ارزیابی میزان فنل و فلاونویید کل ,عملکرد آنتی اکسیدانی، اثرضد دردی و ضد التهابی عصاره های گیاه دارویی (basillicum var. purpurescens .Ocymum) بر موش های صحرایی نر در مقایسه با داروهای اپیوئیدی(مرفین)

محورهای موضوعی : گیاهان دارویی

سولماز فخاری ¹ (متخصص بیهوشی و فلوشیپ پالیاتیو، بیمارستان امام رضا، دانشگاه علوم پزشکی تبریز، تبریز، ایران)
عیسی بیله جانی ² (متخصص بیهوشی و فلوشیپ بیهوشی قلب، بیمارستان قلب شهید مدنی، دانشگاه علوم پزشکی تبریز، تبریز، ایران)
آرش خاکی ³ (گروه پاتولوژی، واحد تبریز،دانشگاه آزاد اسلامی، تبریز، ایران)
اصغر رجب زاده ⁴ (2کترای علوم تشریحی، گروه علوم تشریحی، دانشگاه علوم پزشکی لرستان، خرم آباد، ایران)
معصومه مازندرانی ⁵ (گروه زیست‌شناسی ،واحد گرگان، دانشگاه آزاد اسلامی، گرگان، ایران)

تاریخ دریافت : 1395/12/02 تاریخ پذیرش : 1396/02/10 تاریخ انتشار : 1396/03/01

کلید واژه: ریحان, آنتی‌اکسیدان‌, درد‌, داروهای اپیوئیدی (مرفین), فعالیت SOD‌, فنل و فلاونویید کل‌,

چکیده مقاله :

این تحقیق به منظور ارزیابی میزان فنل و فلاونویید کل، عملکرد آنتی‌اکسیدانی، اثر ضددردی و ضدالتهابی عصاره گیاه دارویی ریحان بنفش ( basillicum var. purpurescens.Ocymum) بر موش‌های صحرایی نر در مقایسه با داروهای اپیوئیدی (مرفین) انجام گرفت. در این مطالعه از موش‌های صحرایی نرنژاد ویستاردرمحدوده وزنی250±10 گرم استفاده گردید. به‌صورت تصادفی موش‌ها به پنچ گروه ده تایی تقسیم‌بندی شدند. گروه کنترل درمان شده با اسید استیک (1/0 میلی‌گرم بر کیلوگرم)، گروه‌های درمان شده با عصاره اتانولی ریحان با دوز‌های 7/0 و 9/0 و 1 میلی‌گرم/ کیلوگرم، مورفین (1/0 میلی‌گرم بر کیلوگرم) و تست ریتینگ استفاده شد. محتوای فنول و فلاونوئید کل در عصاره‌های آبی و اتانولی گیاه به‌ترتیب با روش های معرف فولین-سیوکالتیو و رنگ‌سنجی کلرید آلومینیوم، فعالیت آنتی اکسیدانی با استفاده از روشDPPH ارزیابی گردید. پروستاگلاندین PGE2 و سطح سرمی آنزیم SOD با کیت الایزا مورد بررسی قرار گرفتند. نتایج نشان داد عصاره اتانولی گیاه نسبت به عصاره آبی از بیشترین ترکیبات ثانویه فنلی (mg EGA/g 5/51±3/288) و فلاونوییدی (mg EQU/g 04/1±7/154) برخوردار بود و به واسطه کثرت سنتز متابولیت‌های ثانوی از بیشترین میزان مهار رادیکال‌های آزاد برخوردار بود (1/4±4/79). همبستگی مثبت معنی‌داری بین محتوای فنل و فلاونوئید کل گیاه با میزان ظرفیت آنتی اکسیدانی کل و درصد جاروب کنندگی رادیکال آزاد وجود داشت. (9/0r<، 05/0P<). با افزایش دوز تجویزی از عصاره ریحان، دردهای مزمن ایجاد شده کاهش چشمیگیری پیدا می‌کند (001/0.(P≤ همچنین سطح سرمی PGE2 در گروه‌های تیمار شده با عصاره ریحان و مرفین کاهش قابل توجهی را نسبت به گروه تیمار نشده نشان داد (036/0P=). سطح SOD نیز در گروه‌های درمان شده با عصاره نزدیک به گروه مورفین مشاهده شد (05/0P<). اثرات ضددردی و ضدالتهابی این عصاره از لحاظ آماری نزدیک به اثرات مرفین مشاهده شد. نتایج به‌دست آمده در تایید استفاده‌های سنتی گیاه به‌عنوان آنتی‌اکسیدان و ضدالتهاب قوی در درمان بیماری‌های التهابی و تسکین دردهای طولانی مدت مثل داروهای اپیوئیدی می‌باشد

چکیده انگلیسی:

This study was carried out to evaluate the total flavonoide, phenols, antioxidant, anti-pain and anti-inflammatory in different extracts of Ocimum ebasillicum var. purpurescens in comparison with opioid drug (morphine) in male rats. The male Wistar rats with 250±10 g weighing was used and randomly divided to five groups. Group I (treated by 0.1mg/kg acetic acid, II-IV (treated by 0.7, 0.9 and 1 mg/kgof plantextract) , V (treated by 0.1 mg/kg morphine) and evaluated by Writhing test. The total phenol and flavonoids content was evaluated by follin-ciocalteus reagent, Ammonium chloride, the antioxidant activity was measured by DPPH method  and  serum levels of PGE2 and SOD activity measured by ELISA Kits.  According to results, the ethanol extract of plant had more content of secondary metabolites with total phenol (288.3±51.5 mg EGA/g) content, flavonoide content (154.7±1.04mg EGU/g) and the highest ability of free radicals scavenging (79.4±4.1%). There was a significant correlation between phenol- flavonoid ingredients to antioxidant activity rate and free radicals scavenging of plant (P<0.05, r<0.9) extract. High dosages of plant extract could be decreased chronic pains, in addition, serum level of PGE2 has been showed a significantly decreasing in groups treated by Ocimum basilicum extract and morphine in compared to untreated group. The serum SOD of treated groups (plant extract) was observed near to morphine group (P<0.05). Anti-inflammatory and anti-pain effects of this plant statically were observed next to morphine. It is found that steadily consumption of herbal usage, as antioxidant and anti-inflammatory, could be medicated to inflammatory treatment and long-term pains instead of opioid drugs.

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