اثر تعاملی یک دوره تمرین هوازی به همراه مصرف عصاره سیر و استویا بر بیان ژن گیرنده‌های انسولین و شبه- پپتید-گلوکاگن-1 در رت های نر ویستار چاق شده با رژیم پر چرب
محورهای موضوعی : تشخیص مولکولی نشانگر های بیوشیمیایی و ژنتیکیرسول عزیزی 1 , رضا رضایی شیرازی 2 , سیدجواد ضیاءالحق 3 , ندا آقایی بهمن بگلو 4
1 - گروه تربیت بدنی و علوم ورزشی، واحد علی آباد کتول، دانشگاه آزاد اسلامی، علی آباد کتول، ایران
2 - گروه تربیت بدنی، واحد علی آباد کتول، دانشگاه آزاد اسلامی، علی آباد کتول، ایران
3 - گروه تربیت بدنی، دانشکده علوم انسانی، دانشگاه آزاد اسلامی واحد شاهرود، شاهرود
4 - گروه تربیت بدنی و علوم ورزشی، واحد علی آباد کتول، دانشگاه آزاد اسلامی، علی آباد کتول، ایران
کلید واژه: ورزش هوازی, عصاره سیر-استویا, بیان ژن انسولین, گلوکاگن, رت ویستار,
چکیده مقاله :
زمینه و هدف: ورزش و عصاره‌های خوراکی در جلوگیری افزایش چاقی و عوارض آن نقش دارد. هدف از این تحقیق بررسی اثر مصرف مکمل تمرین هوازی و مصرف عصاره سیر-استویا بر بیان ژن های انسولین، پپتید-شبه گلوکاگن-1 در رت های ویستاره چاق شده با رژیم پرچرب بود.
مواد و روش ها: در این مطالعه تجربی، تعداد 33 سر موش نر ویستار با وزن 180 تا 220گرم به طور تصادفی در 7 گروه شامل کنترل سالم، چاق، چاق+سیر، چاق+استویا، چاق+ورزش، چاق+ورزش+سیر، چاق+ورزش+استویا تقسیم شدند. رت ها پس از یک دوره 12-18هفته(تحت پروتکل چاقی)براساس شاخص لی به وزن بالای 310 گرم رسیدند. سپس گروه های تجربی به مدت هشت هفته 40 میلی گرم به ازای هرکیلوگرم وزن بدن مکمل سیر دریافت کردند. پروتکل ورزش با 30دقیقه در روز، 8 متر در دقیقه، براساس برنامه 6جلسه در هفته و به مدت هشت هفته اجرا شد. میزان بیان ژن با استفاده از روش Real Time PCR اندازه‌گیری و داده ها به روش تحلیل واریانس یک طرفه و آزمون تعقیبی توکی در سطح معنی داری p-value<0.05 تجزیه و تحلیل گردیدند.
نتایج: بیان ژن انسولین در گروه چاق+ورزش تفاوت معناداری با گروه کنترل دارد(P-Value=0.013<0.05). به بیان بهتر، می‌توان گفت که ورزش سبب کاهش معنادار میزان بیان ژن انسولین در رت‌های نر چاق شده است. همچنین ملاحظه می شود که تفاوت معناداری بین میانگین ژن انسولین گروه چاق+استویا با گروه چاق+ورزش(Sig=0.000 ) و گروه چاق+ورزش+سیر (sig=0.003) وجود دارد. اما نتایج آنالیز واریانس تفاوت معناداری بین میانگین ژن شبه پپتید-گلوکاگن-1 در گروه‌های هفت گانه رت‌های نر نشان نداد(P-Value=0.824>0.05).
نتیجه گیری: چاقی موجب تغییرات چشمگیر بیان ژن های درگیر در حساسیت انسولین دارد و بنظر میرسد فعالیت بدنی هوازی با و بدون سیر و استویا میتواند بر این تغییرات اثر مثبت داشته باشد.
Background & Aim: exercise and edible extracts play a role in preventing the increase of obesity and its complications. The purpose of this research was to investigate the effect of aerobic exercise supplement and garlic-stovia extract on the expression of insulin, glucagon-like peptide-1 genes in Wistare rats fed a high-fat diet.
Materials & Methods: In this experimental study, 33 male Wistar rats were randomly divided into 7 groups including healthy control, obese, obese + garlic, obese + stevia, obese + exercise, obese + exercise + garlic, obese + exercise + stevia. After a period of 12-18 weeks, the rats reached a weight of over 310 grams based on the Lee index. Then, the experimental groups received 40 mg per kilogram of body weight of garlic supplement for eight weeks. The exercise protocol was performed with 30 minutes per day, 8 meters per minute, based on a program of 6 sessions per week for eight weeks. gene expression levels were measured using Time PCR method, and the data were analyzed using one-way analysis of variance and Tukey's post hoc test at a significance level of p-value <0.05.
Results: that the expression of insulin gene in the obese + exercise group is significantly different from the control group (P-Value=0.013<0.05). To put it better, it can be said that exercise caused a significant decrease in insulin gene expression in obese male rats. It can also be seen that there is a significant difference between the average insulin gene of the obese group + stevia with the obese + exercise group (Sig = 0.000) and the obese group + exercise + garlic (sig = 0.003). However, the results of analysis of variance did not show a significant difference between the mean of glucagon-like peptide-1 gene in the seven groups of male rats (P-Value=0.824>0.05).
Conclusion: Obesity causes significant changes in the expression of genes involved in insulin sensitivity, and it seems that aerobic physical activity with and without garlic and stevia can have a positive effect on these changes.
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