آنالیز هورمون رشد در بزهای نژاد آلپاین و سانن با استفاده از روش PCR-SSCP

Subject Areas : Camel

ر. خاتمی نژاد ¹ (Department of Animal Science, Faculty of Agriculture, GonbadUniversity, Gonbad, Iran)
س. یوسفی ² (Department of Animal Science, Gorgan University of Agricultural Science and Natural Resources, Gorgan, Iran)
م. آهنی آذری ³ (Department of Animal Science, Gorgan University of Agricultural Science and Natural Resources, Gorgan, Iran)

Received: 2012-09-24 Accepted : 2012-12-31 Published : 2014-03-01

Keywords: بز, چند شکلی ژنتیکی, هورمون رشد,

Abstract :

در این مطالعه به منظور بررسی چند ‌شکلی ژن هورمون رشد به ‌عنوان یک ژن کاندیدا، نمونه‌های خون به‌ طور تصادفی از 34 بز نژاد آلپاین و 42 بز نژاد سانن به‌ دست آمد. DNA از نمونه‌های گرفته شده استخراج و ناحیه 365 جفت بازی از اگزون 5 ژن هورمون رشد توسط واکنش زنجیره‌ای پلی‌مراز تکثیر شد. محصولات PCR توسط روش SSCP بر روی ژل پلی اکریل آمید مورد آنالیز قرار گرفتند. نتایج آنالیز نشان داد که برای ژن مورد مطالعه چهار الگوی باندی متفاوت G1، G2،G3  و G4 به‌ ترتیب با فراوانی‌های 38/0 ،21/0 ،23/0 و 18/0 در بز آلپاین و 48/0، 21/0 ، 17/0 و 14/0 در بز نژاد سانن مشاهده شدند.

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