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  • مقایسه میزان فنل، فلانوئید کل و فعالیت آنتی‌اکسیدانی میوه چهار گیاه داروئی Momordica charantia L., Cucurbita pepo L. cultivar pumpkin, Ecballium elaterium (L.)A. Rich, Lagenaria siceraria L. cultivar Marankka

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کد مقاله : EJMP-1805-1355 (R6) بازدید : 266 صفحه: 26 - 39

20.1001.1.23223235.1397.6.4.3.5

نوع مقاله: پژوهشی

مقایسه میزان فنل، فلانوئید کل و فعالیت آنتی‌اکسیدانی میوه چهار گیاه داروئی Momordica charantia L., Cucurbita pepo L. cultivar pumpkin, Ecballium elaterium (L.)A. Rich, Lagenaria siceraria L. cultivar Marankka

محورهای موضوعی : فیتوشیمی

زینب محکمی 1 , عزیزاله خیری 2 , مهدی توکلی زاده اصفهانی 3 , محسن ثانی خانی 4 , عباس بهاری 5

1 - علوم باغبانی-دانشکده کشاورزی-دانشگاه زنجان-زنجان-ایران
2 - عضو هیئت علمی دانشگاه زنجان
3 - گروه فارماکگنوزی-دانشکده داروسازی-دانشگاه علوم پزشکی زنجان
4 - عضو هیئت علمی دانشگاه زنجان
5 - پژوهشکده زیست فناوری دانشگاه زنجان

تاریخ دریافت : 1397/02/23 تاریخ پذیرش : 1397/11/14 تاریخ انتشار : 1397/12/01

کلید واژه: آنتی‌اکسیدان, کارلا, خیار آب‌پران, کدوی هالووین, مارانکا,

چکیده مقاله :

این تحقیق با هدف ارزیابی محتوای فنل کل، فلاونوئید کل و فعالیت آنتی اکسیدانی میوه چهار گیاه دارویی کارلا ، خیار آب پران ، کدوی هالووین و کدوی مارانکا انجام گرفت. بدین منظور آزمایشی بصورت کاملاً تصادفی با سه تکرار اجرا گردید. بدین صورت که میوه های بالغ این گیاهان از مزرعه پژوهشی دانشگاه زنجان در بازه ی زمانی شهریور تا آذر 1396 برداشت گردید. شاخص های فیتوشیمیایی محتوای فنول کل(روش فولین سیکالتو)، فلاونوئید کل(روش آلومینیوم کلراید) و فعالیت آنتی اکسیدانی(روشDPPH) ارزیابی شدند. بالاترین محتوای فنلی و فلاونوئیدی از عصاره متانولی میوه ی خیار آب پران و کارلا به ترتیب با مقادیر 5/3 ±59/85 میلی گرم گالیک اسید بر وزن خشک و80/0±13/12میلی گرم کوئرستین بر وزن خشک حاصل شد. نتایج تست آنتی اکسیدانی نشان داد که عصاره متانولی کارلا به خوبی رادیکال های آزاد را مهار نمود (69/0± 09/97 درصد) و پس از آن خیار آب پران (1.5± 8/87 درصد) در مرتبه بعدی قرار داشت و کمترین فعالیت آنتی-اکسیدانی مربوط به عصاره متانولی میوه کدوی هالووین (7/2 ± 02/58 درصد) بود. افزون بر این همبستگی بسیار قوی بین محتوای فلاونوئیدی و فعالیت آنتی اکسیدانی (0.872= P) مشاهده گردید. همچنین همبستگی مثبتی بین محتوای فنلی و فعالیت آنتی اکسیدانی (0.545=P) وجود داشت. به طور کلی آنالیز نتایج این پژوهش نشان داد که عصاره کارلا و خیار آب پران غنی از ترکیبات فنلی و فلاونوئیدی بوده و بالاترین قدرت مهار رادیکال های آزاد را در مقایسه با سایر گونه ها داشته hند و می توان از آنها به عنوان غذا-دارو بهره برد.

چکیده انگلیسی:

ABSTRACT This study was designed to investigate the total phenolic content, total flavonoid content and antioxidant activity of fruits methanolic extracts from Momordica charantia L., Ecballium elaterium (L.) A.Rich, Cucurbita pepo L. cultivar pumpkin, Lagenaria siceraria L. cultivar Marankka belonging to Cucurbitaceae family. For this purpose, a factorial experiment was conducted in a completely randomized design with three replications. The matured fruits were harvested from the research farm in Zanjan university during the September to December 2017. Phytochemical characteristics of total phenol content (Folin–‌Ciocalteu method), total flavonoid (Aluminum Chloride method) and antioxidant activity (DPPH method) were evaluated. The highest level of phenolic and flavonoid contents was recorded for the fruit methanolic extracts from the Ecballium elaterium (L.) and Momordica charantia L. , with 85/5 ± 3/5 mg GAE/g and 12.13 ± 0.8, respectively. The results of DPPH test showed that the methanolic extract of Momordica charantia exhibited potent activity (97.09 % ± 0.96), followed by Ecballium elaterium (87.8% ± 1.5) and the lowest antioxidant activity related to methanolic extract of pumpkin fruit (58/02 % ± 2.7). Furthermore, there was a strong correlation between flavonoids content and antioxidant activity (P = 0.87) and also there was a positive correlation between phenolic content and antioxidant activity (P = 0.54). Generally, the phytochemical analysis showed that, Ecballium and Karella extracts are rich in polyphenol compounds against other sspecies and can be used as food.drug-

منابع و مأخذ:


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