مقایسه اثر ضد باکتریایی عصاره های آبی و هیدروالکلی گیاه رسیده و نارس توت سیاه Morus nigra
محورهای موضوعی : Biotechnological Journal of Environmental Microorganismsفرهاد شریفی 1 , لیلا اسدپور 2 , شهاب شریعتی 3 , علی صالح زاده 4
1 - گروه زیست شناسی ، دانشکده علوم پایه، واحد رشت، دانشگاه آزاد اسلامی ، رشت ، ایران
2 - گروه زیست شناسی، دانشکده علوم پایه، واحد رشت، دانشگاه آزاد اسلامی ، رشت ، ایران
3 - گروه شیمی، دانشکده علوم پایه ، واحد رشت ، دانشگاه آزاد اسلامی، رشت ، ایران
4 - گروه زیست شناسی، دانشکده علوم پایه، واحد رشت، دانشگاه آزاد اسلامی، رشت، ایران
کلید واژه: Herbal extract, Antibacterial resistance, Morus nigra, MIC, MBC,
چکیده مقاله :
The growing issue of microbial resistance to antibiotics has spurred significant efforts to develop new drugs, increasing the interest in plant-based treatments. Morus nigra is a commonly used fruit in medicine. This study aims to examine the antimicrobial properties of both ripe and unripe M. nigra fruits and compare their effects. In this experimental study, the antimicrobial effects of of ripe and unripe M. nigra extracts were evaluated by determining the zone of inhibition, minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC) against Staphylococcus epidermidis, Pseudomonas aeruginosa, Staphylococcus aureus, Streptococcus mutans, and Streptococcus pyogenes. These assessments were conducted using disk diffusion and broth macro-dilution methods. Statistical analysis was carried out using the ANOVA test with SPSS software. The antimicrobial effects of the unripe M. nigra fruit extracts with the MIC values of 0.78-1.5 mg/mL were stronger than ripe fruit extract against all tested bacteria. Also that the tested extracts were more effective against Gram-positive strains than Gram-negative strains Additionally, the antimicrobial properties of the unripe M. nigra extract were comparable to those of selected antibiotics including gentamicin. It can be concluded that unripe fruit exhibits stronger antimicrobial effects than ripe M. nigra fruit. This difference could be attributed to the variation in active compounds that are present at different stages of the fruit's development.
The growing issue of microbial resistance to antibiotics has spurred significant efforts to develop new drugs, increasing the interest in plant-based treatments. Morus nigra is a commonly used fruit in medicine. This study aims to examine the antimicrobial properties of both ripe and unripe M. nigra fruits and compare their effects. In this experimental study, the antimicrobial effects of of ripe and unripe M. nigra extracts were evaluated by determining the zone of inhibition, minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC) against Staphylococcus epidermidis, Pseudomonas aeruginosa, Staphylococcus aureus, Streptococcus mutans, and Streptococcus pyogenes. These assessments were conducted using disk diffusion and broth macro-dilution methods. Statistical analysis was carried out using the ANOVA test with SPSS software. The antimicrobial effects of the unripe M. nigra fruit extracts with the MIC values of 0.78-1.5 mg/mL were stronger than ripe fruit extract against all tested bacteria. Also that the tested extracts were more effective against Gram-positive strains than Gram-negative strains Additionally, the antimicrobial properties of the unripe M. nigra extract were comparable to those of selected antibiotics including gentamicin. It can be concluded that unripe fruit exhibits stronger antimicrobial effects than ripe M. nigra fruit. This difference could be attributed to the variation in active compounds that are present at different stages of the fruit's development.
Ahmad Nejhad, A., Alizadeh Behbahani, B., Hojjati, M., Vasiee, A., & Mehrnia, M. A. (2023). Identification of phytochemical, antioxidant, anticancer and antimicrobial potential of Calotropis procera leaf aqueous extract. Scientific Reports, 13(1), 14716. https://doi.org/10.1038/s41598-023-42086-1
Ahmad, R., & Ahmed, A. (2016). Evaluation of antibacterial activity of Morus nigra & Citrus limon. Journal of Ayurvedic and Herbal Medicine, 2(6), 213-217.
Atanasov, A. G., Zotchev, S. B., Dirsch, V. M., & Supuran, C. T. (2021). Natural products in drug discovery: advances and opportunities. Nature reviews Drug discovery, 20(3), 200-216.
Behl, T., & Kotwani, A. (2017). Chinese herbal drugs for the treatment of diabetic retinopathy. Journal of Pharmacy and Pharmacology, 69(3), 223-235. https://doi.org/10.1111/jphp.12683
Budiman, A., Aulifa, D. L., Kusuma, A. S. W., & Sulastri, A. (2017). Antibacterial and antioxidant activity of black mulberry (Morus nigra L.) extract for acne treatment. Pharmacognosy Journal, 9(5).
de Pádua Lúcio, K., Rabelo, A. C. S., Araújo, C. M., Brandão, G. C., de Souza, G. H. B., da Silva, R. G., de Souza, D. M. S., Talvani, A., Bezerra, F. S., Cruz Calsavara, A. J., & Costa, D. C. (2018). Anti-Inflammatory and Antioxidant Properties of Black Mulberry (Morus nigra L.) in a Model of LPS-Induced Sepsis. Oxidative Medicine and Cellular Longevity, 2018, 5048031. https://doi.org/10.1155/2018/5048031
Farshadfar, C., Mollica, A., Rafii, F., Noorbakhsh, A., Nikzad, M., Seyedi, S. H., Abdi, F., Verki, S. A., & Mirzaie, S. (2020). Novel potential inhibitor discovery against tyrosyl-tRNA synthetase from Staphylococcus aureus by virtual screening, molecular dynamics, MMPBSA and QMMM simulations. Molecular Simulation, 46(7), 507-520.
He, Y., Yu, J., Song, Z., Tang, Z., Duan, J.-A., Zhu, H., Liu, H., Zhou, J., & Cao, Z. (2024). Anti-oxidant effects of herbal residue from Shengxuebao mixture on heat-stressed New Zealand rabbits. Journal of Thermal Biology, 119, 103752.
Issa, N. K., & Abd-Aljabar, R. S. (2017). Phytochemical analysis, antioxidant (DNA Nicking Assay) and antibacterial activities of Morus nigra L. fruits secondary metabolites. Science Journal of University of Zakho, 5(2), 198-204.
Kakouri, E., Daferera, D., Trigas, P., Charalambous, D., Pantelidou, M., Tarantilis, P. A., & Kanakis, C. D. (2023). Comparative Study of the Antibacterial Activity, Total Phenolic and Total Flavonoid Content of Nine Hypericum Species Grown in Greece. Applied Sciences, 13(5), 3305. https://www.mdpi.com/2076-3417/13/5/3305
Kim, N.-N., Kim, W. J., & Kang, S.-S. (2019). Anti-biofilm effect of crude bacteriocin derived from Lactobacillus brevis DF01 on Escherichia coli and Salmonella Typhimurium. Food control, 98, 274-280.
Kumar, A., Kumari, M., Mazumdar, R. S., & Dhewa, T. (2015). In vitro antibacterial activity of ethanolic extracts of Viola serpens and Morus nigra against pathogens isolated from patients suffering from jaundice. World J. Pharm. Res, 4, 889-898.
Lee, J.-W., Kim, E.-N., & Jeong, G.-S. (2024). Anti-Inflammatory Herbal Extracts and Their Drug Discovery Perspective in Atopic Dermatitis. Biomolecules & Therapeutics, 32(1), 25.
Machado, A., Zamora-Mendoza, L., Alexis, F., & Álvarez-Suarez, J. M. (2023). Use of Plant Extracts, Bee-Derived Products, and Probiotic-Related Applications to Fight Multidrug-Resistant Pathogens in the Post-Antibiotic Era. Future Pharmacology, 3(3), 535-567. https://www.mdpi.com/2673-9879/3/3/34
Noorbakhsh, A., Hosseininezhadian Koushki, E., Farshadfar, C., & Ardalan, N. (2022). Designing a natural inhibitor against human kynurenine aminotransferase type II and a comparison with PF-04859989: a computational effort against schizophrenia. Journal of Biomolecular Structure and Dynamics, 40(15), 7038-7051.
Radünz, M., da Trindade, M. L. M., Camargo, T. M., Radünz, A. L., Borges, C. D., Gandra, E. A., & Helbig, E. (2019). Antimicrobial and antioxidant activity of unencapsulated and encapsulated clove (Syzygium aromaticum, L.) essential oil. Food chemistry, 276, 180-186.
Rahimi, A., Razmkhah, K., Mehrnia, M., Mohamadnia, A., Sahebjamee, H., Salehi, S., Asl, E. A., Tahmasebi, H., Shandiz, S. A. S., Davouodbeglou, F., Ghasemi, S., Ardalan, N., & Kordkandi, Z. G. (2016). Molecular docking and binding study of harpagoside and harpagide as novel anti-inflammatory and anti-analgesic compound from Harpagophytum procumbens based on their interactions with COX-2 enzyme. Asian Pacific Journal of Tropical Disease, 6(3), 227-231. https://doi.org/https://doi.org/10.1016/S2222-1808(15)61019-2
Rani, J., Dhull, S. B., Rose, P. K., & Kidwai, M. K. (2024). Drug-induced liver injury and anti-hepatotoxic effect of herbal compounds: a metabolic mechanism perspective. Phytomedicine, 122, 155142.
Sharifee, F., Asadpour, L., Shariati, S., & Salehzadeh, A. (2022). Evaluation of Antibacterial Effect of Aqueous, Hydro-Alcoholic and Alcoholic Extracts of Morus nigra on Gram-Positive and Gram-Negative Bacteria. Journal of Advances in Environmental Health Research, 10(3), 225-234.
Sharma, A., Sharma, L., Nandy, S. K., Payal, N., Yadav, S., Vargas-De-La-Cruz, C., Anwer, M. K., Khan, H., Behl, T., & Bungau, S. G. (2023). Molecular Aspects and Therapeutic Implications of Herbal Compounds Targeting Different Types of Cancer. Molecules, 28(2), 750. https://www.mdpi.com/1420-3049/28/2/750
Shayan, S., Jamaran, S., Askandar, R. H., Rahimi, A., Elahi, A., Farshadfar, C., & Ardalan, N. (2021). The SARS-Cov-2 Proliferation Blocked by a Novel and Potent Main Protease Inhibitor via Computer-aided Drug Design. Iran J Pharm Res, 20(3), 399-418. https://doi.org/10.22037/ijpr.2021.114846.15061
Sherafatizangeneh, M., Farshadfar, C., Mojahed, N., Noorbakhsh, A., & Ardalan, N. (2022). Blockage of the Monoamine Oxidase by a Natural Compound to Overcome Parkinson’s Disease via Computational Biology. Journal of Computational Biophysics and Chemistry, 21(03), 373-387. https://doi.org/10.1142/s2737416522500156
Skrovankova, S., Ercisli, S., Ozkan, G., Ilhan, G., Sagbas, H. I., Karatas, N., Jurikova, T., & Mlcek, J. (2022). Diversity of Phytochemical and Antioxidant Characteristics of Black Mulberry (Morus nigra L.) Fruits from Turkey. Antioxidants, 11(7), 1339. https://www.mdpi.com/2076-3921/11/7/1339
Wang, E., Wang, N., Zou, Y., Fahim, M., Zhou, Y., Yang, H., Liu, Y., & Li, H. (2022). Black mulberry (Morus nigra) fruit extract alleviated AD-Like symptoms induced by toxic Aβ protein in transgenic Caenorhabditis elegans via insulin DAF-16 signaling pathway. Food Research International, 160, 111696. https://doi.org/https://doi.org/10.1016/j.foodres.2022.111696
Wang, F., Wei, F., Song, C., Jiang, B., Tian, S., Yi, J., Yu, C., Song, Z., Sun, L., & Bao, Y. (2017). Dodartia orientalis L. essential oil exerts antibacterial activity by mechanisms of disrupting cell structure and resisting biofilm. Industrial Crops and Products, 109, 358-366.
Zhao, M., Bai, J., Bu, X., Tang, Y., Han, W., Li, D., Wang, L., Yang, Y., & Xu, Y. (2021). Microwave-assisted aqueous two-phase extraction of phenolic compounds from Ribes nigrum L. and its antibacterial effect on foodborne pathogens. Food control, 119, 107449.
