بررسی اثرات آنتی اکسیدانی عصاره آویشن بر فراسنجه‌های کیفی اسپرم منجمد-یخ‌گشایی‌شده قوچ مغانی

Subject Areas : Camel

و. واحدی ¹ (Department of Animal Science, Moghan College of Agriculture and Natural Resources, University of Mohaghegh Ardabili, Ardabil, Iran)
ن. هدایت ایوریق ² (Department of Animal Science, Faculty of Agricultural Science, University of Mohaghegh Ardabili, Ardabil, Iran)
م. بهروزلک ³ (Department of Animal Science, Faculty of Agriculture, Payame Noor University, Tehran, Iran)
ع. دیرنده ⁴ (Department of Animal Science, Faculty of Agriculture, Sari Agricultural Sciences and Natural Resources University, Sari, Iran)

Received: 2017-07-12 Accepted : 2017-09-30 Published : 2018-06-01

Keywords: آنتی‌اکسیدان, عصاره آویشن, منجمد-یخ‌گشایی, اسپرم قوچ,

Abstract :

استرس اکسیداتیو طی فرآیند انجماد-یخ‌گشایی اسپرم به وجود آمده و باعث کاهش تحرک، زنده مانی، عملکردهای غشایی، ظرفیت آنتی اکسیدانی و در نهایت باروری سلول­های اسپرم می­شود. گیاه آویشن به دلیل داشتن ترکیبات فنولی دارای خاصیت آنتی‌اکسیدانی است. هدف این پژوهش بررسی تأثیر عصاره گیاه آویشن به عنوان یک آنتی‌اکسیدان طبیعی بر کیفیت اسپرم منجمد-یخ‌گشایی شده قوچ مغانی بود. در این مطالعه از 4 رأس قوچ مغانی هفته‌ای دو بار اسپرم‌گیری شد. سپس نمونه‌های منی به طور یکسان با هم مخلوط شدند و با رقیق‌کننده بر پایه تریس که حاوی سطوح مختلف عصاره آویشن (0، 2، 4، 8، 12 و 16 میلی‌لیتر در دسی‌لیتر محلول رقیق‌کننده) بود، رقیق شدند. نمونه‌ها پس از طی مراحل سردسازی و انجماد تا زمان ارزیابی در داخل ازت مایع نگهداری شدند. بعد از یخ‌گشایی، پارامترهای حرکتی اسپرم، زنده‌مانی و تست یکپارچگی غشاء مورد ارزیابی قرار گرفت. نتایج نشان داد که افزودن سطوح 2 و 4 دسی‌لیتر/میلی‌لیتر عصاره درصد تحرک کلی اسپرم­ها را افزایش داد (05/0P<). همچنین غلظت 4 دسی‌لیتر/میلی‌لیتر عصاره به طور معنی‌داری تحرک پیش‌رونده و درصد زنده‌مانی اسپرم­ها را نسبت به گروه شاهد و گروه­های تیماری حاوی سطوح بالاتر بهبود داد (05/0P<). درصد یکپارچگی غشای پلاسمایی اسپرم‌ها در گروه­های 4 و 8 دسی‌لیتر/میلی‌لیتر در مقایسه با گروه شاهد و گروه­های 12 و 16 به طور معنی‌داری بالاتر بود (05/0P<). همچنین افزودن 16 دسی‌لیتر/میلی‌لیتر به طور معنی‌داری اثر منفی بر تمامی صفات ارزیابی شده داشت (05/0P<). در نتیجه استفاده از عصاره آویشن در رقیق‌کننده منی منجر به بهبود کیفیت اسپرم منجمد-یخ‌گشایی‌شده قوچ شد که این اثر وابسته به دوز می‌باشد.

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