بررسی ترکیبات فیتوشیمیایی و آنتی‌اکسیدانی اندام‌های مختلف گیاه دارویی Ferula assafoetida L. در دو رویشگاه طبیعی استان‌های سمنان و خراسان

محورهای موضوعی : بیوشیمی

مرتضی مهرپور ¹ (گروه کشاورزی، واحد دامغان، دانشگاه آزاداسلامی، دامغان، ایران)
بهاره کاشفی ² (گروه کشاورزی، واحد دامغان، دانشگاه آزاداسلامی، دامغان، ایران)
محمد مقدم ³ (استادیار، گروه علوم باغبانی، دانشگاه فردوسی مشهد، مشهد، ایران)

تاریخ دریافت : 1394/04/15 تاریخ پذیرش : 1394/06/05 تاریخ انتشار : 1395/03/01

کلید واژه: فنل, آنتی‌اکسیدان, فلاونوئید, تانن, آنغوزه, خراسان و سمنان,

چکیده مقاله :

این تحقیق با هدف بررسی فیتوشیمیایی و آنتی‌اکسیدانی اندام‌های مختلف گیاه آنغوزه (Ferula assafoetida L.) در دو استان‌ سمنان و خراسان رضوی در سال 1392 انجام گرفت. اندازه‌گیری میزان فنل‌‌کل با استفاده از روش فولین- سیوکالتیو، فلاونوئید کل با روش کلرید آلومینیوم‌، تانن (فولیندنیس) و ظرفیت آنتی‌اکسیدانی نمونه‌ها نیز با استفاده از روش‌های DPPH و FRAP اندازه‌گیری گردیدند. نتایج حاصل از تجزیه واریانس نشان داد که بیشترین میزان فنل‌ کل (014/1 و 8/2 میلی‌گرم گالیک اسید در یک گرم عصاره خشک) به‌ترتیب در عصاره برگ از استان خراسان و سمنان مشاهده گردید. حداکثر میزان تانن به‌ترتیب در عصاره‌های ساقه و برگ استان خراسان و سمنان مشاهده شد. بیشترین فعالیت آنتی‌اکسیدانی در روش DPPH (08/1111 و 61/731 میکروگرم بر میلی‌لیتر) به‌ترتیب در عصاره ریشه نمونه خراسان و عصاره ساقه سمنان مشاهده شد. به طورکلی، بالاترین میزان ترکیبات فنل و فلاونوئید کل به‌ترتیب در عصاره برگ و ریشه گیاه در استان سمنان بدست آمد. همچنین اندام ریشه در خراسان از بالاترین عملکرد آنتی‌اکسیدانی در مهار رادیکال‌های آزاد برخوردار بود. بررسی داده‌ها حاکی از آن است که کیفیت و کمیت ترکیبات شیمیایی و عملکرد آنتی‌اکسیدانی آنها بسته به نوع اندام، تنوع رویشگاهی و سپس روش‌های ارزیابی متفاوت است.

چکیده انگلیسی:

This study was evaluated in phytochemical and antioxidant activity of Ferula assafoetida L. in Semnan and Khorasan-Razavi provinces. The plant samples were collected in May 2013, the total phenolic  was determined by Folin–Ciocalteu, total flavonoid by Aluminium chloride , tannin contents  by Folin- Denis and the antioxidant capacity were measured utilizing DPPH and FRAP methods. Results of variance analysis were showed that the highest total phenols content (1.014 and 2.8 mgGAE/1 g) was observed in leave extract of Khorasan and Semnan provinces, respectively. The maximum tannin contents (0.00194 mg/ml) was observed in stem and leave extracts of Khorasan and Semnan provinces .Also the highest antioxidant activity by DPPH method (1111.08 and 731.61 µg/ml) was observed in root extract of Khorasan province samples. The maximum antioxidant activity content were obtained in leave extract of Semnan by FRAP method (2.85 mg/ml). In general the highest content of total phenol and flavonoid was obtained in leave and root extracts of plants in Semnan province. But the root extract of Khorasan had the maximum antioxidant capacity (1111.08 µg/ml).Therefore it may be stated that Mojen region in Semnan province had higher antioxidant activity than Khorasan region. So the quality of antioxidant compounds of Ferula assafoetida L. was depending on variation of environmental factors and plant parts.

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