Study of DGAT1 Gene Polymorphisms with Carcass Traits in Iranian Zel and Lori-Bakhtiari Sheep Breeds
الموضوعات :
م. صادقی
1
(Department of Animal Science, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran)
م. مخبر
2
(Department of Animal Science, Faculty of Agriculture, Urmia University, Urmia, Iran)
م. مرادی-شهر بابک
3
(Department of Animal Science, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran)
و. سلطانی
4
(Department of Animal Science, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran)
م. بهروزلک
5
(Department of Animal Science, Faculty of Agriculture, Payame Noor University, Tehran, Iran)
الکلمات المفتاحية: Iran, Sheep, PCR-SSCP, Zel, <i>DGAT1</i> gene, Lori-Bakhtiari,
ملخص المقالة :
Diacylglycerol acyltransferase1 (DGAT1) plays an important role in the metabolism of triglycerides which catalyze the final step of triglyceride synthesis in animals. The objective of this study was to investigate the single-nucleotide polymorphisms (SNPs) in 5'UTR, exon-1, and exon-2 of DGAT1 in two Iranian indigenous sheep breeds. A total of 309 animals including fat-tailed Lori-Bakhtiari (n=152) and thin-tailed Zel (n=157) were used in this study. The genotypic patterns were detected by polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP). Five SSCP patterns were detected for 5'UTR and exon-1 fragment by PCR-SSCP and subsequently confirmed by sequencing PCR products. The sequencing results revealed that there are three novel polymorphisms in 5'UTR and exon-1fragment of DGAT1 at the studied breeds. Out of the detected polymorphisms only A277G substitution in exon-1 of DGAT1 leads to the changes in amino acids (p.Arg26Gly). There was significant correlation (p < 0.05) between fat-tail weight (FTW) and back-fat thickness (BFT) and the observed genotypes in Lori-Bakhtiari breed; therefore, animals with G5 pattern had higher FTW and BFT compared to G1 pattern. The G1 and G5 genotypic patterns or haplotypes were different at their position 101 of 5'UTR region. No significant relationship (p < 0.05) was found between the detected genotypes of 5'UTR and exon-1 fragment of DGAT1 in Zel breed and carcass traits. These results revealed that detected DGAT1 novel SNPs had significant effects on carcass traits and they can be used as a marker for these traits.
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