Antioxidant Effects of Thyme (Thymus vulgaris) Extract on Ram Sperm Quality during Cryopreservation
الموضوعات :
و. واحدی
1
(Department of Animal Science, Moghan College of Agriculture and Natural Resources, University of Mohaghegh Ardabili, Ardabil, Iran)
ن. هدایت ایوریق
2
(Department of Animal Science, Faculty of Agricultural Science, University of Mohaghegh Ardabili, Ardabil, Iran)
م. بهروزلک
3
(Department of Animal Science, Faculty of Agriculture, Payame Noor University, Tehran, Iran)
ع. دیرنده
4
(Department of Animal Science, Faculty of Agriculture, Sari Agricultural Sciences and Natural Resources University, Sari, Iran)
الکلمات المفتاحية: Antioxidant, freeze-thawing, ram semen, <, i>, Thymus vulgaris<, /i>, extract,
ملخص المقالة :
Oxidative stress during freezing-thawing reduces sperm motility, viability, membrane functions and antioxidant capacity. Thymus vulgaris has antioxidant properties due to phenolic compounds. The purpose of current study was to evaluate the effect of Thymus vulgaris extract as a natural antioxidant on post-thawed Moghani ram sperm quality. Semen samples were collected twice a week from four rams. Samples were then pooled and diluted with Tris based extender supplemented with different concentrations of Thymus vulgaris (0, 2, 4, 8, 12 and 16 mL per dL diluent solutions). Following the cooling and equilibration stages for semen samples, the samples were frozen and stored in liquid nitrogen until evaluation. Following this freezing procedure, samples were thawed, and the sperm motility and velocity parameters, viability and plasma membrane integrity were evaluated. Our results showed that the addition of 2 and 4 mL/dL extracts at the extender stage increased the total motility percentages (P<0.05). Addition of 4 mL/dL extract improved progressive motility and percentages of viability compared to the control group and high levels of extract groups (P<0.05). Plasma membrane integrity of sperm in 4 and 8 mL/dL extract groups were greater (P<0.05) than the control and 12 and 16 mL/dL extract groups. Also, addition, 16 mL/dL extract had a significantly negative effect on all evaluated traits (P<0.05). In conclusion, supplementation of extender with intermediate amounts of Thymus vulgaris extract improves the quality of ram sperm after freezing and thawing in a dose-dependent manner.
Abd El Kader M.A. and Mohamed N.Z. (2012). Evaluation of protective and antioxidant activity of thyme (Thymus vulgaris) extract on paracetamol-induced toxicity in rats. Australian J. Basic Appl. Sci. 6, 467-474.
Ahmed N.A.H., Nahed M. and El-Aal R.A. (2015). Assessing the effect of thyme and rosemary as antiaflatoxicosis on fertility in male rats. J. Am. Sci. 11(12), 294-302.
Alasadiy Y.D.K. (2014). Study of the biological effect of Thymus vulgaris extracts on spermatogenesis in experimentally infected white mice Balb/c by toxoplasma gondii. Int. J. Sci. Engin. Res. 5, 547-553.
Ashrafi I., Kohram H. and Ardabili F.F. (2013). Anti-oxidative effects of melatonin on kinetics, microscopic and oxidative parameters of cryopreserved bull spermatozoa. Anim. Reprod. Sci. 139, 25-30.
Baghshahi H., Riasi A., Mahdavi A.H. and Shirazi A. (2014). Antioxidant effects of clove bud (Syzygium aromaticum) extract used with different extenders on ram spermatozoa during cryopreservation. Cryobiology. 69, 482-487.
Baranauskiene R., Venskutonis P.R., Viskelis P. and Dambrauskiene E. (2003). Influence of nitrogen fertilizers on the yield and composition of thyme (Thymus vulgaris). J. Agric. Food Chem. 51, 7751-7758.
Bucak M.N., Ateşşahin A., Varışlı Ö., Yüce A., Tekin N. and Akçay A. (2007). The influence of trehalose, taurine, cysteamine and hyaluronan on ram semen: microscopic and oxidative stress parameters after freeze–thawing process. Theriogenology. 67, 1060-1067.
Bucak M.N., Tuncer P.B., Sarıözkan S., Başpınar N., Taşpınar M., Çoyan K., Bilgili A., Akalın P.P., Büyükleblebici S., Aydos S. and Ilgaz S. (2010). Effects of antioxidants on post-thawed bovine sperm and oxidative stress parameters: antioxidants protect DNA integrity against cryodamage. Cryobiology. 61, 248-253.
Bunker V. (1992). Free radicals, antioxidants and ageing. J. Lab. Sci. 49, 299-312.
Cao G., Giovanoni M. and Prior R.L. (1996). Antioxidant capacity in different tissues of young and old rats. Physiol. Soc. Exp. Biol. Med. 211, 359-365.
Curry M.R., Millar J.D. and Watson P.F. (1994). Calculated optimal cooling rates for ram and human sperm cryopreservation fail to confirm with empirical observations. Biol. Reprod. 51, 1014-1021.
Daghigh Kia H., Farhadi R., Ashrafi I. and Mehdipour M. (2016). Anti-oxidative effects of ethanol extract of Origanum vulgare on kinetics, microscopic and oxidative parameters of cryopreserved Holstein bull spermatozoa. Iranian J. Appl. Anim. Sci. 6, 789-783.
Duh P.D. and Yen G.C. (1997). Antioxidant efficacy of methanolic extracts of peanut hulls in soybean and peanut oils. J. Am. Oil Chem. Soc. 74(6), 745-748.
El-Nekeety A.A., Mohamed S.R., Hathout A.S., Hassan N.S., Aly S.E. and Abdel-Wahhab M.A. (2011). Antioxidant properties of Thymus vulgaris oil against aflatoxin-induce oxidative stress in male rats. Toxicon. 57, 984-991.
Evans G. and Maxwell W.M.S. (1990). Salamon's Artificial inSemination of Sheep and Goats. University Press, Sydney, Australia.
Hamzawy M.A., EL-Denshary E.S.M., Hassan N.S., Manaa F. and Abdel Wahhab M.A. (2012). Antioxidant and renoprotective effects of Thyme Vulgaris extracts in rats during aflatoxicosis. Global J. Pharm. 6, 106-117.
Haselmeyer A., Zentek J. and Chizzola R. (2015). Effects of thyme as a feed additive in broiler chickens on thymol in gut contents, blood plasma, liver and muscle. J. Sci. Food Agric. 95(3), 504-508.
Hudaib M., Speroni E., Di Pietra A.M. and Cavrini V. (2002). GC/MS evaluation of thyme (Thymus vulgaris) oil composition and variations during the vegetative cycle. J. Pharm. Biomed. Anal. 29, 691-700.
Jackson S.A.L. and Hay R.K.M. (1994). Characteristics of varieties of thyme (Thymus vulgaris) for use in the UK: Oil content, composition and related characters. Ann. Hort. Sci. 69, 275-281.
Jeong S.Y., Kim D.R., Kim K.C., Nam D.U. and Ahn Lee S.C. (2004). Effect of seed roasting conditions on the antioxidant activity of defatted sesame meal extracts. Food Chem. Toxicol. 69, 377-381.
Kheradmand Garmsir A., Zarehshahneh A., Jalali S.M.A., Nouri H. and Afshar A. (2014). Effects of dietary thyme (Thymus vulgaris) and fish oil on semen quality of miniature Caspian horse. J. Equine Vet. Sci. 34, 1069-1075.
Khodaei Motlagh M., Sharafi M., Zhandi M., Mohammadi-Sangcheshmeh A., Shakeri M., Soleimani M. and Zeinoaldini S. (2014). Antioxidant effect of rosemary (Rosmarinus officinalis) extract in soybean lecithin-based semen extender following freeze–thawing process of ram sperm. Cryobiology. 69, 217-222.
Kulisic T., Radonic A. and Milos M. (2005). Antioxidant properties of thyme (Thymus vulgaris L.) and wild thyme (Thymus serpyllum) essential oils. Italian J. Food Sci. 17(3), 315-321.
Lamirande D.E., Jiang H., Zini A., Kodama H. and Gagnon C. (1997). Reactive oxygen species and sperm physiology. Rev. Reprod. 2(1), 48-54.
Letchamo W., Xu H.L. and Gosselin A. (1995). Variations in photosynthesis and essential oil in thyme. J. Plant Physiol. 147, 29-37.
Leung A. and Foster S. (1996). Encyclopedia of Common Natural Products used in Food, Drugs and Cosmetics. John Wiley and Sons, Inc., New York.
Loziene K., Venskutonis P.R., Sipailien A. and Labokas J. (2007). Radical scavenging and antibacterial properties of the extracts from different Thymus pulegioides chemotypes. Food Chem. 103, 546-559.
Naijian H.R., Kohram H., Shahneh A.Z., Sharafi M. and Bucak M.N. (2013). Effects of different concentrations of BHT on microscopic and oxidative parameters of Mahabadi goat semen following the freeze–thaw process. Cryobiology. 66(2), 151-155.
Pournazari M., Qotbi A.A., Seidavi A. and Corazzin M. (2017). Prebiotics, probiotics and thyme (Thymus vulgaris) for broilers: Performance, carcass traits and blood variables. Rev. Colomb. Cienc. Pecu. 30(1), 3-10.
Purdy P.H. (2006). A review on goat sperm cryopreservation. Small Rumin. Res. 63, 215-225.
Radwan Nadiia L., Hassan R.A., Qote E.M. and Fayek H.M. (2008). Effect of ntural antioxidant on oxidative stability of eggs and productive and Reproductive performance of laying hens. Int. J. Poult. Sci. 7, 134-150.
Revell S. and Mrode R. (1994). An osmotic resistance test for bovine semen. Anim. Reprod. Sci. 36, 77-86.
Roby M.H.H., Sarhan M.A., Selim K.A.H. and Khalel K.I. (2013). Evaluation of antioxidant activity, total phenols and phenolic compounds in thyme (Thymus vulgaris), sage (Salvia officinalis), and marjoram (Origanum majorana) extracts. Ind. Crop. Prod. 43, 827-831.
Sariozkan S., Bucak M.N., Tuncer P.B., Buyukleblebici S., Eken A. and Akay C. (2015). Influence of fetuin and hyaluronan on the post-thaw quality and fertilizing ability of Holstein bull semen. Cryobiology. 71, 119-124.
SAS Institute. (2004). SAS®/STAT Software, Release 9.1. SAS Institute, Inc., Cary, NC. USA.
Schafer S. and Holzmann A. (2000). The use of transmigration and spermac stain to evaluate epididymal cat spermatozoa. Anim. Reprod. Sci. 59, 201-211.
Schwarz K., Ernst H. and Ternes W. (1996). Evaluation of antioxidative constituents from thyme. J. Sci. Food Agric. 70(2), 217-223.
Shahidi F. (1997). Natural antioxidants: An overview. Pp. 1-10 in Natural Antioxidants, Chemistry, Health Effects and Applications. F. Shahidi, Ed. AOCS Press, Champaign, Illinois, USA.
Shanoon A.K. and Mahdi S.J. (2012). Effects of Thymus vulgaris and Zingiber officinale aqueous on semen parameters, testes weight and histology measurements of broiler breeder male. Int. Poult. Sci. 11(9), 594-598.
Sinha M.P., Sinha A.K., Singh B.K. and Prasad P.L. (1996). The effect of glutathione on the motility, enzyme leakage and fertility of frozen goat semen. Theriogenology. 41, 237-243.
Stahl-Biskup E. and Saez F. (2004). Thyme. Handbook of Herbs and Spices. CRC Press, Wood head Publishing Limited, Cambridge, United Kingdom.
Stradaioli G., Noro T., Sylla L. and Monaci M. (2007). Decrease in glutathione (GSH) content in bovine sperm after cryopreservation: comparison between two extenders. Anim. Reprod. Sci. 67, 1249-2155.
Uysal O. and Bucak M.N. (2007). Effects of oxidized glutathione, bovine serum albumin, cysteine and lycopene on the quality of frozen-thawed ram semen. Acta Vet. Brno. 76(3), 383-390.
Wang Y., Sharma R. and Agarwal A. (1997). Effect of cryopreservation and sperm concentration on lipid peroxidation in human semen. Urology. 50, 409-413.
Watson P.F. (2000). The cause of reduced fertility with cryopreserved semen. Anim. Reprod. Sci. 61, 481-492.
Youdim K.A. and Deans S.G. (1999). Dietary supplementation of thyme (Thymus vulgaris) essential oil during the lifetime of the rat: its effects on the antioxidant status in liver, kidney and heart tissues. Mech. Age .dev. 109(3), 163-175.
Zanganeh Z., Zhandi M., Zare-Shahneh A., Najafi A., Nabi M.M. and Mohammadi-Sangcheshmeh A. (2013). Does rosemary aqueous extract improve buck semen cryopreservation? Small Rumin. Res. 114(1), 120-125.
