بررسی و مقایسه ترکیبات فنلی و فعالیت آنتی‌اکسیدانی اندام های مختلف گیاه دارویی (Calotropis procera Aiton)در دو منطقه بم و جیرفت

محورهای موضوعی : گیاهان دارویی

سمیه غلامشاهی ¹ (کارشناس‌ارشد، گروه علوم باغبانی، دانشگاه آزاد اسلامی واحد جیرفت، جیرفت، ایران.)
علی صالحی ساردویی ² (گروه علوم باغبانی، دانشگاه کشاورزی و منابع طبیعی گرگان)

تاریخ دریافت : 1397/05/23 تاریخ پذیرش : 1398/03/25 تاریخ انتشار : 1398/03/01

کلید واژه: DPPH, ترکیبات فنلی, فعالیت آنتی‌اکسیدانی, IC50,

چکیده مقاله :

گیاهان منبع غنی از ترکیبات فنلی (فلاونوئیدها، تانن و آنتوسیانین) هستند که مهم‌ترین آنتی‌اکسیدان‌های طبیعی به شمار می‌آیند. آنتی‌اکسیدان‌های موجود در رژیم غذایی به لحاظ محافظت بدن در مقابل استرس اکسیداتیو و حفظ سلامت حائز اهمیت هستند. این تحقیق به منظور بررسی کمی و کیفی ترکیبات فنلی و آنتی‌اکسیدانی گیاه استبرق (Calotropis procera Aiton) به عنوان جایگزین آنتی‌اکسیدان‌های سنتزی انجام شده است. اندام‌های مختلف گیاه از جمله برگ، گل، میوه و شیره گیاه در مرحله بلوغ کامل در دو منطقه جرفت و بم مورد ارزیابی قرار گرفت. فعالیت آنتی‌اکسیدانی عصاره گیاه با استفاده از روش مهار رادیکال آزاد 2 و 2- دی فنیل 1- پیکریل هیدرازیل (DPPH) اندازه‌گیری شد. پس از تهیه عصاره متانولی سنجش میزان فنلی به روش اسپکتروفتومتری صورت گرفت و در نهایت تجزیه واریانس داده‌ها با استفاده از نرم افزار SAS انجام شد و میانگین‌ها از طریق آزمون دانکن در سطح 05/0P≤ مقایسه شد .نتایج بدست آمده نشان دادند ترکیبات فنلی و آنتی‌اکسیدانی در اندام برگ بیشتر از سایر اندام‌ها بود و در منطقه بم نسبت به جیرفت بیشتر بود. که این نتایج در میوه هم به همین صورت بود. اما در گل و شیره (لاتکس) استبرق بومی جیرفت ترکیبات فنلی بیشتری را نشان داد. ترکیبات فنلی و آنتی‌اکسیدانی برگ در مقایسه با شیره 5/2 برابر بود. لاتکس (شیره) استبرق در دو منطقه بم و جیرفت نسبت به سایر اندام‌ها (برگ، گل، میوه) کمترین میزان ترکیبات فنلی را داشتند. فعالیت آنتی‌اکسیدانی با میزان ترکیبات فنلی رابطه مستقیم دارد.

چکیده انگلیسی:

Plants are rich sources of phenolic compounds (flavonoids, tannins and anthocyanins) in the most important natural antioxidants. Dietary antioxidants to protect the body against stress and health are important oxiddative. This study was designed to investigate the quality and quantity of phenolic compounds and antioxidant milkweed plants as an alternative to synthetic antioxidants has been done. After preparing the methanolic extract, the phenol concentration was measured by spectrophotometry and finally, the antioxidant activity of the extract was measured using radical 2 and 2-diphenyl-1-picaril hydrazil (DPPH) method. Analysis of variance was performed using SAS software and the means by Duncan's test at P> 0/05 were compared. The results obtained showed that the phenolic The results showed that, the phenolic and antioxidant compounds in leaf were more than other organs and so higher the Bam region than Jiroft region. The results were the same applies to fruit. But the flowers and sap (latex) more phenolic compounds found in Jiroft native milkweed. Phenolic compounds and antioxidant activity of leaf sap compared with 2/5 times. Latex (sap) Milkweed in Bam and Jiroft region Compared with organs (leaves, flowers, fruits) had the lowest total phenolic compounds. The results of this study medicinal plant milkweed and antioxidant phenolic compounds as natural antioxidants and can be used in pharmaceutical and food industry.

منابع و مأخذ:

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