بررسی تاثیر کودهای اسید فولویک و اسید آمینه بر ترکیبات فنلی ، فلاونوئیدی، فعالیت آنتی اکسیدانی و رنگیزه های فتوسنتزی گیاه دارویی (.Coriandrum sativum L)
محورهای موضوعی : زراعت و اصلاح نباتاتمحمد حسین امینی فرد 1 , مژگان غلامی 2 , حسن بیات 3 , فرید مرادی نژاد 4
1 - استادیار گروه علوم باغبانی و مرکز پژوهشی گیاهان ویژه منطقه دانشکده کشاورزی، دانشگاه بیرجند
2 - دانشجوی کارشناسی ارشد، رشته علوم باغبانی، گرایش فیزیولوژی گیاهان دارویی، ادویه‏ای و عطری. دانشکده کشاورزی دانشگاه بیرجند
3 - گروه علوم باغبانی، دانشکده کشاورزی دانشگاه بیرجند، بیرجند ایران
4 - دانشیار گروه علوم باغبانی و مرکز پژوهشی گیاهان ویژه منطقه دانشکده کشاورزی، دانشگاه بیرجند
کلید واژه: کود آلی, آنتوسیانین, متابولیتهای ثانویه, مواد هیومیکی,
چکیده مقاله :
این تحقیق به منظور بررسی تأثیر کودهای اسیدفولویک و اسیدآمینه بر فعالیت آنتیاکسیدانی و صفات بیوشیمیایی گیاه دارویی گشنیز، بهصورت فاکتوریل در قالب طرح بلوکهای کامل تصادفی در مزرعه دانشکده کشاورزی دانشگاه بیرجند اجرا شد. فاکتورها شامل اسید فولویک بصورت مصرف خاکی در سه سطح (صفر، 5 و 10 کیلوگرم در هکتار) و اسید آمینه بصورت محلولپاشی در دو سطح (0 و 3 در هزار) با سه تکرار بودند. نتایج نشان داد که اسید فولویک تاثیر معنی داری بر فعالیت آنتیاکسیدانی، میزان فنل، فلاونوئید، آنتوسیانین، کربوهیدرات ورنگیزههای فتوسنتزی(کلروفیل a، b ، کلروفیل کل و کارتنوئید) گیاه داشت، بهطوریکه بیشترین میزان فعالیت آنتی اکسیدانی گیاه (9/77 درصد) با کاربرد 10 کیلوگرم در هکتار اسید فولویک وکمترین میزان آن (1/65 درصد) از تیمار شاهد بدست آمد. اسید آمینه نیز بر میزان آنتوسیانین، فلاونوئید، فعالیت آنتیاکسیدانی و کلروفیل a و کلروفیل کل گیاه تاثیرگذار بود و ملاحظه شد که بیشترین میزان فلاونوئید از تیمار سه درهزار اسیدآمینه (13/8 میلیگرم برگرم) بدست آمد. اثرات متقابل اسید فولویک و اسید آمینه نیز بر فعالیت آنتیاکسیدانی، میزان آنتوسیانین، فلاونوئید، کلروفیل a و کلروفیل کل گیاه تاثیر معنی داری داشت، بهطوریکه بیشترین میزان آنتوسیانین(84/1میلیگرم برگرم) از تیمار 5 کیلوگرم اسید فولویک و اسید آمینه 3 در هزار و کمترین میزان آنتوسیانین(87/0 میلیگرم برگرم) از تیمار شاهد بدست آمد. به طور کلی، نتایج بیانگر تاثیر مثبت اسید فولویک و اسید آمینه بر صفات بیوشیمیایی و فعالیت آنتی اکسیدانی گیاه دارویی گشنیز بود.
The effects of applications of fulvic acid and amino acid on biochemical characteristics of coriander were evaluated under field conditions. Treatments were tow levels of amino acid (0 and 3 per thousand) and three levels of fulvic acid (0, 5 and 10 kg ha-1). The experiment was designed as factorial arrangement based on randomized complete block design with three replications at the Research station of Faculty of Agriculture University of Birjand. The results showed that fulvic acid had significant effect on biochemical characteristics (antioxidant activity, flavonoids phenol, anthocyanin, carbohydrate, chlorophyll a, b, total chlorophyll and carotenoid), so that the highest of antioxidant activity (77.9 %) was obtained from with application of 10 kg/ ha fulvic acid and the lowest antioxidant activity (65.1 %) was observed from control treatment. Amino acid also affected the antioxidant activity, flavonoids, chlorophyll a and total chlorophyll content. The highest of flavonoids (8.13 mg/g) was obtained with application of 3 per thousand of amino acid. The results of interactions showed that fulvic acid and amino acid had significant effect on antioxidant activity, flavonoids, anthocyanin, chlorophyll a and total chlorophyll, so that, the highest of anthocyanin (1.84 mg/g) were obtained by with application of 3 per thousand of amino acid and 5kg ha-1 fulvic acid and the lowest of anthocyanin (0.87 mg/g) was observed from control treatment. In general, the results indicated a positive effect of fulvic acid and amino acid on the biochemical traits of coriander.
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