شناسائی و پراکنش عوامل بیماریزای ویروسی مولد موزائیک در مزارع لوبیاچیتی استان اصفهان
محورهای موضوعی : گیاه پزشکی
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کلید واژه: اصفهان, پراکندگی, شناسایی, virus, identification, Isfahan, لوبیا چیتی, ویروس, Frenchbean, Dispersion,
چکیده مقاله :
در یک بررسی سه ساله نمونههایی با نشانههای ابلقی، موزائیک، زردی و بدشکلی برگها از مزارع لوبیا چیتی در مناطق مختلف استان اصفهان جمع آوری گردید. نشانههای حاصل از مایه زنی شیره نمونهها روی گیاهان آزمون و نتایج بدست آمده از انجام آزمون سرولوژیک الیزا، نشانگر آلودگی نمونههای مذکور به ویروس موزائیک معمولی لوبیا (Bean Common mosaic virus, BCMV)، ویروس موزائیک خیار (Cucumber mosaic virus, CMV)، ویروس موزائیک یونجه (Alfalfa mosaic virus, AMV) و ویروس پیچیدگی برگ چغندر (Beet curly top virus, BCTV) بود. فراوانی ویروسهای مزبور با نمونه برداری از مرحله 4-2 برگی تا مرحله گلدهی تعیین گردید و مشاهده شد که ویروس موزائیک معمولی لوبیا در تمام مناطق لوبیا کاری استان از شایعترین عوامل ویروسی است. آلودگی اولیه بوتهها در مرحله 4-2 برگی به این ویروس بین 41/0 تا 31/2 درصد و در مرحله گلدهی بین 17/3 تا 16/23 درصد تعیین شد. فراوانی آلودگی مزارع لوبیا به ویروسهای موزائیک خیار و موزائیک یونجه در مرحله 4-2 برگی به ترتیب بین صفر و 17/0 درصد و در مرحله گلدهی به ترتیب بین 74/2 و 92/8 درصد بود. همچنین 34/1 درصد ازگیاهچه های پرورش یافته از بذرهای جمع آوری شده از مزارع مختلف لوبیا چیتی آلوده به ویروس موزائیک لوبیا و 02/0 درصد آلوده به ویروس موزائیک خیار بودند. آلودگی به ویروس موزائیک یونجه در این گیاهچه ها مشاهده نگردید.
In a 3-year investigation on french bean fields, samples with mosaic, mottling and yellowing symptoms were collected from French bean fields in various regions of Isfahan province .The symptoms resulted from inoculating samples to test plants and serological tests, indicated that the samples infected to Bean common mosaic virus (BCMV) , Cucumber mosaic virus (CMV), Alfalfa mosaic virus (AMV) and Beet curly top virus (BCTV). Frequency of the viruses was determined through sample collecting from 2 - 8 leaf till flowering stages it was indicated that 121BCMV was the prevalent virus in all French bean fields. Infection rate to BCMV in 2-4 leaf stage was 0.41-2.31% and in flowering stage was 3.7-23.16%.Infection of bean fields to CMV and AMV in 2-4 leaf stage were 0 and 0.17% and in flowering stage were 2.74 and 8.92%, respectively.In seed transmission test, 1.34% and 0.02% were infected to BCMV CMV respectively, and infection to AMV was not observed in the seedlings emerged from fied collected bean seeds.
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