پتانسیل ضد میکروبی و آنتی اکسیدانی کشت کالوس Convolvulus microphyllus Sieb

Subject Areas : Journal of Medicinal Herbs, "J. Med Herb" (Formerly known as Journal of Herbal Drugs or J. Herb Drug)

بوسکی پانچولی ¹ (گروه بیوتکنولوژی، دانشگاه کوتا، راجستان، هند.)
ام گوتام ² (گروه بیوتکنولوژی، دانشگاه کوتا، راجستان، هند.)

Received: 2022-02-19 Accepted : 2022-05-14 Published : 2022-04-21

Keywords: Antifungal, DPPH, FRAP, Antibacteria, antioxidant potentials,

Abstract :

Background & Aim: Convolvulus microphyllus Sieb. ex Spreng. (Family: Convolvulaceae) known as “Shankhpushpi” is reported to be brain tonic and useful in CNS disorder, hypertension, thyrotoxicosis and ulcer. Tissue culture technique has been optimized for the large scale production of medicinally important plant independent from climatic and geographic conditions. Antioxidant and antimicrobial activities were performed and compared with in vivo. Active compounds with biological activities were isolated, identified from the callus extracts. These biological constituents can be propagated in large amount with low cost use tissue culture techniques. Experimental: Cell cultures of C. microphyllus have been established using Murashige and Skoog’s (MS) medium supplemented with different concentrations of 2,4-dichlorophenoxyacetic acid (2, 4-D). Callus was harvested at different time intervals of 2, 4, 6 and 8 weeks and their antimicrobial and antioxidant potentials along with the isolation of active compounds isolation were carried out using established protocols.Results: Ethanolic extracts of 2 weeks-old callus demonstrated appreciable antifungal activity against Penicillium chrysogenum and Tricophyton rubrum (inhibition zone of 14.66 ± 0.66 and 14.00 ± 0.57 mm respectively) while maximum antibacterial activity was recorded in 6 and 4 weeks-old callus against Klebsiella pneumoniae (Inhibition zone of 14.66 ± 0.61 mm and 14.33 ± 0.59 mm respectively). Antioxidant potentials were more in plant extract (IC50 0.055 mg/ml and 510 ± 20.02 ascorbic acid equivalents) as compared to callus. Phenolic acids viz., caffeic-, p- coumaric-, ferulic-, gallic-, vanillic- and syringic acids were isolated and screened for antimicrobial efficacy.Recommended applications/industries: The callus extract shows similar results as that of In vivo plant. Two week old callus exhibit most profound antifungal and 4 to 6 week for antimicrobial activities. Callus extract shows similar bio-potentials and secondary metabolites level, so it can be used for large scale production of biologically active phytochemicals with antimicrobial properties.

References:

Adamczak, A., Ozarowski, M., and Karpinski, T. M. 2020. Antibacterial activity of some flavonoids and organic acidswidely distributed in plants. Journal of Clinical Medicine, 9(1): 109-126.

Ahmad, S., Zafar, R. and Shahid. M. 2007. Anticonvulsant potential of callus of Convolvulus microphyllus Sieb. Oriental Pharmacy and Experimental Medicine, 7(1): 46-50.

Albuquerque, B. R., Heleno, S. A., Oliveira, M. B. P. P., Barros, L., and Ferreira, I. C. F. R. 2021. Phenolic compounds: current industrial applications, limitations and future challenges. Food and Function, 12: 14–29.

Alvarado-Martinez, Z., Bravo, P., Kennedy, N. F., Krishna, M., Hussain, S. and Young, A. C. 2020. Antimicrobial and antivirulence impacts of phenolics on Salmonella enterica serovar Typhimurium. Antibiotics, 9:668-684.

Andrade, M., Benfeito, S., Soares, P., Magalhães e Silva, D., Loureiro, J. and Borges, A. 2015. Fine-tuning of the hydrophobicity of caffeic acid: studies on the antimicrobial activity against Staphylococcus aureus and Escherichia coli. RSC Advances, 5:53915.

Barar, F.S.K. and Sharma, V.N. 1965. Preliminary pharmacological studies on Convolvulus microphyllus Chois.-An Indian indigenous herb. Part I. Indian Journal of Physiology and Pharmacology, 9: 99-102.

Benzie, I.F.F. and Strain, J.J. 1999. Ferric reducing/antioxidant power assay: direct measure of total antioxidant activity of biological fluids and modified version for simultaneous measurement of total antioxidant power and ascorbic acid concentration. In Methods in Enzymolology; Packer, L., Ed.; Academic Press: Orlando, USA, pp. 15-27.

Bhattacharya, D., Ghosh, D., Bhattacharya, S., Sarkar, S., Karmakar, P., Koley, H. and Gachhui, R. 2018. Antibacterial activity of polyphenolic fraction of Kombucha against Vibrio cholerae: targeting cell membrane. Letters in Applied Microbiology, 66: 145–152. 

Bisht, N.P.S., Singh, R., Bansal, R.K. and Singh, P. 1978. Isolation of n-triacontane, aliphatic alcohols and stosterols from Convolvulus microphyllus Sieb. Zeitschrift für Naturforschung, 33B: 249.

Bouarab-Chibane, L., Degraeve, P., Ferhout, H., Bouajila, J., and Oulahal, N. 2018. Plant antimicrobial polyphenols as potential natural food preservatives. Journal of the Science and Food Agriculture. 99: 1457–1474.

Boyanava, L., Gergova, G., Nikolov, R., Derejian, S., Lazarova, E., Katsarov, N., Mitov, I. and Krastev, Z. 2005. Activity of Bulgarian propalis against 94 Helicobacter pylori strains in vitro by agar well diffusion, agar dilution and disc diffusion methods. Journal of MedicalMicrobiology, 54(5): 481-483.

Bray, H.G. and Thorpe, W.V. 1954. IN Glick, D. (ed.): Methods of Biochemical Analysis 1, John Wiley & Sons, New York, USA, pp. 27-57.

Chaturvedi, M., Mali, P.C. and Dixit, V.P. 1995. Hypolipidaemic effect of Convolvulus microphyllus in cholesterol fed gerbils (Meriones hurrinae Jerdon). Journal of Phytological Research, 8: 153-155.

Eibl, R., Meier, P., Stutz, I., Schildberger, D., Huehn, T. and Eibl, D. 2018. Plant cell culture technology in the cosmetics and food industries: Current state and future trends. Applied Microbiology and Biotechnology, 102(20): 8661-8675.

Fogliano, V., Verde, V., Randazzo, G. and Ritieni, A. 1999. A method for measuring antioxidant activity and its application to monitoring the antioxidant capacity of wines. Journal of Agriculture and Food Chemistry, 47: 1035-1040.

Mora-Pale, M., Bhan, N., Masuko, S., James, P., Wood, J. and McCallum, S., 2015. Antimicrobial mechanism of resveratrol-trans dihydrodimer produced from peroxidase-catalyzed oxidation of resveratrol. Biotechnology and Bioengineering, 112: 2417–2428.

Murashige, T. and Skoog, F. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum, 15: 473-497.

Oulahal N. and Degraeve P. 2022. Phenolic-Rich Plant Extracts With Antimicrobial Activity: An Alternative to Food Preservatives and Biocides? Frontiers in Microbiology. https://doi.org/10.3389/fmicb.2021.753518

Parihar, M.S. and Hemnani, T. 2003. Phenolic and antioxidant attenuate hippocample neuronal cell damage against kainic acid induced excitotoxicity. Journal of Biosciences, 28:121-128.

Pereira, J.A., Pereira, A.P.G., Ferreira, I.G.F.R., Valentap, P., Andrade, P.B., Seabra, R., Estevinho, L. and Bento, A. 2006. Table olives from Portugal; phenolic compounds, antioxidant potential and antimicrobial activity. Journal of Agriculture and Food Chemistry, 54:8425–8431.

Pernin, A., Guillier, L., and Dubois-Brissonnet, F. 2019. Inhibitory activity of phenolic acids against Listeria monocytogenes: deciphering the mechanisms of action using three different models. Food Microbiology. 80: 18–24.

Phin, C.K., Rossall, S. and Atong, M. 2009. In vitro synergy effect of syringic acid, caffeic acid and 4-hydroxybenzoic acid against Ganoderma boninense. International Journal of Engineering and Technology, 1(4): 282-284.

Rice-Evans, C.A., Miller, N.J., Bolwell, G.P., Bramley, P.M. and Pridham, J.B. 1995. The relative antioxidant activities of plant-derived polyphenolic favonoids. Free Radical Research, 22:375-383.

Rischer, H., Nohynek, L., Puupponen, R., Joselin Aguiar, P., Rocchetti, G., José, L.L., Câmara, S.,  Cruz, T.M.,  Marques, M.B. and Granato, D. 2022. Plant cell cultures of Nordic berry. species: Phenolic and carotenoid profiling and biological assessments. Food Chemistry, 366(1): 130571-130582.

Sairam, K., Rao, Ch. V. and Goel, R.K. 2001. Effect of Convolvulus pluricaulis Chois. on gastric ulceration, and secretion in rats. Indian Journal of Experimental Biology, 39: 350-354.

Shah, V.C. and Bole, P.V. 1960. Further pharmacognostic studies on the drug “Shankhpushpi”. Journal of the University of Bombay, 29: 154-165.

Singh, R.H., Sinha, B.N., Sarkar, F.H. and Udupa, K.N. 1979. Comparative biochemical studies on the effect of four Medhya Rasayana drugs described by Caraka on some central neurotransmitters in normal and stressed rates. Indian Journal of Research in Homeopathy, 14(3): 7-14. 

Srivastava, D.N. and Despande, S.M. 1975. Gas chromatographic identification on fatty acids, fatty alcohols and hydrocarbons of Convolvulus microphyllus (Chois.). Journal of American Oil Chemists’s Society, 52:318-319.

Margraf, T., Karnopp, A.R., Rosso, D. and Granato, S. 2015. Comparison between Folin-Ciocalteu and Prussian Blue assays to estimate the total phenolic content of juices and teas using 96-well microplates Journal of Food Science, 80(11): C2397-C2403.

Wen, A., Delaquis, P., Stanich, K., and Toivonen, P. 2003. Antilisterial activity of selected phenolic acids. Food Microbiology 20: 305–311.

Wu, X., Beecher, G.R., Holden, J.M., Haytowitz, D.B., Gebhardt, S.E. and Prior, R.L. 2004. Lipophilic and hydrophilic antioxidant capacities of common foods in the United States. Journal of Agricultural and Food Chemistry, 52: 4026-4037.

Yen, G.C. and Chen, H.Y. 1995. Antioxidant activity of various tea extracts in relation to their antimutagenicity. Journal of Agricultural and Food Chemistry, 43: 27-32.

Zafar, R., Ahamad, S. and Mujeeb, M. 2005. Estimation of Scopoletin in Leaf and Leaf Callus of Convolvulus Microphyllus Sieb. Indian Journal of Pharmaceutical Sciences, 67(5): 562-564.

Zhishen, J., Mengcheng, T. and Jianming, W. 1999. The determination of flavonoid content in mulberry and their scavenging effect on superoxide radicals. Food Chemistry, 64(4): 555-559.