2006; 91 (12): 5069- 5075.


10. Moro C, Pasarica M, Elkind-Hirsch K, Redman LM. Aerobic exercise training improves atrial natriuretic peptide and catecholamine-mediated lipolysis in obese women with polycystic ovary syndrome. J Clin Endocrinol Metab. 2009; 94 (7): 2579- 2586.


11. Ohba H, Takada H, Musha H, Nagashima J, Mori N, Awaya T, et al. Effects of prolonged strenuous exercise on plasma levels of atrial natriuretic peptide and brain natriuretic peptide in healthy men. Am Heart J. 2001; 141 (5): 751- 758.


12. Raha D, Tortorella C, Neri G, Prasad A, Raza B, Raskar R, et al. Atrial natriuretic peptide enhances cortisol secretion from guinea-pig adrenal gland: evidence for an indirect paracrine mechanism probably involving the local release of medullary catecholamines. Int J Mol Med. 2006; 17 (4): 633- 636.


13. Braith RW, Welsch MA, Feigenbaum MS, Kluess HA, Pepine CJ. Neuroendocrine activation in heart failure is modifiedby endurance exercise training. J Am Coll Cardiol. 1999; 34 (4): 1170- 1175.


14. Niessner A, Ziegler S, Slany J, Billensteiner E, Woloszczuk W, Geyer G. Increases in plasma levels of atrial and brain natriuretic peptides after running a marathon: are their effects partly counterbalanced by adrenocortical steroids?. Eur J Endocrinol. 2003; 149 (6): 555- 559.


15. Freund BJ, Wade CE, Claybaugh JR. Effects of exercise on atrial natriuretic factor. Release mechanisms and implications for fluid homeostasis. J Sports Med. 1988; 6 (6): 364- 377.


16. Geny B, Saini J, Mettauer B, Lampert E, Piquard F, Follenius M, et al. Effect of short-term endurance training on exercise capacity, haemodynamics and atrial natriuretic peptide secretionin heart transplant recipients. Eur J Appl Physiol. 1996; 73 (3- 4): 259- 266.


17. Miller TD, Rogers PJ, Bauer BA, Burnett JC, Bailey KA, Bove AA. What stimulates atrial natriuretic factor release during exercise?. J Lab Cli Med. 1990; 116 (4): 487- 491.


18. Pan SS. Alterations of atrial natriuretic peptide in cardiomyocytes and plasma of rats after different intensity exercise. Scand J Med Sci Sports. 2008; 18 (3): 345- 353.


19. Passino C, Severino S, Poletti R, Piepoli MF, Mammini C, Clerico A, et al. Aerobic training decreases B-type natriuretic peptide expression and adrenergic activation in patients with heart failure. J Am Coll Cardiol. 2006; 47 (9): 1835- 1839.


20. Kraemer WJ, Ratamess NA. Fundamentals of resistance training: progression and exercise prescription. Med Sci Sports Exerc. 2004; 36 (4): 674- 688.


21. Pratley R, Nicklas B, Rubin M, Miller J, Smith A, Smith M, et al. Strength training increases resting metabolic rate and norepinephrine levels in healthy 50- to 65-yr-old men. Eur J Appl Physiol. 1994; 76 (1): 133- 137.


22. Lipari EF, Lipari D, Valentino B. Modifications of atrial natriuretic peptide and vasopressin peptides in the rat hypothalamic supraoptic nucleus during resistance training. Ital J Anat Embryol. 2010; 115 (3): 211- 217.


23. Andersen K, Jonsdottir S, Sigurethsson AF, Sigurethsson SB. The effect of physical training in chronic heart failure. Laeknabladid. 2006; 92 (11): 759- 764.


24. Porsti I, Kahonen M, Wu X, Arvola P, Ruskoaho H. Long-term physical exercise and atrial natriuretic peptide in obese Zucker rats. Basic Clin Pharmacol Toxicol. 2002; 91 (1): 8- 12.


25. Suda K, Hagiwara H, Kotani Y, Kato K, Sasaki M, Izawa T, et al. Effect of exercise training on ANP receptors. Res Commun Mol Pathol Pharmacol. 2000; 108 (3-4): 227- 235.


26. Hart G. Exercise-induced cardiac hypertrophy: a substrate for sudden death in athletes?. Int J Clin Exp Physiol. 2003; 88 (5): 639- 644.


27. Pluim BM, Zwinderman AH, Van der Laarse A, Van der Wall EE. The athlete's heart. A meta-analysis of cardiac structure and function. Circulation. 2000; 101 (3): 336- 344.


28. Ruskoaho H, Leskinen H, Magga J, Taskinen P, Mantymaa P, Vuolteenaho O, et al. Mechanisms of mechanical load-induced atrial natriuretic peptide secretion: role of endothelin, nitric oxide, and angiotensin II. J Mol Med Clin Appl. 1997; 75 (11-12): 876- 885.


29. Maeda S, Miyauchi T, Iemitsu M, Sugawara J, Nagata Y, Goto K. Resistance exercise training reduces plasma endothelin-1 concentration in healthy young humans. J Cardiovasc Pharmacol. 2004; 44: 443- 446.


30. Van Guilder GP, Westby CM, Greiner JJ, Stauffer BL, DeSouza CA. Endothelin-1 vasoconstrictor tone increases with age in healthy men but can be reduced by regular aerobic exercise. Hypertension. 2007; 50 (2): 403- 409.


31. Emori T, Hirata Y, Imai T, Eguchi S, Kanno K, Marumo F. Cellular mechanism of natriuretic peptides-induced inhibition of endothelin-1 biosynthesis in rat endothelial cells. Int J Endocrinol Metab Disord. 1993; 133 (6): 2474- 2480.


32. Ruskoaho H. Atrial natriuretic peptide: synthesis, release, andmetabolism. Pharmacol Rev. 1992; 44(4): 479-602.


33. Ruskoaho H. Cardiac hormones as diagnostic tools in heart failure. Endocrine Reviews. 2003; 24 (3): 341- 356.


34. Moro C, Crampes F, Sengenes C, De Glisezinski I, Galitzky J, Thalamas C, et al. Atrial natriuretic peptide contributes to physiological control of lipid mobilization in humans. FASEB J. 2004; 18 (7): 908- 910.


35. Sengenes C, Berlan M, De GlisezinskiI, Lafontan M, Galitzky J. Natriuretic peptides: a new lipolytic pathway in human adipocytes. FASEB J. 2000; 14 (10): 1345- 1351.


36. Sengenes C, Bouloumie A, HaunerH, Berlan M, Busse R, Lafontan M, et al. Involvement of a cGMP-dependent pathway in the natriuretic peptide-mediated hormone-sensitive lipase phosphorylation in human adipocytes. J Biol Chem. 2003; 278 (49): 48617- 48626.


37. Laughlin MH, Woodman CR, Schrage WG, Gute D, Price EM. Interval sprint training enhances endothelial function and eNOS content in some arteries that perfuse white gastrocnemius muscle. Eur J Appl Physiol. 2004; 96 (1): 233- 244.