Effect of Different Concentration and Application Methods of Polyamines (Putrescine, Spermine, Spermidine) on Some Morphological, Physiological, and Enzymatic Characteristics and Vase Life of Rosa hybrida cv. ‘Dolce Vita’ Cut Flower
الموضوعات : مجله گیاهان زینتی
Elham Danaee
1
(Department of Horticulture, Collage of Agriculture, Garmsar Branch, Islamic Azad University, Garmsar, Iran.)
Vahid Abdossi
2
(Department of Horticulture, Collage of Agricultural Sciences and Food Technology, Science and Research Branch, Islamic Azad University, Tehran, Iran.)
الکلمات المفتاحية: Rose, superoxide dismutase, Polyamines, Phenylalanine ammonia- lyase,
ملخص المقالة :
In order to study on different methods of application of polyamines (putrescine, spermine, spermidine) on some morphological, physiological, enzymatic and vase life of Rosa hybrida cv. ‘Dolce Vita’, experiment was conducted as a factorial experiment in a completely randomized design with 32 treatments, 3 replicates and each replication containing 5 vases, a total of 480 vases were executed. First factor was, putrescine, spermine, spermidine (50, 100 mg/l) and second factor was 4 methods of uses (pre-harvest spray, postharvest spray, 24 hrs pulse and continuous). We are 2 blanks in this experiment: distilled water and 3 % sucrose + 3 mg l-1 nanosilver. Morphological, physiological and enzymatic traits such as relative fresh weight, cell membrane stability index, anthocyanin of petals, total chlorophyll of leaves, protein, phenylalanine ammonia- lyase and superoxide dismutase enzymes activity and vase life were measured. The results showed that Spm100 mg/l in pre-harvest spray method had the highest rates in protein, activity of phenylalanine ammonia- lyase and superoxide dismutase enzymes. Therefore, according to the results of this study, the method of pre-harvest spraying of polyamines can be recommended as the best way to improve morphological, physiological, enzymatic and vase life of Rosa hybrida cv. ‘Dolce Vita’.
Ananieva, E.A., Christov, K.N. and Popova, L.P. 2004. Exogenous treatment with salicylic acid leads to increased antioxidant capacity in leaves of barley plants exposed to paraquat. Plant Physiology, 161: 319-328.
Arnon, D. I. 1949. Copper enzymes in isolated chloroplasts. Polyphenoloxidase in Beta vulgaris. Plant Physiology, 24: 1-15.
Basiri, Y., Zaheri, H. and Mashayekhi, K. 2010. Effect of nano-silver treatments on vase life of cut flower of carnation. Journal of Advanced Laboratory Research in Biology. 2 (2): 40-44.
Bosma, T. and Dole, J.M. 2002. Post- harvest handling of cut Campanula medium flowers. Horticultural Science, 37: 954-958.
Bhattacharjee, S.K. and De, L.C. 2005. Postharvest technology of flowers and ornamental plants. Aavishkar Publishers. P: 8-306.
Bradford, M.M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 72: 248-254.
Celickel, F.G. and Ried, M.S. 2002. Postharvest handling of stock (Matthiola incana). Hort Science, 37: 144-147.
Chance, B. and Maehly, A.C. 1955. Assay of catalase and peroxidase. Methods in Enzymology. SP Colowick, NO Kaplan, Eds. 2: 764-775.
Esna Ashari, M. and Zokaee Khosroshahi, M. R. 2008. Polyamines and horticulture science. Bu-Ali Sina University Press, Hamadan.
Ezhilmathi, K., Singh, V., Arora, P. and Sairam, R.K. 2007. Effect of 5-sulfosalicylic acid on antioxidant in relation to vase life of gladiolus cut flower. Plant Growth Regulation, 51: 99-108.
Farjadi Shakib, M., Naderi, R.A. and Mashhadi Akbar Bojar, M. 2013. Effect of spraying spermidine on morphological, physiological and biochemical traits of Syclamen persicum. Plant Ecophysiology (Arsanjan Branch), 5 (13): 96-113.
Figueroa, I., Colinas, M.T., Mejia, J. and Remirez Cien, F. 2005. Post-harvest physiological changes in roses of different vase life. Cienciae Investigación Agraria, 32: 167-176.
Hatfield, R. and Vermerris, W. 2001. Lignin formation in plants. The dilemma of linkage specificity. Plant Physiology. 126: 1351-1357.
Hoseini Farahi, M., Eshghi, S., Kavousi, B., Amiri Fahliani, R. and Dastyaran, M. 2013. Effect of spermidine and calsium sulphate on qualitative, quantitative and vase life of Rosa in hydroponic system. Journal of Science and Technology of Greenhouse Crops, 14: 15-25.
Ismaeilkhan Zandi, M. R. and Danaee, E. 2016. The effect of polyamines spraying on some qualitative, quantitative and enzymic traits on rose flower (Rosa hybrida) cv. Black Baccara on the plant. Cellular and Molecular Plant Biology Journal, 10 (1&2): 25-33.
Kafi, M. and Ghasemi Ghahsareh, M. 2007. Scientific and practical floriculture, Ayeezh Press.
Meng, X. 2004. Relation of flower development and anthocyanin accumulation in Gerbera hybrida. Journal of Horticultural Science and Biotechnology, 79 (1): 131-137.
Mittler, R. 2002. Oxidative stress, antioxidants and stress tolerance. Trends in Plant Science. 7: 405-410.
Nemeth, M., Janda, T., Horvath, E., Paldi, E. and Szalai, G. 2002. Exogenous salicylic acid increases polyamine content but may decrease drought tolerance in maize. Plant Science, 162: 569-574.
Rashidi, A. 2008. Complete guide to rose planting and maintenance. Amazon Publishers. P: 48.
Redman, R.S., Freeman, S., Elifton, D.R., Morre, D.J., Brown, G. and Rodringuez, R.J. 1999. Biochemical analysis of plant protection afforded by a non pathogenic endophytic mutant of Colletotrichum magna. Plant Physiology, 119: 795-804.
Senaranta, T., Touchell, D., Bunn, E. and Dixon, K. 2000. Acetyl salicylic acid and salicylic acid induce multiple stress tolerance in bean and tomato plants. Plant Growth Regulation, 30: 157-161.
Singh, A., Kumar, J. and Kumar, P. 2008. Effect of plant growth regulators and sucrose on postharvest physiology, membrane stability and vase life of cut spikes of Gladiolus. Journal of Plant Growth Regulation, 55: 221-229.
Zobideh, A., Habibi, F. and Mortazavi, S.N.A. 2014. Effect of spraying putrescine and spermine on enhancing vase life of Alstroemeria cv. Sukari. Journal of Breeding Plant, 17 (1): 241-255.
