The Effect of Interval and Continued Trainings with Crocin on Apoptotic Markers in the Heart Tissue of High-Fat Diet and Streptozotocin Induced Type 2 Diabetic Rats
الموضوعات : Report of Health CareGhobad Hassanpour 1 , Hojaat Allah Nik bakht 2 , Mohammad Ali Azarbayjani 3 , Nader Shakeri 4 , Hossein Abednazari 5
1 - Department of Exercise Physiology, Science and Research Branch, Islamic Azad University, Tehran, Iran
2 - Department of Exercise Physiology, Science and Research Branch, Islamic Azad University, Tehran, Iran
3 - Department of Exercise Physiology, Central Tehran Branch, Islamic Azad University, Tehran, Iran
4 - Department of Exercise Physiology, Science and Research Branch, Islamic Azad University, Tehran, Iran
5 - Department of Exercise Physiology, Science and Research Branch, Islamic Azad University, Tehran, Iran
الکلمات المفتاحية: Training, Diabetes, Apoptosis, Crocin,
ملخص المقالة :
Introduction: Diabetes is a metabolic disease that has a close relationship with increased physical disabilities and muscle tissue damage. The aim of this study was to investigate the effect of interval and continued trainings with crocin consumption on Bcl-2, Bax and P53 gene expression of heart tissue in high-fat diet- and streptozotocin induced type 2 diabetic rats. Methods: In this experimental study, 49 adult high-fat diet- and streptozotocin induced diabetic rats were selected and randomly assigned to 7 groups (1) high intensity interval training (HIIT) (2) low intensity continued training (LICT) (3) HIIT with crocin consumption, (4) LICT with crocin consumption, (5) crocin consumption, (6) sham and (7) control. HIIT and LICT groups ran the treadmill for eight weeks, three sessions per week with intensity 80- 85 and 50- 55 percent of maximum running speed, respectively, and crocin consumption groups received crocin 25 mg/kg per week for eight weeks. To analyze the research findings, paired sample t-test, two- way ANOVA with Bonferroni post hoc test were used (p≤0.05). Results: Eight weeks of HIIT and LICT had significant effect on reduction of Bax gene expression in heart tissue of diabetic rats (p≤0.05), and HIIT had significant effect on reduction of P53 gene expression in heart tissue of diabetic rats (p≤0.05), crocin consumption had significant effect on reduction of P53 gene expression in heart tissue of diabetic rats (p≤0.05), endurance training with crocin consumption had interaction effect on increase of Bcl-2 gene expression and reduction of P53 gene expression in heart tissue of diabetic rats (p≤0.05). Conclusion: It seems that interval and continued with crocin consumption in high- fat diet- and streptozotocin induced diabetic rats have interaction anti-apoptotic effects.
1. Lohrasebi M, Edalatmanesh MA, Hosseini SA, Farkhaie F, Salehi M, Kazemi N, et al. Lipid lowering effects of coriandrum sativum extract and endurance training in streptozotocin induced diabetic rats. Rep Health Care. 2016; 2 (2): 31- 40.
2. Eckel RH, Kahn SE, Ferrannini E, Goldfine AB, Nathan DM, Schwartz MW, et al. Obesity and type 2 diabetes: what can be unified and what needs to be individualized?. JCEM. 2011; 96 (6): 1654- 1663.
3. Ndumele CE, Cobb L, Lazo M, Bello NA, Shah A, Nambi V, et al. Weight history and subclinical myocardial damage. Clin Chem. 2018; 64 (1): 201- 209.
4. Fernández-Armenteros JM, Gómez-Arbonés X, Buti-Soler M, Betriu-Bars A, Sanmartin-Novell V, Ortega-Bravo M, et al. Psoriasis, metabolic syndrome and cardiovascular risk factors. a population-based study. J Eur Acad Dermatol Venereol. 2018; 28: 1- 8.
5. Kanter M, Aksu F, Takir M, Kostek O, Kanter B, Oymagil A. Effects of low intensity exercise against apoptosis and oxidative stress in streptozotocin-induced diabetic rat heart. Exp Clin Endocrinol Diabetes. 2017; 125 (9): 583- 591.
6. Al-Rasheed NM, Al-Rasheed NM, Bassiouni YA, Hasan IH, Al-Amin MA, Al-Ajmi HN, et al. Simvastatin ameliorates diabetic nephropathy by attenuating oxidative stress and apoptosis in a rat model of streptozotocin-induced type 1 diabetes. Biomed Pharmacother. 2018; 31 (105): 290- 298.
7. Kim DY, Jung SY, Kim CJ, Sung YH, Kim JD. Treadmill exercise ameliorates apoptotic cell death in the retinas of diabetic rats. Mol Med Rep. 2013; 7 (6): 1745- 1750.
8. Seyedgomi F, Bashiri J, Gholami F. Effect of high intensity endurance training on p53 and cytochrome-c gene expression in
male rat soleus muscle. Armaghane Danesh J. 2017; 22 (5): 608- 622.
9. Zhao G, Zhu Y, Eno C O, Liu Y, DeLeeuw L, Burlison J A, Li C. Activation of the proapoptotic Bcl-2 protein Bax by a small molecule induces tumor cell apoptosis. Mol Cell Biol. 2014; 34 (7): 1198- 1207.
10. Karimi MN, Abbasalipourkabir R, Arab Sadeghabadi Z, Ziamajidi N. The level of gene expression of Bax and Bcl-2 and the activity of caspase 3 in the liver tissues of normal, type 1 and type 2 diabetic rats before and after treatment with aqueous extract of garlic. JSSU. 2017; 25 (7): 547- 555.
11. Hoseini SA, Zar A, Kheirdeh M, Arayesh A. Effect of endurance training on vaspine and glycemic indexes in diabetic rats. Qom Univ Med Sci J. 2017; 10 (11): 17- 24.
12. Hoseini A, Zar A, Mansouri A. Effect of aloevera with swimming training on the alanine aminotransferase and aspartate aminotransferase levels of diabetic rats. IJNSFT. 2016; 11 (4): 29- 38.
13. Tanoorsaz S, Behpour N, Tadibi V. Investigating the effect of mid-term of aerobic exercise on apoptosis biomarkers in the cardiomyocytes of streptozotocin-induced diabetic rats. J Fasa Univ Med Sci. 2018; 7 (4): 488- 497.
14. Hosseini SA, Nikbakht H, Azarbayjani MA. The effect of aqua extract of saffron with resistance training on glycemic indexes of streptozotocin induced diabetic rats. Armaghane Danesh J. 2013; 18 (4): 284- 294.
15. Adibfar E, Hoseini S, Salehi O, Farkhaie F, Shahhosseini P. Lipid lowering effects of saffron aqua extract in diabetic rats. Rep Health Care. 2016; 2 (3): 9- 18.
16. Fallah Huseini H, Zaree A, Heshmat R, Bagher ALM, Fakhrzadeh H, et al., The effect of citrullus colocynthis (L.) schrad. fruit on oxidative stress parameters in type II diabetic patients. J Med Plants. 2006; 5: 55- 60.
17. Khosravi A, Mirzai B, Mehrabani J, Rasoulian B. The interaction effects of aerobic training and saffron extracts consumption on anti-oxidant defense system of heart and brain premotor cortex of young male rats following an acute bout of exhaustive endurance exercise. Sports Physiol. 2015; 7 (25): 109-130.
18. Yaribeygi H, Mohammadi M. Protective effect of crocin on kidney performance in chronic uncontrolled hyperglycemia-induced nephropathy in rat. J Zanjan Univ Med Sci Health Serv. 2017; 25 (109): 36- 49.
19. Mohammadi E, Mehri S, Badie Bostan H, Hosseinzadeh H. Protective effect of crocin against d-galactose-induced aging in mice. Avicenna J Phytomed. 2018; 8 (1): 14- 23.
20. Fernandes T, Magalhaes FC, Everton CC, et al. Aerobic exercise training inhibits skeletal muscular apoptotic signaling mediated by vegf-vegr2 in spontaneously hypertensive rats. Revista Brasileira de Medicina do Esporte. 2012; 18 (6): 412- 418.
21. Parco MS, Bryner RW, Martyn JK, Alway SE. Apoptotic adaptations from exercise training in skeletal and cardiac muscles. FASEB J. 2004; 1- 25.
22. Krijnen PAJ, Simsek S, Niessen HWM. Apoptosis in diabetes. Apoptosis. 2009; 14 (12): 1387– 1388.
23. Lee SC, Pervaiz S. Apoptosis in the pathophysiology of diabetes mellitus. Int J Biochem Cell Biol. 2007; 39 (3): 497- 504.
24. Anuradha R, Saraswati M, Kumar KG, Rani SH. Apoptosis of beta cells in diabetes mellitus. DNA Cell Biol. 2014; 33 (11): 743- 748.
25. Tomita T. Apoptosis in pancreatic β-islet cells in type 2 diabetes. BJBMS. 2016; 16 (3): 162- 179.
26. Kwak HB. Effects of aging and exercise training on apoptosis in the heart. J Exerc Rehabil. 2013; 9 (2): 212- 219.
27. Rahimi M, Shekarforoush S, Asgari AR, Khoshbaten A, Rajabi H, Bazgir B, et al. The effect of high intensity interval training on cardioprotection against ischemia-reperfusion injury in wistar rats. EXCLI J. 2015; 14: 237- 246.
28. Farkhondeh T, Samarghandian S, Shaterzadeh Yazdi H, Samin F. The protective effects of crocin in the management of neurodegenerative diseases: a review. Am J Neurodegener Dis. 2018; 7 (1): 1- 10.
29. Cheng SM, Ho TJ, Yang AL, Chen IJ, Kao CL, Wu FN, et al. Exercise training enhances cardiac IGFI-R/PI3K/Akt and Bcl-2 family associated pro-survival pathways in streptozotocin-induced diabetic rats. Int J Cardiol. 2013; 167 (2): 478- 485.
30. Lee YI, Choo JY, Kim MH, Kim KB, Lee DJ Lee KS. Effects of exeruse Trainingon pathological camdiac hypertrophy ye lated gene expression an apoptosis. Eurd App physiol. 2006; 97 (2): 216- 224.
31. Lee SD, Shyu WC, Cheng IS, Kuo CH, Chan YS, Lin YM, et al. Effects of exercise training on cardiac apoptosis in obese rats. Res Support. 2012; 23 (6): 566- 573.
32. Ziaaldini MM, Koltai E, Csende Z, Goto S, Boldogh SW, Taylor A, et al. Exercise training increases anabolic and attenuates catabolic and apoptotic processes in aged skeletal muscle of male rats. Ex Gerontology J. 2015; 67: 9- 14.
33. Phaneuf S, Leeuwenburgh C. Apoptosis and exercise. Med Sci Sports Exerc. 2001; 33: 393- 396.
34. Siagian M, Lousiana M, Santoso DIS, Endardjo S. Effects of anaerobic exercise and detraining on the caspase-3 expression of rat ventricular cardiomyocyte. Med J Indones. 2015; 24 (2): 84- 90.
35. Seo H, Chun- Hyung P, Seokrip Ch, Woocheol K, Byung- Duk J, Seungpil R. Effects of voluntary exercise on apoptosis and cortisol after chronic restraint stress in mice. J Exerc Nutrition Biochem. 2016; 20 (3): 16- 23.
36. Ghorbanzadeh V, Mohammadi M, Mohaddes G, Dariushnejad H, Chodari L. Effect of crocin and voluntary exercise on P53 protein in pancreas of type2 diabetic rats. Pharm Sci. 2017; 23: 182- 188.
37. Xiang M, Yang M, Zhou C, Liu J, Li W, Qian Z. Crocetin prevents AGEs-induced vascular endothelial cell apoptosis. Pharm Res. 2006; 54: 268- 274.
38. Sadoughi SD. Effect of crocin on Bax/Bcl-2 ratio, lipid peroxidation and antioxidant enzymes activity in liver tissue of chick embryo treated with silver nanoparticles. Horizon Med Sci J. 2017; 23 (4): 293- 299.
39. Salahshoor MR, Khashiadeh M, Roshankhah S, Kakabaraei S, Jalili C. Protective effect of crocin on liver toxicity induced by morphine. Res Pharm Sci.
2016; 11 (2): 120- 129.
40. Khosravi A, Omidali OA, Rasoulian B, Choobineh S. The effects of short- term aqueous saffron extracts consumption on malondialdehyde and anti-oxidant system content of liver of young male rats following an acute bout of exhaustive exercise. Yafte J. 2017; 19 (1): 20- 30.
41. Vakili A, Eianali MR, Bandegi AR. The protective effects of saffron against the oxidative damage in a transient model of focal cerebral ischemia in rats. Tehran Univ Med Sci J. 2011; 69 (7): 405- 412.
42. Rahbani Nobar M, Mohajeri D, Rezaie A, Rezaei Moghadam A. Effect of ethanolic extract of crocus sativus L. (saffron) stigma on serum levels of functional liver markers and hepatic tissue injury in streptozotocin-induced diabetic rats. Veter Clin Path. 2011; 5, 3(19): 1305-1313.
43. Varmazyar M, Azarbayjani M. The Effect of saffron supplementation of antioxidant enzymes activities during a session eccentric exercise in active males. JMP. 2014; 2 (50): 54- 63.
