Preliminary phytochemical analysis and in vitro antioxidant properties of Malaysian ‘Kundang’ (Bouea macrophylla Griffith)
محورهای موضوعی : Phytochemistry: Isolation, Purification, Characterization
1 - Department of Anatomy, Faculty of Medicine, Universiti Kebangsaan Malaysia, Kuala Lumpur, Malaysia
کلید واژه: phytochemical analysis, Secondary metabolites, <i>Bouea macrophylla</i> Griffith, <i>In vitro</i> antioxidant activity,
چکیده مقاله :
The main objective of the present study was to evaluate preliminary phytochemical screening and in vitro antioxidant properties of leaves, ripe and unripe fruit extracts of Malaysian ‘Kundang (Bouea macrophylla Griffith; Family: Anacardiaceae), by using different solvents like water, ethanol, methanol, and hexane. Phytochemical analysis showed the presence of alkaloids, flavonoids, saponins, sterols and triterpenes, total phenol, tannins, and vitamin C. The prepared extracts from the leaves and fruits of the plant were further investigated for their potential antioxidant activity using radical scavenging DPPH (2,2'-diphenyl-2-picrylhydrazyl) technique, which was compared with ascorbic acid, as standard. This study showed that ripe, unripe fruits and leaves of B. macrophylla have higher amounts of various phytochemicals constituents in methanol and aqueous solvents and possess higher amounts of vitamin C. Moreover, aqueous and methanol extracts of leaves, unripe and ripe fruits of B. macrophylla exhibited a remarkable DPPH radical scavenging activity compared with that of the standard. The results revealed that fruit and leaves extracts are a potential source of antioxidants of natural origin.
Amir, M., Mujeeb, M., Khan, A., Ashraf, K., Sharma, D., Aqil, M., 2012. Phytochemical analysis and in vitro antioxidant activity of Uncariagambir. Int. J. Green Pharm. 6, 67-72.
Andina, L., Musfirah, Y., 2017. Total phenolic content of cortex and leaves of Ramania (Bouea macrophylla Griffith) and antioxidant activity assay by DPPH method. Res. J. Pharma. Biol. Chem. Sci. 8(1S), 134-140.
Asif, M., Naqvi, S.A.R., Sherazi, T.A., Ahmad, M., Zahoor, A.F., Shahzad, S.A., Hussain, Z., Mahmood, H., Mahmood, N., 2017. Antioxidant, antibacterial and antiproliferative activities of pumpkin (cucurbit) peel and puree extracts - an in vitro study. Pak. J. Pharm. Sci. 30(4), 1327-1334
Baby, B., Antony, P., Vijayan, R., 2017. Antioxidant and anticancer properties of berries. Crit. Rev. Food Sci. Nutr. 13, 1-17. doi: 10.1080/10408398.2017.
Bhaskaran, S.K., Kannappan, P., 2017. Protective effect of Azolla microphylla on biochemical, histopathological and molecular changes induced by isoproterenol in rats. Biomed. Pharmacother. 89, 473-481.
Clementino, M., Shi, X., Zhang, Z., 2017. Prevention of polyphenols against carcinogenesis induced by environmental carcinogens. J. Environ. Pathol. Toxicol. Oncol. 36(1), 87-98.
De Ancos, B., Sgroppo, S., Plaza, L., Cano, M.P., 2002. Possible nutritional and health-related value promotion in orange juice preserved by high-pressure treatment. J. Sci. Food Agric. 82, 790-796.
Dib, I., Tits, M., Angenot, L., Wauters, J.N., Assaidi, A., Mekhfi, H., Aziz, M., Bnouham, M., Legssyer, A., Frederich, M., Ziyyat, A., 2017. Antihypertensive and vasorelaxant effects of aqueous extract of Artemisia campestris L. from Eastern Morocco. J. Ethnopharmacol. 206, 224-235.
Fernando, W.M.A.D.B., Somaratne, G., Goozee, K.G., Williams, S., Singh, H., Martins, R.N., 2017. Diabetes and Alzheimer's disease: Can tea phytochemicals play a role in prevention? J. Alzheimers Dis.59(2), 481-501. doi: 10.3233/JAD-161200.
Ganesan, K., Letha, N., Nair, S.K.P., Gani, S.B., 2015. Evaluation of phytochemical screening and in vitro antioxidant activity of Cordia Africana Lam. (Family: Boraginaceae), A native African herb. Int. J. Pharma Res. Scholar. 4(4), 188-196.
Ganesan, K., Nair, S.K.P., Azalewor, H.G., Gani, S.B., Letha, N., 2016. Preliminary phytochemical screening and in vitro antioxidant activity of Datura stramonium l. collected from Jimma, South West Ethiopia. Int. J. Pharma Bio Sci. 7(1), 261-266.
Ganesan, K., Sukalingam, K., Xu, B., 2017. Solanum trilobatum L. ameliorate thioacetamide-induced oxidative stress and hepatic damage in albino rats. Antioxidants (Basel). 6(3), pii: E68. doi: 10.3390/antiox6030068.
Ganesan, K., Xu, B., 2017. A critical review on polyphenols and health benefits of black soybeans. Nutrients. 9(5), pii: E455. doi: 10.3390/nu9050455.
Hu, Y.P., Peng, Y.B., Zhang, Y.F., Wang, Y., Yu, W.R., Yao, M., Fu, X.J., 2017. Reactive oxygen species mediated prostaglandin E2 contributes to acute response of epithelial injury. Oxid. Med. Cell Longev. 2017, 4123854.
Hu, Y.X., Zhang, W., Zhang, W., Zhu, Y.D., Ma, G.X., Zhu, N.L., Sun, W., Ma, Z.X., Yu, S.C., Xu, X.D., Chen, K.T., Yang, J.S., 2017. Oleanane triterpene saponins with cardioprotective activity from Clinopodium polycephalum. J. Asian Nat. Prod. Res. 19(7), 697-703.
Hussein, S.R., Marzouk, M.M., Soltan, M.M., Ahmed, E.K., Said, M.M., Hamed, A.R., 2017. Phenolic constituents of Pulicaria undulata (L.) C.A. Mey. Sub Sp. undulata (Asteraceae): Antioxidant protective effects and chemosystematic significances. J. Food Drug. Anal. 25(2), 333-339.
Ibrahim, M.H., Chee Kong, Y., Mohd Zain, N.A. Effect of cadmium and copper exposure on growth, secondary metabolites and antioxidant activity in the medicinal plant Sambung Nyawa (Gynura procumbens (Lour.) Merr). Molecules. 22(10). pii: E1623.
Ikram, E.H.K., Eng, K.H., Jalil, A.M.M., Ismail, A., Idris, S., Azlan, A., Nazri, H.S.M., Diton, N.A.M., Mokhtar, R.A.M., 2009. Antioxidant capacity and total phenolic content of Malaysian underutilized fruits. J. Food Comp. Analys. 22, 388- 393.
Kahl, R., Kappus, H., 1993. Toxicology of the synthetic antioxidants BHA and BHT in comparison with the natural antioxidant vitamin E. Z Lebensm Unters Forsch. 196(4), 329-338.
Kolar, F.R., Gogi, C.L., Khudavand, M.M., Choudhari, M.S., Patil, S.B., 2017. Phytochemical and antioxidant properties of some Cassia species. Nat. Prod. Res. 18, 1-5
Koleva, I.I., Van Beek, T.A., Linssen, J.P.H., de Groot, A., Evstatieva, L.N., 2002. Screening of plant extracts for antioxidant activity: a comparative study on three testing methods. Phytochem. Anal. 13, 8-17.
Kwon, S.J., Hwang, S.J., Jung, Y., Park, H.G., Kim, M.H., Park, Y., Lee, H.J, 2017. A synthetic Nitraria alkaloid, isonitramine protects pancreatic β-cell and attenuates postprandial hyperglycemia. Metabolism 70, 107-115.
Laamech, J., El-Hilaly, J., Fetoui, H., Chtourou, Y., Gouitaa, H., Tahraoui, A., Lyoussi, B., 2017. Berberis vulgaris L. effects on oxidative stress and liver injury in lead-intoxicated mice. J. Complement. Integr. Med. 14(1). pii: /j/jcim.2017.
Letha, N., Ganesan, K., Nair, S.K.P., Azalewor, H.G., Gani, S.B., 2016. Evaluation of in vitro antioxidant activity and phytochemical screening of Croton macrostachyus Hochst. by using different solvent extracts. Am. J. PharmTech. Res. 6(1), 74-85.
Liu, X., Li, M., Tan, S., Wang, C., Fan, S., Huang, C., 2017. Harmine is an inflammatory inhibitor through the suppression of NF-κB signaling. Biochem. Biophys. Res. Commun. 489(3), 332-338.
Mohammadhosseini, M., 2017. The ethnobotanical, phytochemical and pharmacological properties and medicinal applications of essential oils and extracts of different Ziziphora species. Ind. Crops Prod. 105, 164-192.
Mohammadhosseini, M., Sarker, S.D., Akbarzadeh, A., 2017. Chemical composition of the essential oils and extracts of Achillea species and their biological activities: A review. J. Ethnopharmacol. 199, 257-315.
Mohammadhosseini, M., Venditti, A., Sarker, S.D., Nahar, L., Akbarzadeh, A., 2019. The genus Ferula: Ethnobotany, phytochemistry and bioactivities – A review. Ind. Crops Prod. 129, 350-394.
Mohib, M.M., Afnan, K., Paran, T.Z., Khan, S., Sarker, J., Hasan, N., Hasan, I., Sagor, M.A.T, 2017. Beneficial role of citrus fruit polyphenols against hepatic dysfunctions: A review. J. Diet Suppl. 22, 1-29.
Nahm, H.S., Juliani, H.R., Simon, J.E., 2012. Effects of selected synthetic and natural antioxidants on the oxidative stability of Shea Butter (Vitellaria paradoxa sub sp. paradoxa). J. Med. Active Plants 1(2), 69-75.
Nair, S.K.P., Ganesan, K., Azalewor, H.G., Letha, N., Gani, S.B., 2016. Preliminary phytochemical screening and in vitro antioxidant activity of Ethiopian indigenous medicinal plants, Ocimum lamiifolium Hochst. ex Benth and Ocimum basilicum L. Int. J. Pharma. Sci. Drug Res. 8(1): 30-36
Nehete, J., Bhatia, M., Narkhede, M., 2010. In-vitro evaluation of antioxidant activity and phenolic content of Costus speciosus (Koen) J.E. Sm. Iran. J. Pharm. Res. 9(3), 271-277.
Omisore, N.O.A., Adewunmi, C.O., Iwalewa, E.O., Ngadjui, B.T., Adenowo, T.K., Abegaz, B.M., Ojewole, J.A., Watchueng, J., 2005. Antitrichomonal and antioxidant activities of Dorstenia barteri and Dorstenia convexa. Brazilian J. Med. Biol. Res. 38, 1087-1094.
Paech, D., Tracey, M.V., 1955. Modern methods of plant analysis, 4th Ed., Wieland: Springer Verlag, pp. 371-373.
Patil, S.M., Kadam, V.J., Ghosh, R., 2009. In vitro antioxidant activity of methanolic extract of stem bark of Gmelina arborea roxb. (Verbenaceae). Int. J.Pharma Tech Res. 1, 1480-1484.
Rajan, N.S., Bhat, R., 2016. Antioxidant compounds and antioxidant activities in unripe and ripe Kundang fruits (Bouea macrophylla Griffith). Fruits. 71(1), 41-47.
Rajan, N.S., Bhat, R., 2017. Volatile constituents of unripe and ripe Kundang fruits (Bouea macrophylla Griffith). Int. J. Food Prop. 20(8), 1751-1760.
Rajan, N.S., Bhat, R., Karim, A.A., 2014. Preliminary studies on the evaluation of nutritional composition of unripe and ripe ‘Kundang’ fruits (Bouea macrophylla Griffith). Int. Food Res. J. 21, 985-990.
Sarla, S., Prakash, M.A., Apeksha, R., Subhash, C., 2011. Free radical scavenging (DPPH) and ferric reducing ability (FRAP) of Aphanamixispolystachya (Wall) Parker. Int. J. Drug Dev. Res. 3, 271-274.
Schillacia, C., Nepravishtaa, R., Bellomariaa, A., 2013. Antioxidants in food and pharmaceutical research. Alban. J. Pharma. Sci. 1(1), 9-15.
Selim, S., Al Jaouni, S., 2016. Anti-inflammatory, antioxidant and antiangiogenic activities of diosgenin isolated from traditional medicinal plant, Costus speciosus (Koen ex.Retz.) Sm. Nat. Prod. Res. 30(16), 1830-1833.
Suk, S., Kwon, G.T., Lee, E., Jang, W.J., Yang, H., Kim, J.H., Thimmegowda, N.R., Chung, M.Y., Kwon, J.Y., Yang, S., Kim, J.K., Park, J.H.Y., Lee, K.W., 2017. Gingerenone A, a polyphenol present in ginger, suppresses obesity and adipose tissue inflammation in high-fat diet-fed mice. Mol. Nutr. Food Res. doi: 10.1002/mnfr.201700139.
Suresh, P.K., Sucheta, S., Sudarshana, V.D., Selvamani, P., Latha, S., 2008. Antioxidant activity in some selected Indian medicinal plants. Afr. J. Biotechnol. 7, 1826-1828.
Syed, U., Ganapasam, S., 2017. Beneficial influence of ellagic acid on biochemical indexes associated with experimentally induced colon carcinogenesis. J. Cancer Res. Ther. 13(1), 62-68.
Xiong, Y.L., 2017. Inhibition of hazardous compound formation in muscle foods by antioxidative phytophenols. Ann. N. Y. Acad. Sci. doi: 10.1111/nyas.13368.
Yuan, Y., Pan, S., Yang, S.L., Liu, Y.L., Xu, Q.M., 2017. Antioxidant and cardioprotective effects of Ilex cornuta on myocardial ischemia injury. Chin. J. Nat. Med. 15(2), 94-104.
Zhu, J., Liang, Y., Yue, S., Fan, G., Zhang, H., Zhang, M., 2017. Combination of panaxadiol and panaxatriol type saponins and ophioponins from shenmai formula attenuates lipopolysaccharide-induced inflammatory injury in cardiac microvascular endothelial cells by blocking NF-kappa B pathway. J. Cardiovasc. Pharmacol. 69(3), 140-146.
