مطالعه چندشکلی ژن DGAT1 و ارتباط آن با صفات لاشه در گوسفندان نژادهای ایرانی لری-بختیاری و زل

Subject Areas : Camel

م. صادقی ¹ (Department of Animal Science, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran)
م. مخبر ² (Department of Animal Science, Faculty of Agriculture, Urmia University, Urmia, Iran)
م. مرادی-شهر بابک ³ (Department of Animal Science, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran)
و. سلطانی ⁴ (Department of Animal Science, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran)
م. بهروزلک ⁵ (Department of Animal Science, Faculty of Agriculture, Payame Noor University, Tehran, Iran)

Received: 2019-01-01 Accepted : 2019-03-16 Published : 2020-09-01

Keywords: ایران, PCR-SSCP, گوسفند, زل, ژن <i>DGAT1</i>, لری-بختیاری,

Abstract :

دی آسیل گلیسرول آسیل ترانسفراز1 (DGAT1) نقش مهمی در متابولیسم تری­گلیسریدها دارد و مرحله­ پایانی مسیر سنتز تری­گلیسرید در حیوانات را کاتالیز می­کند. هدف مطالعه­ حاضر بررسی چندشکلی­های تک نوکلئوتیدی (SNPs) در نواحی 5'UTR، اگزون 1 و اگزون 2 در دو نژاد گوسفند بومی ایران بود. به این منظور، از تعداد 309 حیوان شامل گوسفند دنبه دار لری-بختیاری (n=152) و گوسفند بدون دنبه زل (n=157) استفاده شد. الگوهای ژنوتیپی با استفاده از تکنیک PCR-SSCP شناسایی شد. پنج الگوی ژنوتیپی در نواحی 5'UTR و اگزون 1 شناسایی شد. وجود تفاوت در الگوهای مختلف با استفاده از تکنیک توالی­یابی تأیید گردید. نتایج توالی­یابی حاکی از وجود سه پلی­مورفیسم در نواحی 5'UTR و اگزون 1 نژادهای مورد بررسی بود. از میان نواحی دارای چندشکلی، تنها جایگزینی مربوط به موقعیت 277 در اگزون 1 منجر به تغییر اسید آمینه­ آرژنین به گلایسین شد (p.Arg26Gly). ارتباط معنی­داری (P<0.05) بین ژنوتیپ‌های شناسایی شده در نژاد لری-بختیاری و وزن دنبه (FTW) و ضخامت چربی پشت (BFT) وجود داشت و حیوانات با الگوی ژنوتیپی G5 عملکرد بالاتری نسبت به الگوی ژنوتیپیG1 داشتند. این دو الگوی ژنوتیپی یا هاپلوتیپ تنها در موقعیت 101 ناحیه­ 5'UTR تفاوت دارند. هیچ ارتباط معنی­داری (05/0P<) بین الگوهای ژنوتیپی مشاهده شده (قطعه 5'UTR و اگزون 1) و صفات لاشه نژاد زل مشاهده نشد. این نتایج نشان داد که SNPهای جدید شناسایی شده­ DGAT1 اثر معنی­داری بر روی صفات لاشه دارند و می­توانند به ­عنوان نشانگر برای این صفات استفاده شوند.

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