The effect of Lactobacillus casei derived extracellular vesicles on the expression of Toll-like receptor-2 gene

محورهای موضوعی : Food and Health

Maryam Ebrahimi Vargoorani ¹ , Mohammad Hossein Modarressi ² , Elahe Motevaseli ³ , Farzam Vaziri ⁴ , Seyed Davar Siadat ⁵

1 - Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
2 - Department of Medical Genetics, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
3 - Department of Molecular Medicine, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
4 - Department of Mycobacteriology and Pulmonary Research, Pasteur Institute of Iran, Tehran, Iran
5 - Department of Mycobacteriology and Pulmonary Research, Pasteur Institute of Iran, Tehran, Iran

تاریخ دریافت : 1397/03/29 تاریخ پذیرش : 1397/05/27 تاریخ انتشار : 1397/06/10

کلید واژه: Toll-like receptor, Extracellular vesicles, Gene, &lt, i&gt, Lactobacillus casei&lt, /i&gt, ,

چکیده مقاله :

In Gram-positive bacteria, the production of extracellular vesicles has been neglected to date due to the presence of a thick peptidoglycan cell wall. But in recent years more studies have been done on these nanoparticles. The purpose of this study is to show that Lactobacillus casei has the ability to produce extracellular vesicles. Since extracellular vesicles (EVs) are an important agent to mediate the expression of genes. In this scientific research, we have examined to find out the effect of EVs derived from L. casei on the expression of Toll-like receptor 2 gene. The EVs were purified from the conditioned medium of L. casei using ultracentrifugation and confirmed by scanning electron microscopy (SEM). The Caco2 cells were treated with different concentrations of purified extracellular vesicles. The electron microscopy showed spherical vesicles that had an average diameter of 200nm. The extracted protein content was 2.4 in the ultracentrifugation method. It was determined that the extracellular vesicles of this bacterium at concentrations of 150 μg/ml had no significant effect on the expression of Toll-like receptor-2 gene expression in comparison with the control (Sucrose), whereas the expression of this gene in the treatment of EVs at concentrations of 50 and 100 μg/ml decreased. Our result creates a paradigm for future studies of the functional component from gut microbiota as a new possible dietary supplement instead of probiotic.

منابع و مأخذ:

1. Bernardeau  M, Vernoux JP, Henri-Dubernet S, Gueguen M. Safety assessment of dairy microorganisms: The Lactobacillus genus. International Journal of Food Microbiology. 2008;126(3):278-85.


2. Khani SM, Hosseini H, Taheri MR, Nourani MA. Imani Fooladi AJI, Targets AD. Probiotics as an alternative strategy for prevention and treatment of human diseases: a review. Inflammation & Allergy-Drug Targets. 2012;11(2):79-89.


3. Kumar A, Kumar M, Ghosh M, Ganguli AJM, immunology. Modeling in vitro cholesterol reduction in relation to growth of probiotic Lactobacillus casei. Microbiology and Immunology. 2013;57(2):100-10.


4. Sharma M, Devi M. Probiotics: a comprehensive approach toward health foods. Critical Reviews in Food Science and Nutrition. 2014;54(4):537-52.


5. So JS, Lee CG, Kwon HK, Yi HJ, Chae CS, Park JA, et al. Lactobacillus casei potentiates induction of oral tolerance in experimental arthritis. Molecular Immunology. 2008;46(1):172-80.


6. Teanpaisan R, Hintao J, Dahlén G. Oral Lactobacillus species in type 2 diabetic patients living in southern Thailand. Anaerobe. 2009;15(4):160-3.


7. Kim MS, Kim JE, Yoon YS, Kim TH, Seo JG, Chung MJ, et al. Improvement of atopic dermatitis-like skin lesions by IL-4 inhibition of P14 protein isolated from Lactobacillus casei in NC/Nga mice. Applied Microbiology and Biotechnology. 2015;99(17):7089-99.


8. Han DJ, Kim JB, Park SY, Yang MG, Kim H. Growth inhibition of hepatocellular carcinoma Huh7 cells by Lactobacillus casei extract. Yonsei Medical Journal. 2013;54(5):1186-93.


9. Karaca H, Velioglu YS. Effects of ozone treatments on microbial quality and some chemical properties of lettuce, spinach, and parsley. Postharvest Biology and Technology. 2014; 88:46-53.


10. Castillo NA, Perdigón G, de LeBlanc AdM. Oral administration of a probiotic Lactobacillus modulates cytokine production and TLR expression improving the immune response against Salmonella enterica serovar Typhimurium infection in mice. BMC microbiology. 2011;11(1):177.


11. Kulp A, Kuehn MJ. Biological functions and biogenesis of secreted bacterial outer membrane vesicles. Annual Review of Microbiology. 2010;64:163-84.


12. Brown L, Wolf JM, Prados-Rosales R, Casadevall A. Through the wall: extracellular vesicles in Gram-positive bacteria, mycobacteria and fungi. Nature Reviews Microbiology. 2015;13(10):620.


13. Lee EY, Choi DY, Kim DK, Kim JW, Park JO, Kim S, et al. Gram‐positive bacteria produce membrane vesicles: proteomics‐based characterization of Staphylococcus aureus‐derived membrane vesicles. Proteomics. 2009;9(24):5425-36.


14. Chiba M, Watanabe N, Watanabe M, Sakamoto M, Sato A, Fujisaki M, et al. Exosomes derived from SW480 colorectal cancer cells promote cell migration in HepG2 hepatocellular cancer cells via the mitogen-activated protein kinase pathway. International Journal of Oncology. 2016;48(1):305-12.


15. Siadat SD, Naddaf SR, Zangeneh M, Moshiri A, Sadat SM, Ardestani MS, et al. Outer membrane vesicle of Neisseria meningitidis serogroup B as an adjuvant in immunization of rabbit against Neisseria meningitidis serogroup A. African Journal of Microbiology Research. 2011;5(19):3090-5.


16. Cho I, Blaser MJ. The human microbiome: at the interface of health and disease. Nature Reviews Genetics. 2012;13(4):260.


17. Villena J, Kitazawa H. Modulation of intestinal TLR4-inflammatory signaling pathways by probiotic microorganisms: lessons learned from Lactobacillus jensenii TL2937. Frontiers in Immunology. 2014; 4:512.


18. Gurung M, Moon DC, Choi CW, Lee JH, Bae YC, Kim J, et al. Staphylococcus aureus produces membrane-derived vesicles that induce host cell death. PloS one. 2011;6(11): e27958.


19. Joffe LS, Nimrichter L, Rodrigues ML, Del Poeta M. Potential roles of fungal extracellular vesicles during infection. mSphere. 2016;1(4): e00099-16.


20. de Oliviera Nascimento L, Massari P, Wetzler LM. The role of TLR2 in infection and immunity. Frontiers in Immunology. 2012; 3:79.


21. van Bergenhenegouwen J, Kraneveld AD, Rutten L, Kettelarij N, Garssen J, Vos AP. Extracellular vesicles modulate host-microbe responses by altering TLR2 activity and phagocytosis. PloS one. 2014;9(2): e89121.