Investigating the relationship between the activities of antioxidant enzymes and genetic Parameters under drought stress conditions of durum wheat genotypes (Triticum durum Desf).
محورهای موضوعی : Genetics
Hasan Bigonah Hamlabad
1
,
Ibrahim Azizov
2
,
Mostafa Valizadeh
3
1 - Department of Plant Biology, Faculty of Agriculture, Ardabil Branch, , Ardabil, Iran.
2 - Institute of Molecular Biology and Biotechnologies of Azerbaijan National Academy of Sciences University, Baku, Azerbaijan
3 - Department of Plant Breeding and Biotechnology, Faculty of Agriculture, University of Tabriz, Tabriz, Iran
کلید واژه: Diallel cross, GCA, SCA, antioxidants enzymes, correlation analysis, drought stress.,
چکیده مقاله :
Drought stress and limited water at the reproductive stage (terminal phase of wheat growth) significantly impact wheat productivity. This study examines terminal drought effects on water relations in wheat. Under drought stress, plants experience hyperosmotic stress at the cellular level, leading to ROS accumulation and oxidative stress, which can impair cell integrity. Plants counter this oxidative damage with a range of enzymatic and non-enzymatic antioxidants to maintain ROS balance. An experiment was conducted using a randomized complete block design with three replicates in both stressed and non-stressed conditions across six durum wheat (Triticum durum) cultivars. Combining abilities for yield, drought tolerance, and quality traits were estimated using a 6 x 6 half-diallel mating design. F1 seeds and parental varieties were evaluated under terminal drought stress, measuring antioxidant enzymes (catalase, ascorbate peroxidase, superoxide dismutase) and grain yield. Results showed that the additive-dominance model was significant for all enzyme traits at the 1% level, with both additive and dominance effects observed. Broad-sense heritability (GCA) was significant at 1% for antioxidant traits and at 5% for catalase; narrow-sense heritability (SCA) was significant at 1% for grain yield. Correlation analysis revealed that antioxidant factors and grain yield were significantly correlated at p < 1% under drought. In conclusion, drought stress enhances the activity of antioxidant enzymes, which plays a crucial role in maintaining yield in durum wheat under terminal drought conditions. Antioxidant enzyme activity is a useful screening tool for drought-resistant genotypes.
Drought stress and limited water at the reproductive stage (terminal phase of wheat growth) significantly impact wheat productivity. This study examines terminal drought effects on water relations in wheat. Under drought stress, plants experience hyperosmotic stress at the cellular level, leading to ROS accumulation and oxidative stress, which can impair cell integrity. Plants counter this oxidative damage with a range of enzymatic and non-enzymatic antioxidants to maintain ROS balance. An experiment was conducted using a randomized complete block design with three replicates in both stressed and non-stressed conditions across six durum wheat (Triticum durum) cultivars. Combining abilities for yield, drought tolerance, and quality traits were estimated using a 6 x 6 half-diallel mating design. F1 seeds and parental varieties were evaluated under terminal drought stress, measuring antioxidant enzymes (catalase, ascorbate peroxidase, superoxide dismutase) and grain yield. Results showed that the additive-dominance model was significant for all enzyme traits at the 1% level, with both additive and dominance effects observed. Broad-sense heritability (GCA) was significant at 1% for antioxidant traits and at 5% for catalase; narrow-sense heritability (SCA) was significant at 1% for grain yield. Correlation analysis revealed that antioxidant factors and grain yield were significantly correlated at p < 1% under drought. In conclusion, drought stress enhances the activity of antioxidant enzymes, which plays a crucial role in maintaining yield in durum wheat under terminal drought conditions. Antioxidant enzyme activity is a useful screening tool for drought-resistant genotypes.
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Apel, K. and H. Hirt. 2004. Reactive oxygen species: metabolism, oxidative stress, and signal transduction. Annu Rev Plant Biol, 55, (1) 373-399.
Change, B. and A. Maehly. 1955. Assay of catalases and peroxidase. Methods Enzymol, 2, 764-775.
Chaudhari, G. R., D. Patel, A. Kalola and S. Kumar. 2023. Use of graphical and numerical approaches for diallel analysis of grain yield and its attributes in bread wheat (Triticum aestivum L.) under varying environmental conditions. Agriculture, 13, (1) 171.
Colasuonno, P., I. Marcotuli, A. Gadaleta and J. M. Soriano. 2021. From genetic maps to QTL cloning: an overview for durum wheat. Plants, 10, (2) 315.
Dumanović, J., E. Nepovimova, M. Natić, K. Kuča and V. Jaćević. 2021. The significance of reactive oxygen species and antioxidant defense system in plants: A concise overview. Frontiers in plant science, 11, 552969.
El Haddad, N., H. Choukri, M. E. Ghanem, A. Smouni, R. Mentag, K. Rajendran, K. Hejjaoui, F. Maalouf and S. Kumar. 2021. High-temperature and drought stress effects on growth, yield and nutritional quality with transpiration response to vapor pressure deficit in lentil. Plants, 11, (1) 95.
Giannopolitis, C. N. and S. K. Ries. 1977. Superoxide dismutases: I. Occurrence in higher plants. Plant physiology, 59, (2) 309-314.
Griffing, B. 1956. Concept of general and specific combining ability in relation to diallel crossing systems. Australian journal of biological sciences, 9, (4) 463-493.
Huseynova, I. M., D. R. Aliyeva and J. A. Aliyev. 2014. Subcellular localization and responses of superoxide dismutase isoforms in local wheat varieties subjected to continuous soil drought. Plant Physiology and Biochemistry, 81, 54-60.
Huseynova, I. M., D. R. Aliyeva, A. C. Mammadov and J. A. Aliyev. 2015. Hydrogen peroxide generation and antioxidant enzyme activities in the leaves and roots of wheat cultivars subjected to long-term soil drought stress. Photosynthesis research, 125, 279-289.
Hussein, M. and M. M. Zaater. 2024. Estimates of genetic parameters, combining abilities and heterosis for some genotypes of bread wheat. Journal of Agricultural Chemistry and Biotechnology, 15, (7) 87-92.
Laus, M. N., M. A. De Santis, Z. Flagella and M. Soccio. 2021. Changes in antioxidant defence system in durum wheat under hyperosmotic stress: A concise overview. Plants, 11, (1) 98.
Li, H., C. Testerink and Y. Zhang. 2021. How roots and shoots communicate through stressful times. Trends in plant science, 26, (9) 940-952.
Marcotuli, I., P. Colasuonno, Y. S. Hsieh, G. B. Fincher and A. Gadaleta. 2020. Non-starch polysaccharides in durum wheat: a review. International journal of molecular sciences, 21, (8) 2933.
Mather, K. and J. Jinks. 1971. Biometrical Genetics. Chapman and Hall Ltd. New Fetter Lane, 14-19.
Mehdy, M. C. 1994. Active oxygen species in plant defense against pathogens. Plant physiology, 105, (2) 467.
Mishra, A. K., R. Rai and S. Agrawal. 2013. Individual and interactive effects of elevated carbon dioxide and ozone on tropical wheat (Triticum aestivum L.) cultivars with special emphasis on ROS generation and activation of antioxidant defence system. Indian J Biochem Biophys, 50, (2) 139-149.
Mishra, N., C. Jiang, L. Chen, A. Paul, A. Chatterjee and G. Shen. 2023. Achieving abiotic stress tolerance in plants through antioxidative defense mechanisms. Frontiers in Plant Science, 14, 1110622.
Mittler, R., S. I. Zandalinas, Y. Fichman and F. Van Breusegem. 2022. Reactive oxygen species signalling in plant stress responses. Nature reviews Molecular cell biology, 23, (10) 663-679.
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1405
Investigating the relationship between the activities of antioxidant enzymes and genetic Parameters under drought stress conditions of durum wheat genotypes (Triticum durum Desf).
Hasan Bigonah Hamlabad*1,Ibrahim Azizov2 ,Mostafa Valizadeh3
1. Department of Plant Biology, Faculty of Agriculture, Ardabil Branch, Islamic Azad University, Ardabil, Iran.
2. Institute of Molecular Biology and Biotechnologies of Azerbaijan National Academy of Sciences University, Baku, Azerbaijan.
3.Department of Plant Breeding and Biotechnology, Faculty of Agriculture, University of Tabriz, Tabriz, Iran.
_______________________________________________________________________________
Abstract
Drought stress and limited water at the reproductive stage (terminal phase of wheat growth) significantly impact wheat productivity. This study examines terminal drought effects on water relations in wheat. Under drought stress, plants experience hyperosmotic stress at the cellular level, leading to ROS accumulation and oxidative stress, which can impair cell integrity. Plants counter this oxidative damage with a range of enzymatic and non-enzymatic antioxidants to maintain ROS balance. An experiment was conducted using a randomized complete block design with three replicates in both stressed and non-stressed conditions across six durum wheat (Triticum durum) cultivars. Combining abilities for yield, drought tolerance, and quality traits were estimated using a 6 x 6 half-diallel mating design. F1 seeds and parental varieties were evaluated under terminal drought stress, measuring antioxidant enzymes (catalase, ascorbate peroxidase, superoxide dismutase) and grain yield. Results showed that the additive-dominance model was significant for all enzyme traits at the 1% level, with both additive and dominance effects observed. Broad-sense heritability (GCA) was significant at 1% for antioxidant traits and at 5% for catalase; narrow-sense heritability (SCA) was significant at 1% for grain yield. Correlation analysis revealed that antioxidant factors and grain yield were significantly correlated at p < 1% under drought. In conclusion, drought stress enhances the activity of antioxidant enzymes, which plays a crucial role in maintaining yield in durum wheat under terminal drought conditions. Antioxidant enzyme activity is a useful screening tool for drought-resistant genotypes.
Keywords: Diallel cross, GCA, SCA, antioxidants enzymes, correlation analysis, drought stress.
Bigonah Hamlabad H., I. Azizov, M. Valizadeh.2024. Investigating the relationship between the activities of antioxidant enzymes and genetic Parameters under drought stress conditions of durum wheat genotypes (Triticum durum Desf). Iranian Journal of Plant Physiology 14 (4), 5297- 5305.
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____________________________________ * Corresponding Author E-mail Address: hassbg32@gmail.com Received: August, 2024 Accepted: September, 2024
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Durum wheat (Triticum turgidum L. subsp. durum Desf.) is one of the most commonly cultivated wheat species worldwide, accounting for nearly 5% of global wheat production (Colasuonno et al., 2021). The EU is the primary producer, with Italy leading in European durum wheat production, followed by France and Turkey. Durum wheat is also cultivated in the northern United States, Canada, Mexico, and sub-Saharan Africa, with most of its production occurring in countries around the Mediterranean basin. Other smaller cultivation areas include Russia, Kazakhstan, Australia, India, and Argentina. This species is a key commodity globally, as its grain is used to produce many foods, with significant variation across producing countries (Laus et al., 2021). Pasta is the most popular durum-based product worldwide, while couscous and bulgur are common in North Africa and the Middle East, respectively. Durum wheat bread is also traditionally important in Southern Italy, Spain, Turkey, and other Mediterranean regions.
Durum wheat grain is a valuable food source with a significant role in the human diet, providing carbohydrates (70.2%), proteins (12.2%), lipids (1.9%), fiber (1.6%), and minerals (1.6%). Whole grains also contain bioactive compounds beneficial for health, including high levels of antioxidants such as carotenoids and polyphenols, along with vitamins, sodium, potassium, calcium, and magnesium. In Mediterranean regions, durum wheat is mainly grown under rain-fed conditions, often encountering drought stress that hampers yield (Marcotuli et al., 2020). Recent studies show complex interactions between membrane transport proteins and ROS in plants, where ROS signaling can activate membrane transporters for substance transport (Li et al., 2021). This transport promotes metabolic responses that enhance antioxidant enzyme activity, scavenge excess ROS, and improve stress tolerance (Yuan et al., 2024).
Under abiotic stress, ROS accumulate in various forms (¹O₂, O₂•−, H₂O₂, and •OH) in the cytosol and plant organelles. Excess ROS can disrupt cell homeostasis, damage lipids and DNA, and lead to cell apoptosis. Reducing excess ROS is crucial for stress resistance, achieved by inhibiting ROS production and promoting ROS catabolism. ROS signaling activates membrane transporters, creating a complex system network (Mittler et al., 2022). Abiotic stresses disrupt cellular biochemistry, producing excessive ROS (Apel and Hirt, 2004). ROS play dual roles in plant growth, acting as signaling molecules and potential oxidative agents that damage plant tissues (Mishra et al., 2023).
Changes in antioxidant enzyme expression or activity have been noted in response to oxidative stress across species (Rajput et al., 2021). Overexpression of antioxidant enzymes is a potential stress biomarker, with enhanced enzyme activity linked to improved stress tolerance (Dumanović et al., 2021). Durum wheat plants with varying drought tolerance exhibit different antioxidant enzyme activities and molecule levels under stress. A pattern of changes in antioxidant enzymes under hyperosmotic stress has been suggested for both sensitive and tolerant durum wheat genotypes (Ami et al., 2020; Huseynova et al., 2014; Huseynova et al., 2015; Sheng et al., 2020). The correlation between catalase (CAT), superoxide dismutase (SOD), ascorbate peroxidase (APX) activities, root and shoot dry weights, and stress tolerance index suggests that biomass and enzymatic antioxidant parameters could be reliable markers for screening wheat genotypes for water stress tolerance during early growth stages (Pour-Aboughadareh et al., 2020).
Alterations in SOD, APX, and CAT activity and ROS concentration have been observed in wheat plants in both field and laboratory (Huseynova et al., 2014; Mishra et al., 2013; Samineni et al., 2022).
The aim of this study was to determine antioxidant enzyme activity in durum wheat genotypes under drought conditions and examine their relationship with productivity.
Materials and Methods
Table 1 Analysis of varians in normal irrigation for grain 6 durum cultivars and antioxidant enzymes attributes.
Ns: non Significant mM/g fW= milimolar per resh wiegh of tissue Unit/g FW=Enzyme activity per fresh wiegh
NS: non-Significant **: Significant at p < 0.01 *: Significant at p < 0.05 mM/g FW= milimolar per fresh wiegh of tissue , Unit/g FW=Enzyme activity per g fresh wiegh
Table 3 Combined analysis of variance for antioxidant enzymes attributes in 6 durum wheat.
Ns: non-Significant **: Significant at p < 0.01 *: Significant at p < 0.05 mM/g FW= milimolar per g fresh wiegh of tissue , Unit/g FW=Enzyme activity per g fresh wiegh
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Enzyme activity for ascorbate peroxidase was measured using the Sayram method (Naz et al., 2022; Sairam et al., 1998), while catalase and superoxide dismutase activity were assessed by the methods of Gyannopolities and Ries (1977), with catalase extracted using the Chance and Maehly method (1955). The units for ascorbate peroxidase and catalase activity were expressed as millimoles per gram of fresh weight of durum wheat tissue, while superoxide dismutase activity was calculated per gram of tissue. Statistical analysis using diallel analysis was performed with Griffing’s method (Griffing, 1956), and genetic parameters for the half diallel cross were estimated using the Singh method ((Singh and Singh, 1984). Broad-sense heritability (H²b), narrow-sense heritability (H²n), and average degree of dominance (D) were calculated according to Mather and Jinks (1971).
The diallel cross provided valuable information. Antioxidant enzymes are regarded as indicators of drought tolerance in wheat, but few studies have examined their genetic basis (Chaudhari et al., 2023; Hussein and Zaater, 2024; Seied-Khamesi et al., 2022; Shahbazi et al., 2018). Common statistical software, including SPSS, MSTATC, SAS, EXCEL, and Dial98, was used for analysis of variance and mean comparisons in this project.
Table 4 Means comparison of studied traits in 6 durum wheat cultivars.
Ascorbate peroxidase (APO), Catalase (CAT), Superoxide dismutase (SOD), grain yield kg/hectare(GY)
Table 5 Correlation Analysis by Pearson method in Normal Condition.
** Correlation is Significant at p < 0.01 * Correlation is Significant at p < 0.05
Table 6 Correlation Analysis by Pearson method in Stress Condition.
** Correlation is Significant at p < 0.01 * Correlation is Significant at p < 0.05
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According to variance analysis, drought stress showed a significant difference (P<1%), while under normal conditions, genotype effects were not significant (Table 1). This finding indicates that water stress conditions had a significant impact on genotype performance. Block effects were not significant, showing that the blocks in the experiment had balanced yield outcomes (Table 2). Combined analysis revealed significant differences (P<1%) in yield across different cultivars (Table 3). Yield, superoxide dismutase, catalase, and ascorbate peroxidase showed significant effects at the 1% level for genotype effects, indicating that antioxidant activity exhibited varied responses in durum wheat varieties. All sources of variation, including superoxide dismutase, catalase, and ascorbate peroxidase, showed significant interaction effects between genotype and environment (Table 3).
Therefore, Duncan’s test was used to achieve detailed results in examining cultivar yield. The Omrabi-5 durum wheat cultivar was in Group A under drought conditions, while other cultivars exhibited different yields under these conditions (Table 4). Therefore, Omrabi-5 was identified as the most resistant variety in drought conditions, while Fadda was recognized as the most sensitive.
In the correlation analysis of grain yield characteristics and antioxidant enzymes using Pearson's method, under normal conditions, enzyme activity did not show a positive, significant
Table 7 Estimates of genetic parameters in half-diallel.
**: Significant at p < 0.01 probability levels, respectively; D: Additive variance, H1: Uncorrected dominance variances, H2: Corrected dominance variances, F: Additive-dominance covariance, Sqr(H1/D): Average degree of dominance, (kd+kr)kd: Proportion of dominant genes, D/(D+E): Heritability by parents, H2b: Heritability for diallel in a broad sense, H2n: Heritability for diallel in a narrow sense, Mp: Mean of Parents, Mf1: Mean of F1s, Vp: Var. of Parents, Vf1: Var. of F1s, Ep: Env. Var. from Parents, Ef1: Env. Var. from F1s
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Additive variance parameters (D) and corrected dominance variance (H1) as well as uncorrected dominance variance (H2) were significant at the 1% level in antioxidant enzyme activity, including superoxide dismutase and ascorbate peroxidase (Table 7). The additive-dominance covariance index showed a significant effect at the 1% level for superoxide dismutase, ascorbate peroxidase, and grain yield. Environmental variance parameters of the parents (Ep) were significant for all traits at the 1% probability level (Table 7). Heritability for diallel in a broad sense (GCA) and in a narrow sense (SCA) was calculated using Griffing’s method (Table 8). Broad-sense heritability (GCA) was significant at 1% for antioxidant enzymes, superoxide dismutase, and ascorbate peroxidase, and at the 5% level for catalase activity. Narrow-sense heritability (SCA) was significant at 1% for grain yield.
Discussion
Based on the results, it was inferred that the additive-dominance model can adequately fit most characters. In the study by Seied-Khamesi and coworkers (Seied-Khamesi et al., 2022), additive genetic effects played a significant role in phenotypic variations of the bread wheat genotypes. In wheat plants, both SCA and GCA effects were significant for stem reserve remobilization (Hussein and Zaater, 2024). Generally, the traits showed high antioxidant enzyme levels, indicating that most of the
Table 8 General combining ability (GCA) and specific combining ability (SCA) mean squares for evaluated traits from a 6 × 6 half-diallel cross in stress conditions durum wheat genotypes.
*, **: Significant at p < 0.05 and p < 0.01 percent probability levels, respectively; GCA: General combining ability, SCA: Specific combining ability |
The magnitude of the average degree of dominance indicated that, for plant resistance to drought, additive effects are more significant. Grain yield was significant at the 1% probability level for narrow-sense heritability (SCA), which showed the greater role of dominance effect in controlling drought resistance parameters. In Griffing's model, based on diallel in a broad sense (GCA), grain yield was not significant, indicating a greater role of additive gene effects in controlling traits. Based on this study's findings, low heterosis
was estimated across all traits, which is typical in self-pollinating crops. Finally, based on GCA effects, the parents ((Hussein and Zaater, 2024; Seied-Khamesi et al., 2022; Shahbazi et al., 2018) possessed favorable alleles for drought resistance in wheat plants. These parents may produce offspring with improved genotypes under terminal drought conditions, and the findings showed the dominance effects higher influence on trait control.
The results showed that increased CAT, SOD, and ascorbate peroxidase activities were observed under drought stress, with this enhancement attributed to the antioxidative defense mechanisms contributing to yield. Mehdy (1994) found that intracellular free radical generation and drought stress were main factors causing plant damage. Some researchers believed that antioxidant enzyme activity increased in durum wheat under drought stress (Ami, et al. 2020). In this experiment, CAT, SOD, and ascorbate peroxidase activities in the drought-sensitive variety started to decrease at the end of the season under drought stress. We hypothesized that these enzyme activities represent a response mechanism of durum wheat cultivars to reduce H₂O₂ levels under drought stress during the final irrigation stage.
The results showed that in drought stress conditions during the last growth stage, antioxidant enzyme activity significantly increases. In drought-resistant genotypes, this enzyme activity increases under drought conditions, resulting in less yield reduction compared to drought-sensitive cultivars, where yield decreases more sharply in the final stage. The relationship between enzyme activity and the antioxidant defense system in drought conditions showed a positive and significant correlation in this research. The grain yield reduction in drought-resistant varieties was less than that in sensitive varieties under drought conditions. For durum wheat varieties subjected to drought in the last stage, the antioxidant defense mechanism quickly mitigates oxygen free radicals. The peak H₂O₂ accumulation was significantly higher in drought-sensitive cultivars than in drought-tolerant ones. In this study, we observed that enzyme activity in resistant genotypes peaked 12 days after drought stress, then rapidly declined, indicating that enzyme activity is regulated by genotype and environment. Under normal conditions, enzyme content did not significantly decrease.
Therefore, we hypothesized that the stress response for eliminating H₂O₂ achieves a dynamic balance between consumption and synthesis at this time. The likely reason was that the higher H₂O₂ levels required higher CAT, SOD, and ascorbate peroxidase activity. Maintaining a high antioxidant enzyme content under severe drought likely contributes to drought resistance. Maintaining high antioxidant capacity to scavenge ROS is associated with increased plant tolerance to various environmental stresses, which our research supports. Antioxidant enzyme activity can be a screening tool for drought-resistant genotypes in durum wheat plants. Our research results align with most studies (El Haddad et al., 2021; Laus et al., 2021; Mishra et al., 2023; Qayyum et al., 2021; Quagliata et al., 2023; Yuan et al., 2024).
Conclusion
Our results indicate that distinct metabolic responses in durum wheat are related to its genetic potential for yield, leading to improved tolerance against drought. The distinct induction of defense system activity under drought conditions was accompanied by a significant decrease in damage indices. Therefore, the degree of tolerance is a specific trait under drought stress, showing a positive and significant correlation between antioxidant activity in the present research. Under drought stress, unlike the continuous increase in antioxidant activity in durum cultivars, its relative increase in the tolerant cultivar did not cause cell damage. Instead, it likely acted as a factor inhibiting the effects of drought stress. The increase in ROS content may affect the tolerance level of the genotypes, so we measured antioxidant activity as one of the important parameters to determine drought tolerance.
According to the results of performance reviews and related traits under both water stress and normal conditions, as well as consideration of testing and variance analysis in correlation design, and review of treatments under water stress and normal conditions with respect to genotype effects, significant effects were observed in both genotype and interaction effects. We conclude that the present findings are consistent with the results of most studies. It was concluded that drought stress plays an effective role in the activity of Superoxide Dismutase, Catalase, and Ascorbate Peroxidase antioxidant enzymes. The activity of these antioxidant enzymes plays a vital role in preventing yield reduction in durum wheat genotypes under end-of-season drought conditions. Antioxidant enzyme activity can therefore be used as a screening tool for drought-resistant genotypes of durum wheat in plant breeding programs.
References
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Apel, K. and H. Hirt. 2004. Reactive oxygen species: metabolism, oxidative stress, and signal transduction. Annu Rev Plant Biol, 55, (1) 373-399.
Change, B. and A. Maehly. 1955. Assay of catalases and peroxidase. Methods Enzymol, 2, 764-775.
Chaudhari, G. R., D. Patel, A. Kalola and S. Kumar. 2023. Use of graphical and numerical approaches for diallel analysis of grain yield and its attributes in bread wheat (Triticum aestivum L.) under varying environmental conditions. Agriculture, 13, (1) 171.
Colasuonno, P., I. Marcotuli, A. Gadaleta and J. M. Soriano. 2021. From genetic maps to QTL cloning: an overview for durum wheat. Plants, 10, (2) 315.
Dumanović, J., E. Nepovimova, M. Natić, K. Kuča and V. Jaćević. 2021. The significance of reactive oxygen species and antioxidant defense system in plants: A concise overview. Frontiers in plant science, 11, 552969.
El Haddad, N., H. Choukri, M. E. Ghanem, A. Smouni, R. Mentag, K. Rajendran, K. Hejjaoui, F. Maalouf and S. Kumar. 2021. High-temperature and drought stress effects on growth, yield and nutritional quality with transpiration response to vapor pressure deficit in lentil. Plants, 11, (1) 95.
Giannopolitis, C. N. and S. K. Ries. 1977. Superoxide dismutases: I. Occurrence in higher plants. Plant physiology, 59, (2) 309-314.
Griffing, B. 1956. Concept of general and specific combining ability in relation to diallel crossing systems. Australian journal of biological sciences, 9, (4) 463-493.
Huseynova, I. M., D. R. Aliyeva and J. A. Aliyev. 2014. Subcellular localization and responses of superoxide dismutase isoforms in local wheat varieties subjected to continuous soil drought. Plant Physiology and Biochemistry, 81, 54-60.
Huseynova, I. M., D. R. Aliyeva, A. C. Mammadov and J. A. Aliyev. 2015. Hydrogen peroxide generation and antioxidant enzyme activities in the leaves and roots of wheat cultivars subjected to long-term soil drought stress. Photosynthesis research, 125, 279-289.
Hussein, M. and M. M. Zaater. 2024. Estimates of genetic parameters, combining abilities and heterosis for some genotypes of bread wheat. Journal of Agricultural Chemistry and Biotechnology, 15, (7) 87-92.
Laus, M. N., M. A. De Santis, Z. Flagella and M. Soccio. 2021. Changes in antioxidant defence system in durum wheat under hyperosmotic stress: A concise overview. Plants, 11, (1) 98.
Li, H., C. Testerink and Y. Zhang. 2021. How roots and shoots communicate through stressful times. Trends in plant science, 26, (9) 940-952.
Marcotuli, I., P. Colasuonno, Y. S. Hsieh, G. B. Fincher and A. Gadaleta. 2020. Non-starch polysaccharides in durum wheat: a review. International journal of molecular sciences, 21, (8) 2933.
Mather, K. and J. Jinks. 1971. Biometrical Genetics. Chapman and Hall Ltd. New Fetter Lane, 14-19.
Mehdy, M. C. 1994. Active oxygen species in plant defense against pathogens. Plant physiology, 105, (2) 467.
Mishra, A. K., R. Rai and S. Agrawal. 2013. Individual and interactive effects of elevated carbon dioxide and ozone on tropical wheat (Triticum aestivum L.) cultivars with special emphasis on ROS generation and activation of antioxidant defence system. Indian J Biochem Biophys, 50, (2) 139-149.
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