اثر هشت هفته تمرین هوازی و مصرف مکمل رزوراترول بر نشانگر اکسایشی (MDA) و آنتی اکسایشی(SOD) و (GPX) بافت کاردیومیوسیتی رت های نر دیابتی شده با استرپتوزوتوسین
محورهای موضوعی : مجله پلاسما و نشانگرهای زیستی
علی مهری
1
(سوپروایزر بالینی مجتمع اموزش درمانی بوعلی .
دانشگاه ازاد علوم پزشکی تهران)
کلید واژه: دیابت, MDA, تمرین هوازی, SOD, رزوراترولGPX,
چکیده مقاله :
چکیده زمینه و هدف: مطالعه حاضر اثر تمرین هوازی همراه مصرف مکمل رزوراترول بر فاکتورهای تنظیمی و اجرایی آپوپتوز کاردیومیوسیتی در رت های نر دیابتی شده با استرپتوزوسین است. روش کار: دریک کارآزمایی تجربی 42 موشصحرایی نر، تصادفی به 6 گروه7 تایی کنترل سالم، دیابتی ، دیابتی سالین ، مکمل رزوراترول ، تمرین هوازی و مکمل رزوراترول+تمرین هوازی تقسیم شدند. دیابت با تزریق تک دوزml/kg50 استرپتوزوتوسین القا شد. برنامه گروه های تمرین، تمرین و مکمل بر روی تریدمیل 5 روز در هفته که به مدت 8 هفته انجام شد پس از پایان دوره تمام حیوانات با شرایط کاملاً مشابه و به دنبال12-14 ساعت ناشتایی و 48 ساعت پس از آخرین جلسه تمرینی بیهوش و قربانی شدند. پس از خروج قلب و هموژنایز کردن آن غلظت (GPX،SOD،MDA) بصورت الایزا سنجش شد. یافته ها: پس از القای دیابت غلضت GPX و SODقلبی نسبت به کنترل سالم کاهش معنی دار یافت در حالیکه غلظت MDAدر این شرایط افزایش معنی دار داشت .اما تعامل تمرین و رزوراترول توانست اثر معنی داری بر غلظت SOD قلبی داشته باشد در حالی که تعامل این دو اثر معنی دار بر غلظت MDAوGPX قلبی نداشت . نتیجه گیری: تمرین هوازی و رزوراترول اثر سودمندی بر آپوپتوز قلبی در القای دیابت دارند اما تعامل این دو بر روند آپوپتوز قلبی معنادار نیست. البته از تمرین هوازی و رزوراترول میتوان بعنوان طب مکمل برای حفاظت میوکارد در برابر آپوپتوز نابجای ناشی از دیابت القا شده استفاده نمود.
Inroduction & Objective:The present study was conducted to investigate the effect of aerobic training with resveratrol supplementation on regulatory and executive factors of cardiomyocytes apoptosis in streptococci diabetic male rats Material and Method:In an experimental study, 50 male rats were randomly divided into 5 groups of 10 healthy controls, diabetics, resveratrol supplements, aerobic training and complementary resveratrol + aerobic training. Diabetes was induced by single injection of streptozotocin, ml / kg. The program of exercise, exercise and supplementation groups began for 8 weeks and 5 days a week on the training of Treadmill. All animals were completely and similarly treated, followed by 14-12hours of fasting and 48 hours after the last training session, anesthetized and sacrificed. After cardiac output and homogenization, the concentration (GPX, SOD, and MDA) was measured as ELISA. Results: After induction of diabetes, the concentration of GPX and SOD in the cardiac system was significantly lower than healthy controls, while MDA concentration increased significantly in these conditions. However, exercise interaction and resveratrol had a significant effect on the concentration of cardiac SOD, while the interaction of these two effects there was no concentration of MDA and GPX in the heart. Conclusion: Aerobic exercise and resveratrol have a beneficial effect on cardiac apoptosis in inducing diabetes, but their interaction is not significant in the process of cardiac apoptosis. Of course aerobic exercise and resveratrol can be used as complementary medicine for protecting myocardial cells against the invasive induced apoptosis induced by diabetes.
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