بهبود نیمرخ گلیسیمیک متعاقب تمرینات مقاومتی در رت‌های دیابتی با تاکید بر بیان لیپوکالین-2
محورهای موضوعی : فصلنامه زیست شناسی جانوریمحمد پارک‌رای 1 , شهرام سهیلی 2
1 - گروه تربیت بدنی و علوم ورزشی، واحد شهر‌قدس، دانشگاه آزاد اسلامی، تهران، ایران
2 - گروه تربیت بدنی و علوم ورزشی، واحد شهر‌قدس، دانشگاه آزاد اسلامی، تهران، ایران
کلید واژه: تمرین مقاومتی, بیان لیپوکالین 2, مقاومت انسولین, نیمرخ گلیسیمیک, دیابت نوع 2,
چکیده مقاله :
لیپوکالین-2 از مهمترین میانجی‌های التهابی مرتبط با چاقی و موثر در هموستاز گلوکز معرفی شده است. مطالعه حاضر با هدف تعیین اثر تمرینات مقاومتی بر بیان ژن لیپوکالین-2 در بافت چربی زیرپوستی همچنین نیمرخ گلیسیمیک در رت‌های چاق دیابتی بود. 14 سر رت نر ویستار پس از القای دیابت نوع 2 توسط رژیم غذایی پرچرب و تزریق STZ به گروه‌های کنترل و تمرین مقاومتی تقسیم شدند. تمرینات مقاومتی به مدت 6 هفته به تعداد 5 جلسه در هفته در قالب بالا رفتن از نردبان پله‌ای توسط رت‌های گروه مقاومتی انجام گرفت. 48 ساعت پس از آخرین جلسه تمرینی، همه رت‌ها تشریح شدند. بیان لیپوکالین-2، گلوکز، هموگلوبین گلیکوزیله و مقاومت انسولین اندازه‌گیری شدند و توسط آزمون تی مستقل بین دو گروه مقایسه شدند. در مقایسه با گروه کنترل، تمرینات مقاومتی به کاهش معنی‌دار گلوکز (001/0 = p)، هموگلوبین گلیکوزیله (016/0 = p)، مقاومت انسولین (001/0 = p) و بیان لیپوکالین-2 در بافت چربی زیرپوستی (003/0 = p) همچنین افزایش معنی‌دار انسولین سرم (042/0 = p) منجر شد. با استناد به این یافته‌ها، بهبود نیمرخ گلیسیمیک در رت‌های مورد مطالعه را شاید بتوان به کاهش بیان لیپوکالین-2 و کاهش مقاومت انسولین متعاقب تمرینات مقاومتی نسبت داد. درک و آگاهی بیشتر از مکانیسم‌های عهده دار این تغییرات نیازمند مطالعات بیشتر است.
Lipocalin-2 has been introduced as one of the most important inflammatory mediators related to obesity and effective in glucose homeostasis. The purpose of this study was to determine the effect of resistance training on lipocalin 2 gene expression in subcutaneous adipose tissue as well as glycemic profile in obese diabetic rats. 14 male Wistar rats were divided into control and resistance training groups after induction of type 2 diabetes by high-fat diet and STZ injection. Resistance exercises were performed for 6 weeks, 5 sessions per week, in the form of climbing a step ladder by the rats of the resistance group. 48 hours after the last training session, all rats were dissected. Lipocalin 2 gene expression, glucose, glycosylated hemoglobin and insulin resistance were measured and compared between two groups by independent t-test. Compared to the control group, resistance training resulted in a significant decrease in glucose (P = 0.001), glycosylated hemoglobin (P = 0.016), insulin resistance (P = 0.001) and lipocalin 2 gene expression in subcutaneous fat tissue (P = 0.003) and as well as a significant increase in serum insulin (P = 0.042). Based on these findings, the improvement of the glycemic profile in the studied rats may be attributed to the decrease in the expression of lipocalin-2 and insulin resistance following resistance training. Further studies are needed to better understand the mechanisms responsible for these changes.
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